Miniopterus schreibersii polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S7J015|A0A1S7J015_9POLY Minor capsid protein VP2 OS=Miniopterus schreibersii polyomavirus 2 OX=1904409 PE=3 SV=1
MM1 pKa = 7.44GGVLSSVVEE10 pKa = 4.55MITMAVEE17 pKa = 3.9LSAATGISMDD27 pKa = 3.72ALLTGEE33 pKa = 4.68ALAALEE39 pKa = 4.57TEE41 pKa = 4.69VSSVMTIQGISSLEE55 pKa = 3.81ALAQLGWSAEE65 pKa = 4.17QFSNMAFISTSFTQAIGYY83 pKa = 9.53GVMFQTVTGISGLVQAGIRR102 pKa = 11.84LGFNVAAASRR112 pKa = 11.84RR113 pKa = 11.84VEE115 pKa = 3.95EE116 pKa = 4.32AQLEE120 pKa = 4.36RR121 pKa = 11.84SFGRR125 pKa = 11.84IVEE128 pKa = 4.17ALHH131 pKa = 6.69VNLSHH136 pKa = 7.34QIDD139 pKa = 3.95PLRR142 pKa = 11.84WCASLHH148 pKa = 6.41DD149 pKa = 4.82EE150 pKa = 4.57YY151 pKa = 10.6PAEE154 pKa = 3.97IHH156 pKa = 6.21QLSVQNRR163 pKa = 11.84VQFAEE168 pKa = 4.03LLKK171 pKa = 10.16VGRR174 pKa = 11.84WVHH177 pKa = 5.13QAHH180 pKa = 6.38FTTNPLYY187 pKa = 10.5EE188 pKa = 4.5SGNIISKK195 pKa = 10.26FDD197 pKa = 3.72PPGGAFQDD205 pKa = 3.95VTPDD209 pKa = 2.96WLVNLILRR217 pKa = 11.84LHH219 pKa = 7.11DD220 pKa = 3.6GTAEE224 pKa = 3.89EE225 pKa = 5.78AIPFCNRR232 pKa = 11.84VSSS235 pKa = 3.7

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S7J013|A0A1S7J013_9POLY Minor capsid protein VP2 OS=Miniopterus schreibersii polyomavirus 2 OX=1904409 PE=3 SV=1
MM1 pKa = 7.94DD2 pKa = 4.96PVRR5 pKa = 11.84SEE7 pKa = 4.1SDD9 pKa = 3.36TLRR12 pKa = 11.84GPPEE16 pKa = 4.1YY17 pKa = 10.78LPMKK21 pKa = 10.38GLDD24 pKa = 3.24PSQIYY29 pKa = 10.28IAMNPVTPPHH39 pKa = 6.25QKK41 pKa = 10.16RR42 pKa = 11.84PPPQAKK48 pKa = 9.42PPPQDD53 pKa = 3.8TIPTPLILDD62 pKa = 3.82VDD64 pKa = 4.43SLPPPLPPKK73 pKa = 9.52TRR75 pKa = 11.84SPPRR79 pKa = 11.84RR80 pKa = 11.84SVSLPSRR87 pKa = 11.84RR88 pKa = 11.84PPPNPLMVEE97 pKa = 4.05VEE99 pKa = 3.93NLEE102 pKa = 3.73RR103 pKa = 11.84SLRR106 pKa = 11.84QKK108 pKa = 10.51EE109 pKa = 4.02QDD111 pKa = 3.92LVTMWMDD118 pKa = 3.47LVRR121 pKa = 11.84ARR123 pKa = 11.84KK124 pKa = 7.53QASRR128 pKa = 11.84ARR130 pKa = 11.84HH131 pKa = 4.13QRR133 pKa = 11.84KK134 pKa = 7.3KK135 pKa = 10.2QKK137 pKa = 9.6WVMGLLIFLLVFLIILAMLYY157 pKa = 10.77LLIKK161 pKa = 10.33HH162 pKa = 6.74LIASS166 pKa = 4.21

Molecular weight: 19.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1874

166

714

312.3

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.564 ± 0.928

2.561 ± 0.727

4.696 ± 0.418

6.137 ± 0.539

4.216 ± 0.759

5.816 ± 1.037

2.295 ± 0.278

4.589 ± 0.626

5.176 ± 1.06

11.099 ± 0.897

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.615 ± 0.347

3.522 ± 0.386

6.403 ± 1.619

5.016 ± 0.53

5.443 ± 0.635

7.898 ± 0.731

5.55 ± 0.316

6.51 ± 0.527

1.334 ± 0.376

2.561 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski