Yongsan tombus-like virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; unclassified Tombusviridae

Average proteome isoelectric point is 8.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A385FQG9|A0A385FQG9_9TOMB Uncharacterized protein OS=Yongsan tombus-like virus 1 OX=2315813 PE=4 SV=1
MM1 pKa = 7.41SLNDD5 pKa = 3.46KK6 pKa = 9.87VLPGSCLSAGACDD19 pKa = 4.24HH20 pKa = 5.37VCKK23 pKa = 10.49RR24 pKa = 11.84YY25 pKa = 6.35TQQMFTIQGFTSEE38 pKa = 4.12VWTHH42 pKa = 6.14GSCVCNEE49 pKa = 3.94VIALKK54 pKa = 10.37NRR56 pKa = 11.84HH57 pKa = 5.21QLCDD61 pKa = 3.4GSAYY65 pKa = 10.81NSTVDD70 pKa = 4.02LRR72 pKa = 11.84RR73 pKa = 11.84PLRR76 pKa = 11.84KK77 pKa = 9.78LITNLEE83 pKa = 4.29PCSEE87 pKa = 4.25QTVIAHH93 pKa = 7.28ADD95 pKa = 3.5GSRR98 pKa = 11.84KK99 pKa = 9.7KK100 pKa = 10.46LLQRR104 pKa = 11.84AADD107 pKa = 3.68SLSTHH112 pKa = 5.62GLEE115 pKa = 6.11HH116 pKa = 6.74KK117 pKa = 10.28DD118 pKa = 2.94AWVKK122 pKa = 10.38MFLKK126 pKa = 10.25DD127 pKa = 4.24DD128 pKa = 4.11KK129 pKa = 11.43YY130 pKa = 10.51HH131 pKa = 5.34TPEE134 pKa = 4.02YY135 pKa = 9.99KK136 pKa = 10.36APRR139 pKa = 11.84CIQYY143 pKa = 10.18RR144 pKa = 11.84NKK146 pKa = 10.62RR147 pKa = 11.84YY148 pKa = 9.98GLRR151 pKa = 11.84LATFLHH157 pKa = 6.98PIEE160 pKa = 4.32QRR162 pKa = 11.84VITLKK167 pKa = 11.09HH168 pKa = 5.94NGTHH172 pKa = 5.58VFAKK176 pKa = 9.07GRR178 pKa = 11.84NMKK181 pKa = 9.93QRR183 pKa = 11.84GRR185 pKa = 11.84DD186 pKa = 3.19IAAKK190 pKa = 10.39LLPNWVAVSMDD201 pKa = 3.08HH202 pKa = 7.32SKK204 pKa = 10.78FDD206 pKa = 3.49SHH208 pKa = 8.04VNEE211 pKa = 3.89HH212 pKa = 6.67LLRR215 pKa = 11.84LEE217 pKa = 3.5HH218 pKa = 6.94WYY220 pKa = 10.98YY221 pKa = 10.11NQCINDD227 pKa = 4.41PEE229 pKa = 4.66LKK231 pKa = 9.85WLLEE235 pKa = 3.97CQLRR239 pKa = 11.84NKK241 pKa = 10.33GFTKK245 pKa = 10.75NNTRR249 pKa = 11.84YY250 pKa = 5.23TTRR253 pKa = 11.84ATRR256 pKa = 11.84MSGDD260 pKa = 3.33QNTGLGNCIINYY272 pKa = 10.34AMTKK276 pKa = 10.88AMLDD280 pKa = 3.56HH281 pKa = 7.29LDD283 pKa = 3.84IPHH286 pKa = 6.82NLYY289 pKa = 10.06IDD291 pKa = 3.99GDD293 pKa = 4.02DD294 pKa = 4.51FIVFVHH300 pKa = 6.87RR301 pKa = 11.84KK302 pKa = 8.23HH303 pKa = 6.37KK304 pKa = 10.45HH305 pKa = 5.78LIDD308 pKa = 4.4PNWYY312 pKa = 9.1KK313 pKa = 10.65QFGMKK318 pKa = 7.62TTTDD322 pKa = 3.26QITDD326 pKa = 3.62VLEE329 pKa = 5.48HH330 pKa = 7.39IDD332 pKa = 3.8FCQCRR337 pKa = 11.84PVYY340 pKa = 10.9DD341 pKa = 3.96GDD343 pKa = 4.0SYY345 pKa = 11.56TLVRR349 pKa = 11.84NPARR353 pKa = 11.84LLARR357 pKa = 11.84LPWLVGPINGRR368 pKa = 11.84SPWNIVASAAQCEE381 pKa = 4.11ISLGYY386 pKa = 10.06GLPIGQYY393 pKa = 8.61VGHH396 pKa = 6.44NVFLYY401 pKa = 10.7ASNKK405 pKa = 9.28GGRR408 pKa = 11.84FKK410 pKa = 11.27SNCVAEE416 pKa = 4.66WRR418 pKa = 11.84HH419 pKa = 5.18KK420 pKa = 8.27MEE422 pKa = 4.7RR423 pKa = 11.84MKK425 pKa = 10.84AGNLKK430 pKa = 10.22PVDD433 pKa = 3.77CAPGVRR439 pKa = 11.84ASYY442 pKa = 9.63EE443 pKa = 4.13RR444 pKa = 11.84AWGISIPQQYY454 pKa = 9.78IIEE457 pKa = 4.62HH458 pKa = 5.85ATIAPPFEE466 pKa = 4.69EE467 pKa = 4.98EE468 pKa = 3.76WDD470 pKa = 3.5HH471 pKa = 6.74TPFDD475 pKa = 4.04RR476 pKa = 11.84KK477 pKa = 8.86TYY479 pKa = 10.53

Molecular weight:
55.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A385FQH1|A0A385FQH1_9TOMB Uncharacterized protein OS=Yongsan tombus-like virus 1 OX=2315813 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84ALQVEE7 pKa = 4.48LQHH10 pKa = 6.86LRR12 pKa = 11.84RR13 pKa = 11.84ANSALQARR21 pKa = 11.84VRR23 pKa = 11.84DD24 pKa = 3.88LEE26 pKa = 4.07GLITRR31 pKa = 11.84NTSAPLGGSGPITTIPRR48 pKa = 11.84GSRR51 pKa = 11.84RR52 pKa = 11.84STLVRR57 pKa = 11.84ASGVASTPNLNSSPEE72 pKa = 4.0PRR74 pKa = 11.84SQRR77 pKa = 11.84PVPHH81 pKa = 6.71VNTTPPVSRR90 pKa = 11.84RR91 pKa = 11.84QSSTSPRR98 pKa = 11.84VGTPSRR104 pKa = 11.84PQSVTALTRR113 pKa = 11.84PVTPASAGQAAEE125 pKa = 4.16HH126 pKa = 6.13STVSTQLSSGPALSPAPEE144 pKa = 4.11TTASKK149 pKa = 9.68TVVSHH154 pKa = 6.92PYY156 pKa = 10.19AGAILEE162 pKa = 4.09ATRR165 pKa = 11.84AKK167 pKa = 10.96GEE169 pKa = 4.07LAHH172 pKa = 7.13DD173 pKa = 3.76EE174 pKa = 4.39RR175 pKa = 11.84PVYY178 pKa = 10.39VADD181 pKa = 3.72HH182 pKa = 6.78AIDD185 pKa = 3.56RR186 pKa = 11.84SLRR189 pKa = 11.84EE190 pKa = 3.84SVGITRR196 pKa = 11.84RR197 pKa = 11.84AVNKK201 pKa = 6.42MTAVDD206 pKa = 3.72EE207 pKa = 4.41EE208 pKa = 4.72EE209 pKa = 4.28PKK211 pKa = 11.21GMWRR215 pKa = 11.84YY216 pKa = 8.98LPKK219 pKa = 10.26VRR221 pKa = 11.84SPALRR226 pKa = 11.84ADD228 pKa = 3.42KK229 pKa = 11.05YY230 pKa = 11.73LLFFLLNKK238 pKa = 9.92FAFIEE243 pKa = 4.19RR244 pKa = 11.84SPDD247 pKa = 2.62TMRR250 pKa = 11.84RR251 pKa = 11.84MWEE254 pKa = 3.89SLNQVMRR261 pKa = 11.84DD262 pKa = 2.94FDD264 pKa = 3.9TRR266 pKa = 11.84NNTIEE271 pKa = 4.27EE272 pKa = 4.35IYY274 pKa = 10.41LLKK277 pKa = 10.5HH278 pKa = 4.54QAVRR282 pKa = 11.84AAMVPPAEE290 pKa = 4.27EE291 pKa = 3.69LRR293 pKa = 11.84TRR295 pKa = 11.84KK296 pKa = 9.48LLQEE300 pKa = 4.39APNLDD305 pKa = 3.2KK306 pKa = 10.87HH307 pKa = 7.15AKK309 pKa = 7.03FTKK312 pKa = 10.08DD313 pKa = 2.53GHH315 pKa = 6.69LGHH318 pKa = 7.07KK319 pKa = 10.33LEE321 pKa = 5.29ASLSTIGNLFQPTKK335 pKa = 10.72SLPTKK340 pKa = 10.28KK341 pKa = 10.5

Molecular weight:
37.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1228

341

479

409.3

45.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.062 ± 0.909

1.547 ± 0.927

3.746 ± 1.058

4.56 ± 0.488

2.85 ± 0.3

5.537 ± 0.35

3.094 ± 0.998

4.642 ± 0.66

4.886 ± 0.818

9.365 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.2

4.479 ± 0.594

6.84 ± 1.134

4.56 ± 0.611

7.166 ± 1.061

7.573 ± 1.383

8.143 ± 1.288

6.596 ± 0.756

1.466 ± 0.453

2.932 ± 0.705

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski