Gordonia phage Blueberry

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166Y3J0|A0A166Y3J0_9CAUD Uncharacterized protein OS=Gordonia phage Blueberry OX=1838062 GN=35 PE=4 SV=1
MM1 pKa = 7.15MANDD5 pKa = 4.88TIFEE9 pKa = 4.28LPEE12 pKa = 4.65IPGVTFTASYY22 pKa = 10.84GSGGEE27 pKa = 4.12TGLPSNWIRR36 pKa = 11.84IVGTVEE42 pKa = 3.86NPWYY46 pKa = 10.31DD47 pKa = 3.26PTYY50 pKa = 11.17NYY52 pKa = 11.17GLDD55 pKa = 3.93PNGHH59 pKa = 5.52TEE61 pKa = 4.34ITDD64 pKa = 2.86PWKK67 pKa = 10.66RR68 pKa = 11.84HH69 pKa = 3.79TQFPEE74 pKa = 3.86VCAMGFGGPSIGLPTDD90 pKa = 3.96PPPPPVEE97 pKa = 4.39PEE99 pKa = 3.83PTPDD103 pKa = 3.89IIEE106 pKa = 4.47EE107 pKa = 4.28PTDD110 pKa = 3.47GG111 pKa = 4.67

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DCS1|A0A160DCS1_9CAUD Tail assembly chaperone OS=Gordonia phage Blueberry OX=1838062 GN=18 PE=4 SV=1
MM1 pKa = 7.3NADD4 pKa = 3.46TTEE7 pKa = 4.03DD8 pKa = 3.32RR9 pKa = 11.84VTTRR13 pKa = 11.84LRR15 pKa = 11.84PSPFTNVADD24 pKa = 4.48DD25 pKa = 3.66GRR27 pKa = 11.84SRR29 pKa = 11.84FAEE32 pKa = 3.71RR33 pKa = 11.84DD34 pKa = 3.5DD35 pKa = 5.04FRR37 pKa = 11.84DD38 pKa = 3.36LTPYY42 pKa = 10.69EE43 pKa = 3.99EE44 pKa = 5.72AILHH48 pKa = 6.09GMTKK52 pKa = 9.99PAYY55 pKa = 9.28FPGRR59 pKa = 11.84GYY61 pKa = 11.45FMVQGVYY68 pKa = 9.66QGYY71 pKa = 8.0TDD73 pKa = 4.6DD74 pKa = 4.97RR75 pKa = 11.84VITHH79 pKa = 7.36RR80 pKa = 11.84DD81 pKa = 3.01GTIEE85 pKa = 4.09VVPDD89 pKa = 3.3PRR91 pKa = 11.84IARR94 pKa = 11.84TEE96 pKa = 3.57RR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84AKK101 pKa = 10.44NKK103 pKa = 9.87AGRR106 pKa = 11.84KK107 pKa = 6.53SRR109 pKa = 11.84RR110 pKa = 11.84INRR113 pKa = 11.84IRR115 pKa = 11.84AQRR118 pKa = 3.33

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

17621

32

1829

204.9

22.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.39 ± 0.487

0.925 ± 0.141

7.054 ± 0.338

5.959 ± 0.308

2.758 ± 0.132

8.274 ± 0.314

2.196 ± 0.161

4.33 ± 0.169

3.184 ± 0.225

7.502 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.15

3.093 ± 0.178

5.879 ± 0.246

3.439 ± 0.131

7.82 ± 0.37

5.034 ± 0.141

6.804 ± 0.197

7.763 ± 0.244

2.088 ± 0.101

2.304 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski