Oceanobacillus halophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3727 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495AB14|A0A495AB14_9BACI Chromate transporter OS=Oceanobacillus halophilus OX=930130 GN=D8M06_05090 PE=3 SV=1
MM1 pKa = 6.96MVIAFVLAACSGSNTSDD18 pKa = 2.72TGEE21 pKa = 3.83AVQEE25 pKa = 4.37GNNPTEE31 pKa = 4.47SANSQPGTEE40 pKa = 3.98NVLRR44 pKa = 11.84IAMSTEE50 pKa = 3.57VDD52 pKa = 3.41NLDD55 pKa = 4.18PYY57 pKa = 10.93ISAATDD63 pKa = 3.53TQSMMDD69 pKa = 3.54NVFDD73 pKa = 4.43GLLDD77 pKa = 3.62TDD79 pKa = 3.91EE80 pKa = 4.64SGKK83 pKa = 10.24LVPAIAEE90 pKa = 4.28DD91 pKa = 4.07YY92 pKa = 10.18EE93 pKa = 4.32ISEE96 pKa = 5.33DD97 pKa = 3.41GLTYY101 pKa = 10.16TFQLKK106 pKa = 10.31EE107 pKa = 4.44GILFHH112 pKa = 7.44DD113 pKa = 4.84GSEE116 pKa = 4.51LISEE120 pKa = 4.46DD121 pKa = 3.06VVYY124 pKa = 10.53SYY126 pKa = 11.61EE127 pKa = 4.11RR128 pKa = 11.84LSGLNGEE135 pKa = 5.0EE136 pKa = 4.13PLSSQFAGIEE146 pKa = 4.13NIEE149 pKa = 4.23APSDD153 pKa = 3.85YY154 pKa = 10.95EE155 pKa = 4.17VVITLKK161 pKa = 10.59EE162 pKa = 3.97NNSAFLAANIRR173 pKa = 11.84PILPAGYY180 pKa = 9.16EE181 pKa = 4.1EE182 pKa = 5.11QSTSPIGAGPFKK194 pKa = 10.78FEE196 pKa = 4.61EE197 pKa = 4.42YY198 pKa = 10.19QAGQQLTLVKK208 pKa = 10.95NEE210 pKa = 4.4DD211 pKa = 3.99YY212 pKa = 11.58YY213 pKa = 11.18MQDD216 pKa = 3.03KK217 pKa = 10.62VPPFDD222 pKa = 3.29KK223 pKa = 10.93VQFIVMPDD231 pKa = 3.45PEE233 pKa = 4.56SAVLAMQAGEE243 pKa = 4.07IDD245 pKa = 4.68VIPGVTPQGMMQLGEE260 pKa = 4.35SVNTVSGPQNMVQLMALNNEE280 pKa = 4.26VEE282 pKa = 4.36PLDD285 pKa = 4.01DD286 pKa = 3.93VNVRR290 pKa = 11.84KK291 pKa = 10.03AINLAIDD298 pKa = 3.38KK299 pKa = 11.0DD300 pKa = 4.12MIIEE304 pKa = 4.31TVADD308 pKa = 3.75GMGTKK313 pKa = 10.32LGSNFSPAMDD323 pKa = 4.52FYY325 pKa = 11.75YY326 pKa = 10.56EE327 pKa = 4.72AGLEE331 pKa = 4.7DD332 pKa = 4.59YY333 pKa = 8.99YY334 pKa = 11.37QPNIEE339 pKa = 4.09EE340 pKa = 4.36AKK342 pKa = 10.86ALLADD347 pKa = 4.25AGYY350 pKa = 11.43ADD352 pKa = 4.97GFDD355 pKa = 4.69LEE357 pKa = 4.66LTVPSDD363 pKa = 3.67YY364 pKa = 10.78QFHH367 pKa = 6.93VDD369 pKa = 3.22TAQVVVEE376 pKa = 4.02QLSQIGINVEE386 pKa = 3.49IKK388 pKa = 10.67LIEE391 pKa = 4.23FSSWLEE397 pKa = 3.7TVYY400 pKa = 11.01QDD402 pKa = 3.46AQYY405 pKa = 10.95EE406 pKa = 4.39STIVSFTGKK415 pKa = 10.04LDD417 pKa = 3.77PYY419 pKa = 9.44EE420 pKa = 3.94VLVRR424 pKa = 11.84YY425 pKa = 10.09ISDD428 pKa = 3.62YY429 pKa = 10.73QSNFVNYY436 pKa = 10.21NNPEE440 pKa = 3.68YY441 pKa = 10.69DD442 pKa = 3.52ALIEE446 pKa = 4.01QAVASTDD453 pKa = 3.62EE454 pKa = 4.61NEE456 pKa = 4.07MADD459 pKa = 4.58LYY461 pKa = 11.19KK462 pKa = 10.28QAQRR466 pKa = 11.84LLTEE470 pKa = 4.81DD471 pKa = 3.45AASVYY476 pKa = 10.7IMDD479 pKa = 5.14PDD481 pKa = 2.98RR482 pKa = 11.84TYY484 pKa = 11.77ALRR487 pKa = 11.84SGLEE491 pKa = 3.75GWKK494 pKa = 9.27MYY496 pKa = 9.85PIQKK500 pKa = 10.05FNLEE504 pKa = 4.58DD505 pKa = 3.41IRR507 pKa = 11.84VTEE510 pKa = 4.06

Molecular weight: 56.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494ZVK6|A0A494ZVK6_9BACI Bacteriocin aureocin A53 OS=Oceanobacillus halophilus OX=930130 GN=D8M06_16260 PE=4 SV=1
MM1 pKa = 7.26NRR3 pKa = 11.84TNPSINRR10 pKa = 11.84TNPSINRR17 pKa = 11.84TNPSINRR24 pKa = 11.84TNPSINRR31 pKa = 11.84TNPSINRR38 pKa = 11.84TNPSINRR45 pKa = 11.84TNPSINRR52 pKa = 11.84TNPSINRR59 pKa = 11.84TNPSINRR66 pKa = 11.84TSPSINRR73 pKa = 11.84TSPSINRR80 pKa = 11.84TNPSINHH87 pKa = 6.18TSPSINRR94 pKa = 11.84LQASTHH100 pKa = 5.41KK101 pKa = 10.8QPFAPFYY108 pKa = 10.25SKK110 pKa = 11.22QNIRR114 pKa = 3.69

Molecular weight: 12.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3727

0

3727

1103683

14

1747

296.1

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.563 ± 0.04

0.591 ± 0.011

5.325 ± 0.04

7.813 ± 0.055

4.525 ± 0.039

6.772 ± 0.037

2.012 ± 0.019

8.271 ± 0.048

6.894 ± 0.039

9.5 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.831 ± 0.02

4.813 ± 0.024

3.516 ± 0.024

3.625 ± 0.024

3.868 ± 0.03

6.02 ± 0.029

5.475 ± 0.026

6.97 ± 0.03

0.991 ± 0.014

3.624 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski