Propionibacteriaceae bacterium NML 150081

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; unclassified Propionibacteriaceae

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255EJC1|A0A255EJC1_9ACTN Uncharacterized protein OS=Propionibacteriaceae bacterium NML 150081 OX=2016505 GN=CGZ91_11650 PE=4 SV=1
MM1 pKa = 7.33TSKK4 pKa = 10.58FDD6 pKa = 4.61DD7 pKa = 4.12MDD9 pKa = 3.17WHH11 pKa = 7.24LGGDD15 pKa = 4.48DD16 pKa = 4.9FPDD19 pKa = 4.35DD20 pKa = 4.29LPEE23 pKa = 4.0EE24 pKa = 4.3AAATHH29 pKa = 6.03IGMYY33 pKa = 7.4FTWLVEE39 pKa = 4.05RR40 pKa = 11.84GLVGEE45 pKa = 4.34DD46 pKa = 3.12LVRR49 pKa = 11.84EE50 pKa = 4.21HH51 pKa = 7.01GAALDD56 pKa = 3.78QVRR59 pKa = 11.84SGQLRR64 pKa = 11.84GSGFIVDD71 pKa = 3.67QCDD74 pKa = 3.65GQLLAQYY81 pKa = 8.36LTEE84 pKa = 4.12VGAAFTEE91 pKa = 4.43HH92 pKa = 7.05VYY94 pKa = 11.09DD95 pKa = 4.87SYY97 pKa = 11.89LEE99 pKa = 4.76EE100 pKa = 4.25YY101 pKa = 10.59GNWGDD106 pKa = 3.78PNVNIYY112 pKa = 10.67ALPDD116 pKa = 3.35NDD118 pKa = 3.38EE119 pKa = 4.7TYY121 pKa = 11.11SEE123 pKa = 4.33VQDD126 pKa = 4.43LLDD129 pKa = 3.74SRR131 pKa = 11.84FAEE134 pKa = 4.22WDD136 pKa = 3.48PSS138 pKa = 3.47

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255EFH9|A0A255EFH9_9ACTN Ribonuclease P protein component OS=Propionibacteriaceae bacterium NML 150081 OX=2016505 GN=rnpA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIMSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.85GRR40 pKa = 11.84AKK42 pKa = 10.71LAGG45 pKa = 3.63

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2661

0

2661

906985

33

2359

340.8

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.475 ± 0.064

0.678 ± 0.012

6.277 ± 0.041

6.024 ± 0.043

2.742 ± 0.025

9.014 ± 0.045

2.212 ± 0.025

4.181 ± 0.032

1.975 ± 0.031

9.983 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.02 ± 0.021

2.09 ± 0.027

5.819 ± 0.045

3.666 ± 0.033

7.124 ± 0.05

5.441 ± 0.027

6.038 ± 0.039

8.534 ± 0.042

1.74 ± 0.022

1.969 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski