Staphylococcus phage B166

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus B166

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3T8I4|A0A0E3T8I4_9CAUD Uncharacterized protein OS=Staphylococcus phage B166 OX=1636204 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.77EE41 pKa = 4.31ILKK44 pKa = 10.03GLPNAMQDD52 pKa = 3.36ALKK55 pKa = 10.66EE56 pKa = 4.16DD57 pKa = 3.68IYY59 pKa = 11.25LDD61 pKa = 3.66EE62 pKa = 5.2AVGIMTGQVVYY73 pKa = 10.25KK74 pKa = 10.67YY75 pKa = 10.68EE76 pKa = 3.97EE77 pKa = 4.06EE78 pKa = 4.28QEE80 pKa = 3.93NDD82 pKa = 2.92

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3T9H4|A0A0E3T9H4_9CAUD Uncharacterized protein OS=Staphylococcus phage B166 OX=1636204 PE=4 SV=1
MM1 pKa = 7.61LMAKK5 pKa = 10.19RR6 pKa = 11.84GKK8 pKa = 10.59KK9 pKa = 9.59NGKK12 pKa = 9.21QSTSRR17 pKa = 11.84TAKK20 pKa = 10.04LASKK24 pKa = 9.57VLRR27 pKa = 11.84DD28 pKa = 3.41KK29 pKa = 11.31RR30 pKa = 11.84SGKK33 pKa = 9.29KK34 pKa = 9.87AKK36 pKa = 10.24SLAGSVLAQSS46 pKa = 3.91

Molecular weight: 4.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13470

46

961

210.5

24.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.523 ± 0.381

0.438 ± 0.074

6.488 ± 0.268

7.506 ± 0.45

4.462 ± 0.218

5.813 ± 0.323

1.782 ± 0.139

7.528 ± 0.254

9.198 ± 0.388

7.55 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.199

6.652 ± 0.312

2.858 ± 0.165

3.86 ± 0.246

4.209 ± 0.242

6.088 ± 0.288

5.969 ± 0.258

5.999 ± 0.34

1.188 ± 0.158

4.209 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski