Sewage associated gemycircularvirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus sewopo1; Sewage derived gemykibivirus 1

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161IVR5|A0A161IVR5_9VIRU RepA OS=Sewage associated gemycircularvirus 3 OX=1843761 PE=3 SV=1
MM1 pKa = 7.45TFRR4 pKa = 11.84FAAKK8 pKa = 10.31YY9 pKa = 10.64GLLTYY14 pKa = 10.56AQIGDD19 pKa = 3.65RR20 pKa = 11.84DD21 pKa = 3.79VEE23 pKa = 4.24EE24 pKa = 4.58FGWRR28 pKa = 11.84VSDD31 pKa = 3.48MLGSLGAEE39 pKa = 3.96CIVGRR44 pKa = 11.84EE45 pKa = 3.97SHH47 pKa = 6.97ADD49 pKa = 3.29GGLHH53 pKa = 5.1IHH55 pKa = 6.74AFFMFEE61 pKa = 4.81RR62 pKa = 11.84KK63 pKa = 8.84FQSRR67 pKa = 11.84NVRR70 pKa = 11.84VFDD73 pKa = 4.43MDD75 pKa = 3.54GCHH78 pKa = 6.22PNIVRR83 pKa = 11.84GYY85 pKa = 7.79STPEE89 pKa = 3.72DD90 pKa = 3.56GARR93 pKa = 11.84YY94 pKa = 9.33AIKK97 pKa = 10.44EE98 pKa = 3.95GDD100 pKa = 3.98VIAGGLDD107 pKa = 3.41VDD109 pKa = 4.37SLGSSVAGSKK119 pKa = 7.63TVWAQIILAEE129 pKa = 4.26SRR131 pKa = 11.84DD132 pKa = 3.92DD133 pKa = 4.9FFAACAEE140 pKa = 4.11LAPRR144 pKa = 11.84ALLCSFTSLRR154 pKa = 11.84CYY156 pKa = 10.98ADD158 pKa = 2.7WKK160 pKa = 10.01YY161 pKa = 11.04RR162 pKa = 11.84EE163 pKa = 4.58DD164 pKa = 3.75PAPYY168 pKa = 9.65RR169 pKa = 11.84HH170 pKa = 6.68PEE172 pKa = 4.08DD173 pKa = 3.74VSFDD177 pKa = 3.39TSRR180 pKa = 11.84FPEE183 pKa = 4.14LDD185 pKa = 2.56RR186 pKa = 11.84WVQDD190 pKa = 3.46SLRR193 pKa = 11.84GAQRR197 pKa = 11.84GRR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84SLILYY206 pKa = 9.32GPTKK210 pKa = 10.4LGKK213 pKa = 7.53TLWARR218 pKa = 11.84SLGNHH223 pKa = 7.12AYY225 pKa = 10.38FGGLFSMDD233 pKa = 3.49EE234 pKa = 4.55SIDD237 pKa = 3.78DD238 pKa = 2.96VDD240 pKa = 3.91YY241 pKa = 11.58AVFDD245 pKa = 4.3DD246 pKa = 4.09MQGGLKK252 pKa = 10.09FFHH255 pKa = 7.11SYY257 pKa = 10.7KK258 pKa = 10.25FWLGAQSQFYY268 pKa = 8.76VTEE271 pKa = 4.22KK272 pKa = 10.81YY273 pKa = 9.41KK274 pKa = 10.96GKK276 pKa = 10.51RR277 pKa = 11.84LVHH280 pKa = 6.05WGKK283 pKa = 9.71PCIYY287 pKa = 9.84LYY289 pKa = 10.87NHH291 pKa = 6.64NPLCDD296 pKa = 3.41EE297 pKa = 4.74GADD300 pKa = 4.1HH301 pKa = 7.86DD302 pKa = 4.56WLLGNCDD309 pKa = 3.68IVGLDD314 pKa = 4.13ADD316 pKa = 4.81DD317 pKa = 4.32SLLVPEE323 pKa = 4.95EE324 pKa = 4.32GSLGGEE330 pKa = 4.03RR331 pKa = 4.37

Molecular weight: 37.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161J5N3|A0A161J5N3_9VIRU Capsid protein OS=Sewage associated gemycircularvirus 3 OX=1843761 PE=4 SV=1
MM1 pKa = 7.4VYY3 pKa = 10.29RR4 pKa = 11.84RR5 pKa = 11.84LSRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 8.1SAYY13 pKa = 9.2RR14 pKa = 11.84PRR16 pKa = 11.84SAKK19 pKa = 9.75SRR21 pKa = 11.84YY22 pKa = 7.34GRR24 pKa = 11.84KK25 pKa = 7.78RR26 pKa = 11.84TRR28 pKa = 11.84STRR31 pKa = 11.84VRR33 pKa = 11.84VSRR36 pKa = 11.84RR37 pKa = 11.84MRR39 pKa = 11.84PMSTRR44 pKa = 11.84SLLNRR49 pKa = 11.84TSQKK53 pKa = 10.39KK54 pKa = 8.85RR55 pKa = 11.84DD56 pKa = 3.67NMLSYY61 pKa = 11.09TNTTAADD68 pKa = 3.86PFSTVYY74 pKa = 10.47SATGAVMRR82 pKa = 11.84FPVGQINPGEE92 pKa = 3.96EE93 pKa = 4.56FFYY96 pKa = 10.95VWNATARR103 pKa = 11.84PGEE106 pKa = 4.09TSEE109 pKa = 4.12GQRR112 pKa = 11.84GSKK115 pKa = 9.73IDD117 pKa = 3.35TSLRR121 pKa = 11.84TSSTIYY127 pKa = 10.87AKK129 pKa = 10.39GLRR132 pKa = 11.84EE133 pKa = 4.36KK134 pKa = 9.95ITVEE138 pKa = 4.44TNNSAPWEE146 pKa = 3.86WRR148 pKa = 11.84RR149 pKa = 11.84ICFTSKK155 pKa = 11.11DD156 pKa = 3.86DD157 pKa = 4.07FGEE160 pKa = 4.4RR161 pKa = 11.84DD162 pKa = 3.46PASSTFFRR170 pKa = 11.84QTSSGMVRR178 pKa = 11.84MLSALSTGIYY188 pKa = 10.09LQDD191 pKa = 3.53EE192 pKa = 4.67LFEE195 pKa = 4.39GARR198 pKa = 11.84NVDD201 pKa = 3.21WLSVFTAPLSRR212 pKa = 11.84KK213 pKa = 9.23NFSIKK218 pKa = 9.97YY219 pKa = 9.43DD220 pKa = 3.31RR221 pKa = 11.84TRR223 pKa = 11.84TIRR226 pKa = 11.84STNNSGTIRR235 pKa = 11.84NYY237 pKa = 10.79KK238 pKa = 8.98LWHH241 pKa = 5.96PMEE244 pKa = 5.71HH245 pKa = 5.94NLAYY249 pKa = 10.28QEE251 pKa = 4.22EE252 pKa = 4.64QVGEE256 pKa = 4.29SMTDD260 pKa = 3.07QSVSVTGRR268 pKa = 11.84VGMGNYY274 pKa = 9.59YY275 pKa = 10.35VIDD278 pKa = 3.74MFRR281 pKa = 11.84KK282 pKa = 9.93HH283 pKa = 6.33GNNDD287 pKa = 3.17NDD289 pKa = 3.72STLTFTPEE297 pKa = 3.46ATFFWHH303 pKa = 6.68EE304 pKa = 4.05KK305 pKa = 9.79

Molecular weight: 35.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

877

241

331

292.3

33.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.184 ± 0.877

1.71 ± 0.603

7.412 ± 1.374

5.245 ± 0.111

5.587 ± 0.295

8.438 ± 1.128

2.395 ± 0.471

3.649 ± 0.209

3.763 ± 0.512

7.526 ± 1.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.48

3.079 ± 1.089

3.649 ± 0.202

2.395 ± 0.132

9.692 ± 1.168

9.008 ± 1.053

5.245 ± 2.145

5.473 ± 0.137

2.052 ± 0.186

3.991 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski