Amphibacillus marinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Amphibacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8HDV2|A0A1H8HDV2_9BACI Competence protein ComGD OS=Amphibacillus marinus OX=872970 GN=SAMN04488134_101322 PE=4 SV=1
MM1 pKa = 8.3DD2 pKa = 4.72SLRR5 pKa = 11.84KK6 pKa = 9.78RR7 pKa = 11.84GVYY10 pKa = 10.18SLAGLFLLFVLVACGGNDD28 pKa = 3.34GTATNDD34 pKa = 3.04SSGEE38 pKa = 4.02GGEE41 pKa = 4.26SGEE44 pKa = 4.08TTLINEE50 pKa = 4.32ITIMAPTFEE59 pKa = 4.54TTAPPADD66 pKa = 4.24NEE68 pKa = 3.99WEE70 pKa = 4.14QAVEE74 pKa = 4.01AFTGRR79 pKa = 11.84QLTINWVPNVSYY91 pKa = 10.41EE92 pKa = 4.0DD93 pKa = 3.58RR94 pKa = 11.84MNVTLASTDD103 pKa = 3.25IPHH106 pKa = 6.29VMVIQGKK113 pKa = 7.32TPGFLNSAEE122 pKa = 4.66AGAFWEE128 pKa = 4.55LSDD131 pKa = 4.63YY132 pKa = 11.34LDD134 pKa = 4.12DD135 pKa = 5.0YY136 pKa = 11.57EE137 pKa = 5.72YY138 pKa = 11.17LSQYY142 pKa = 11.0NEE144 pKa = 4.77DD145 pKa = 3.48ILRR148 pKa = 11.84NASVNGNVYY157 pKa = 10.44GLYY160 pKa = 9.94RR161 pKa = 11.84GRR163 pKa = 11.84DD164 pKa = 3.33IMRR167 pKa = 11.84STAIVRR173 pKa = 11.84KK174 pKa = 9.83DD175 pKa = 2.91WLDD178 pKa = 3.48NLGLDD183 pKa = 3.76VPEE186 pKa = 5.29TIDD189 pKa = 4.39DD190 pKa = 4.25LYY192 pKa = 11.49EE193 pKa = 3.75VLQAFTHH200 pKa = 7.24DD201 pKa = 4.44DD202 pKa = 3.66PDD204 pKa = 5.15GNGADD209 pKa = 3.97DD210 pKa = 4.09TVGLIIPTWYY220 pKa = 10.68GSLDD224 pKa = 3.52TLSIWFGAPNVWGYY238 pKa = 11.52EE239 pKa = 3.88NGEE242 pKa = 4.23VVPAFTTDD250 pKa = 2.76AYY252 pKa = 9.92KK253 pKa = 10.97ASLEE257 pKa = 4.07FVKK260 pKa = 10.82KK261 pKa = 10.39IVDD264 pKa = 3.65EE265 pKa = 4.45GLVNPDD271 pKa = 3.59FTTLSPDD278 pKa = 3.11DD279 pKa = 3.95WNNAMFNGQGGVIIDD294 pKa = 4.07TYY296 pKa = 10.83SRR298 pKa = 11.84AMQINNLFVDD308 pKa = 4.38EE309 pKa = 4.81TGSTDD314 pKa = 3.56PEE316 pKa = 4.01EE317 pKa = 4.73HH318 pKa = 6.01FVEE321 pKa = 4.05ITGTIRR327 pKa = 11.84SSDD330 pKa = 3.56GEE332 pKa = 4.38EE333 pKa = 3.99YY334 pKa = 10.86GQPTDD339 pKa = 3.94GYY341 pKa = 10.57SGFLAISKK349 pKa = 9.15TSVQTEE355 pKa = 4.38EE356 pKa = 4.26EE357 pKa = 4.13LHH359 pKa = 6.11EE360 pKa = 4.21VLAFLDD366 pKa = 3.86QLSSADD372 pKa = 3.99GLNLLNHH379 pKa = 7.24GIEE382 pKa = 4.29GVNYY386 pKa = 9.44EE387 pKa = 4.73LDD389 pKa = 3.78DD390 pKa = 4.29EE391 pKa = 5.67GYY393 pKa = 10.54LIPLEE398 pKa = 4.34TPEE401 pKa = 4.07AAALKK406 pKa = 9.09PYY408 pKa = 10.93EE409 pKa = 4.26MGQISTYY416 pKa = 10.5GEE418 pKa = 4.2GMHH421 pKa = 6.88TMRR424 pKa = 11.84QDD426 pKa = 2.87GALPQKK432 pKa = 10.2RR433 pKa = 11.84YY434 pKa = 9.86QLMEE438 pKa = 4.99DD439 pKa = 3.81NEE441 pKa = 4.01AHH443 pKa = 6.11AVYY446 pKa = 10.35NAAAPLVSEE455 pKa = 4.88VYY457 pKa = 7.0TTRR460 pKa = 11.84GTQLDD465 pKa = 4.84DD466 pKa = 4.55IIADD470 pKa = 3.51ARR472 pKa = 11.84IQYY475 pKa = 9.35VAGHH479 pKa = 6.65IDD481 pKa = 3.38EE482 pKa = 5.69AGWDD486 pKa = 3.62SAIDD490 pKa = 3.34LWYY493 pKa = 10.97SSGGQEE499 pKa = 6.15LIDD502 pKa = 4.4EE503 pKa = 4.65LNEE506 pKa = 5.01LYY508 pKa = 10.52TEE510 pKa = 4.38MNN512 pKa = 3.48

Molecular weight: 56.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8PIX5|A0A1H8PIX5_9BACI 50S ribosomal protein L9 OS=Amphibacillus marinus OX=872970 GN=rplI PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84HH29 pKa = 3.69VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.73KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3270

0

3270

988883

39

2731

302.4

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.444 ± 0.047

0.67 ± 0.012

5.415 ± 0.036

6.978 ± 0.045

4.532 ± 0.034

6.445 ± 0.039

2.19 ± 0.02

8.069 ± 0.035

5.798 ± 0.042

10.182 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.475 ± 0.019

4.624 ± 0.029

3.421 ± 0.024

4.711 ± 0.033

4.13 ± 0.028

5.813 ± 0.031

5.579 ± 0.033

6.825 ± 0.032

0.988 ± 0.014

3.709 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski