Cyclovirus ZM32

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Human associated cyclovirus 8; Cyclovirus VN

Average proteome isoelectric point is 8.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097ZPG6|A0A097ZPG6_9CIRC ATP-dependent helicase Rep OS=Cyclovirus ZM32 OX=1506566 GN=Rep PE=3 SV=1
MM1 pKa = 7.72ANNSTVRR8 pKa = 11.84RR9 pKa = 11.84FCWTLNNYY17 pKa = 8.05TDD19 pKa = 3.69EE20 pKa = 5.01DD21 pKa = 4.2VDD23 pKa = 4.45VLQKK27 pKa = 11.03DD28 pKa = 3.5LAEE31 pKa = 4.3LCKK34 pKa = 10.04FAIFGRR40 pKa = 11.84EE41 pKa = 3.98TCPSTGTKK49 pKa = 9.84HH50 pKa = 6.5LQGFCNLQRR59 pKa = 11.84PKK61 pKa = 10.35RR62 pKa = 11.84FNSIRR67 pKa = 11.84QIFGGRR73 pKa = 11.84AHH75 pKa = 7.27IEE77 pKa = 3.81RR78 pKa = 11.84AKK80 pKa = 11.13GSDD83 pKa = 3.64LEE85 pKa = 4.17NQEE88 pKa = 4.26YY89 pKa = 10.11CSKK92 pKa = 11.24GGDD95 pKa = 3.09VWQCGSPSTQGTRR108 pKa = 11.84TDD110 pKa = 3.47LQKK113 pKa = 10.72VVSVIEE119 pKa = 4.21GGEE122 pKa = 3.88RR123 pKa = 11.84NIKK126 pKa = 10.07NLALQFPTAYY136 pKa = 9.14IKK138 pKa = 10.39YY139 pKa = 9.66FKK141 pKa = 10.74GIEE144 pKa = 3.78QYY146 pKa = 10.83IRR148 pKa = 11.84IAHH151 pKa = 6.42GNPEE155 pKa = 4.25RR156 pKa = 11.84DD157 pKa = 3.42FKK159 pKa = 11.37TEE161 pKa = 3.7VFYY164 pKa = 10.55FWGATGTGKK173 pKa = 9.99SRR175 pKa = 11.84KK176 pKa = 9.34ARR178 pKa = 11.84EE179 pKa = 3.79EE180 pKa = 3.77SRR182 pKa = 11.84ALGDD186 pKa = 3.09TYY188 pKa = 11.22YY189 pKa = 10.96KK190 pKa = 10.5PRR192 pKa = 11.84GEE194 pKa = 3.75WWDD197 pKa = 3.93GYY199 pKa = 9.14TGQPCVVIDD208 pKa = 5.82DD209 pKa = 4.49FYY211 pKa = 11.88GWIKK215 pKa = 10.75YY216 pKa = 10.28DD217 pKa = 4.1EE218 pKa = 4.44LLKK221 pKa = 10.71ICDD224 pKa = 3.7RR225 pKa = 11.84YY226 pKa = 10.06PYY228 pKa = 9.86RR229 pKa = 11.84VPVKK233 pKa = 10.11GGYY236 pKa = 9.35EE237 pKa = 4.03NFVSKK242 pKa = 9.88YY243 pKa = 8.77IWITSEE249 pKa = 3.94RR250 pKa = 11.84PLEE253 pKa = 3.78EE254 pKa = 3.95VYY256 pKa = 10.77RR257 pKa = 11.84FLAYY261 pKa = 10.18DD262 pKa = 3.7CSSLRR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84LNKK272 pKa = 9.94EE273 pKa = 3.29IHH275 pKa = 6.23FEE277 pKa = 3.93

Molecular weight: 32.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097ZPG6|A0A097ZPG6_9CIRC ATP-dependent helicase Rep OS=Cyclovirus ZM32 OX=1506566 GN=Rep PE=3 SV=1
MM1 pKa = 7.62AFRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84VIRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84APPRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ATIRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84LRR27 pKa = 11.84KK28 pKa = 8.17KK29 pKa = 7.23TFRR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.26TGNFTLKK41 pKa = 9.94VRR43 pKa = 11.84KK44 pKa = 7.58TAVLAIDD51 pKa = 3.4SDD53 pKa = 3.25KK54 pKa = 11.3GYY56 pKa = 10.16SYY58 pKa = 11.61NVAPTLNDD66 pKa = 3.25YY67 pKa = 11.21EE68 pKa = 4.26EE69 pKa = 4.46AKK71 pKa = 10.53PYY73 pKa = 10.17IPYY76 pKa = 10.15FEE78 pKa = 5.6SYY80 pKa = 10.47RR81 pKa = 11.84IWNCSVKK88 pKa = 9.12ITPLFNVASPGEE100 pKa = 4.16AVPRR104 pKa = 11.84YY105 pKa = 9.83YY106 pKa = 10.63SAPWHH111 pKa = 6.06RR112 pKa = 11.84AGPAALSTNSILSLDD127 pKa = 3.56RR128 pKa = 11.84AKK130 pKa = 10.81SHH132 pKa = 6.64NGTSGSFRR140 pKa = 11.84RR141 pKa = 11.84YY142 pKa = 7.53TPCILTDD149 pKa = 3.39TSVAGTPGQKK159 pKa = 9.62LGKK162 pKa = 9.05MDD164 pKa = 3.94WRR166 pKa = 11.84PKK168 pKa = 9.9IALQVSSTAIPHH180 pKa = 5.19YY181 pKa = 9.82CALYY185 pKa = 9.66HH186 pKa = 6.29WSIDD190 pKa = 3.63QLQGSQITQRR200 pKa = 11.84QYY202 pKa = 10.86EE203 pKa = 4.21IEE205 pKa = 4.13IMSTITFYY213 pKa = 9.05TQKK216 pKa = 10.79SFAGG220 pKa = 3.62

Molecular weight: 25.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

497

220

277

248.5

28.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.237 ± 1.578

2.414 ± 0.691

4.427 ± 0.819

5.433 ± 1.779

4.628 ± 0.652

7.042 ± 1.343

1.61 ± 0.137

6.237 ± 0.382

6.439 ± 0.348

6.036 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.805 ± 0.368

3.823 ± 0.422

4.829 ± 1.009

3.823 ± 0.123

9.859 ± 1.288

6.64 ± 1.313

6.841 ± 0.882

4.628 ± 0.353

2.213 ± 0.26

6.036 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski