Hespellia stercorisuis DSM 15480

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Hespellia; Hespellia stercorisuis

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6MU35|A0A1M6MU35_9FIRM L-threonine aldolase OS=Hespellia stercorisuis DSM 15480 OX=1121950 GN=SAMN02745243_01586 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.16KK3 pKa = 10.15KK4 pKa = 10.68VIAALMCAALVLGMTACGSSSDD26 pKa = 3.85SSSGSSAKK34 pKa = 10.21KK35 pKa = 9.9EE36 pKa = 4.22SGGDD40 pKa = 3.48GYY42 pKa = 11.42KK43 pKa = 9.95IGFTDD48 pKa = 3.58NYY50 pKa = 10.27NGNSYY55 pKa = 8.92HH56 pKa = 7.29QSMEE60 pKa = 3.74KK61 pKa = 10.64YY62 pKa = 7.76MQEE65 pKa = 4.18VADD68 pKa = 4.21EE69 pKa = 4.37LKK71 pKa = 10.83KK72 pKa = 11.21SGDD75 pKa = 3.24ISEE78 pKa = 4.28LTIAEE83 pKa = 4.36ANQDD87 pKa = 3.28AATQISQIEE96 pKa = 4.31DD97 pKa = 3.47FVMQGYY103 pKa = 10.05DD104 pKa = 4.08LIVVDD109 pKa = 4.61PCSTSSLNSAVQEE122 pKa = 4.26ACDD125 pKa = 3.49AGIPVLVINDD135 pKa = 3.75GPIDD139 pKa = 3.79YY140 pKa = 10.79EE141 pKa = 4.03NDD143 pKa = 2.98LVYY146 pKa = 10.76QLNFDD151 pKa = 4.19FTDD154 pKa = 3.8LAYY157 pKa = 9.55TLTKK161 pKa = 9.89EE162 pKa = 4.0VCEE165 pKa = 4.07QIGGKK170 pKa = 10.39GSIIEE175 pKa = 4.1LRR177 pKa = 11.84GTAGTTCDD185 pKa = 3.44TQMHH189 pKa = 6.33EE190 pKa = 4.6GVEE193 pKa = 4.38KK194 pKa = 10.61ALKK197 pKa = 9.75EE198 pKa = 4.07YY199 pKa = 10.02PDD201 pKa = 3.49VKK203 pKa = 10.13IASEE207 pKa = 5.03IYY209 pKa = 10.12TDD211 pKa = 3.35WTGSKK216 pKa = 10.1AQSEE220 pKa = 4.77LNSVLPTLDD229 pKa = 3.57KK230 pKa = 11.39VDD232 pKa = 3.88GLVTQGGDD240 pKa = 2.67AYY242 pKa = 10.79AAVQAFTSAGYY253 pKa = 10.21SEE255 pKa = 5.37LPVIAGDD262 pKa = 3.63NRR264 pKa = 11.84GSFLSWWAKK273 pKa = 9.88DD274 pKa = 3.37APEE277 pKa = 4.78GYY279 pKa = 8.15EE280 pKa = 4.3TISGACNPWDD290 pKa = 3.91GGMAMYY296 pKa = 10.24IAVDD300 pKa = 3.5ILNGEE305 pKa = 4.19DD306 pKa = 3.14VANNMNCPFGYY317 pKa = 10.56VKK319 pKa = 10.78ADD321 pKa = 3.89DD322 pKa = 3.78VAQYY326 pKa = 11.76ADD328 pKa = 3.39MAEE331 pKa = 4.59DD332 pKa = 4.13EE333 pKa = 4.7VASTSYY339 pKa = 11.26DD340 pKa = 2.92WDD342 pKa = 4.26TIKK345 pKa = 11.0SDD347 pKa = 3.74IEE349 pKa = 3.96PGKK352 pKa = 10.75

Molecular weight: 37.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6RAJ8|A0A1M6RAJ8_9FIRM Endonuclease MutS2 OS=Hespellia stercorisuis DSM 15480 OX=1121950 GN=mutS2 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSPGGRR28 pKa = 11.84KK29 pKa = 8.84VLQARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4054

0

4054

1281107

39

4450

316.0

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.59 ± 0.046

1.456 ± 0.017

5.713 ± 0.036

7.76 ± 0.052

3.912 ± 0.024

7.069 ± 0.044

1.73 ± 0.018

7.216 ± 0.038

6.901 ± 0.039

8.703 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.158 ± 0.024

4.366 ± 0.03

3.154 ± 0.02

3.438 ± 0.024

4.382 ± 0.034

5.807 ± 0.033

5.697 ± 0.051

7.017 ± 0.034

0.887 ± 0.013

4.044 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski