Clostridium sp. CAG:169

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1970 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6BSZ5|R6BSZ5_9CLOT HNH endonuclease domain protein OS=Clostridium sp. CAG:169 OX=1262778 GN=BN513_01937 PE=4 SV=1
MM1 pKa = 7.64TYY3 pKa = 11.23DD4 pKa = 4.44DD5 pKa = 5.22VITMLEE11 pKa = 4.05EE12 pKa = 4.46AGLPFAYY19 pKa = 10.25DD20 pKa = 3.46HH21 pKa = 6.26FAEE24 pKa = 5.39GEE26 pKa = 4.4SPDD29 pKa = 3.72PPFLVFLYY37 pKa = 9.99PGSDD41 pKa = 2.89NMFADD46 pKa = 3.44DD47 pKa = 4.24TVFKK51 pKa = 10.8KK52 pKa = 10.13IDD54 pKa = 3.47EE55 pKa = 4.6LNIEE59 pKa = 5.05LYY61 pKa = 10.57TDD63 pKa = 3.51VKK65 pKa = 11.09DD66 pKa = 4.63PEE68 pKa = 5.58AEE70 pKa = 4.17TQIEE74 pKa = 4.34DD75 pKa = 3.54TLIAHH80 pKa = 7.29DD81 pKa = 4.46LPYY84 pKa = 10.63EE85 pKa = 4.15KK86 pKa = 10.71SEE88 pKa = 3.77VWIEE92 pKa = 4.0SEE94 pKa = 3.82KK95 pKa = 10.87LYY97 pKa = 10.94EE98 pKa = 4.19VLYY101 pKa = 8.01QTQIIGGG108 pKa = 3.81

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6BC20|R6BC20_9CLOT ABC transmembrane type-1 domain-containing protein OS=Clostridium sp. CAG:169 OX=1262778 GN=BN513_00188 PE=3 SV=1
MM1 pKa = 7.32QAVCCLFVLVGGILPVAQFVHH22 pKa = 6.46PQLDD26 pKa = 3.78HH27 pKa = 7.25LSALHH32 pKa = 6.45HH33 pKa = 5.95RR34 pKa = 11.84RR35 pKa = 11.84CVKK38 pKa = 10.11ILAAHH43 pKa = 6.52GKK45 pKa = 7.64VCRR48 pKa = 11.84IKK50 pKa = 10.44LQRR53 pKa = 11.84VVYY56 pKa = 8.92LVPAQANGSDD66 pKa = 4.42DD67 pKa = 2.99IRR69 pKa = 11.84CRR71 pKa = 11.84VRR73 pKa = 11.84LRR75 pKa = 11.84EE76 pKa = 4.33HH77 pKa = 6.68ILDD80 pKa = 4.48FKK82 pKa = 11.41AGVDD86 pKa = 3.55VPLRR90 pKa = 11.84HH91 pKa = 6.58IMLLHH96 pKa = 6.79LSDD99 pKa = 4.06VLLGQQLVCFSLSHH113 pKa = 6.31NLHH116 pKa = 5.94NFKK119 pKa = 10.22GHH121 pKa = 6.35RR122 pKa = 11.84RR123 pKa = 11.84VQPVVNQIQHH133 pKa = 6.58DD134 pKa = 4.33AVTGTDD140 pKa = 3.18NLADD144 pKa = 3.59VAGAAFDD151 pKa = 3.7EE152 pKa = 4.71VLRR155 pKa = 11.84IAQPYY160 pKa = 8.51VGAVGQTGNLQQIRR174 pKa = 11.84KK175 pKa = 8.73ILRR178 pKa = 11.84LTIQQHH184 pKa = 5.93LPDD187 pKa = 4.1KK188 pKa = 10.69RR189 pKa = 11.84RR190 pKa = 11.84AHH192 pKa = 6.27LRR194 pKa = 11.84DD195 pKa = 3.21GKK197 pKa = 10.96GAGFAVDD204 pKa = 4.88LLRR207 pKa = 11.84CHH209 pKa = 5.83TQHH212 pKa = 6.86LRR214 pKa = 11.84RR215 pKa = 11.84GEE217 pKa = 4.14QAVHH221 pKa = 6.07LLVRR225 pKa = 11.84HH226 pKa = 5.74IRR228 pKa = 11.84IGDD231 pKa = 3.72LGVGQILHH239 pKa = 5.97VLVQGRR245 pKa = 11.84HH246 pKa = 4.93IVPEE250 pKa = 4.16DD251 pKa = 3.39VQLEE255 pKa = 4.32DD256 pKa = 4.71GVMQRR261 pKa = 11.84VKK263 pKa = 10.61IEE265 pKa = 3.77VGGDD269 pKa = 3.26DD270 pKa = 3.42VRR272 pKa = 11.84IDD274 pKa = 3.42VVRR277 pKa = 11.84RR278 pKa = 11.84MLDD281 pKa = 2.76RR282 pKa = 11.84GKK284 pKa = 9.93IEE286 pKa = 4.69HH287 pKa = 6.47IVIIRR292 pKa = 11.84HH293 pKa = 5.52NDD295 pKa = 2.59HH296 pKa = 7.0AARR299 pKa = 11.84VLTGGALDD307 pKa = 4.4PGAAFAQAVFLRR319 pKa = 11.84FMQGHH324 pKa = 5.92SPLVQVVFYY333 pKa = 10.36IAIGGLIGKK342 pKa = 7.93RR343 pKa = 11.84TGGPRR348 pKa = 11.84LKK350 pKa = 10.56DD351 pKa = 3.36VSLSEE356 pKa = 3.95QLLGVLVCLRR366 pKa = 11.84LVLTGKK372 pKa = 9.36VQVNIRR378 pKa = 11.84RR379 pKa = 11.84LVPVEE384 pKa = 3.62AQKK387 pKa = 10.96GFKK390 pKa = 10.13RR391 pKa = 11.84NVVTIPQIRR400 pKa = 11.84CATVFTVFRR409 pKa = 11.84RR410 pKa = 11.84QVKK413 pKa = 9.95ARR415 pKa = 11.84TDD417 pKa = 3.37RR418 pKa = 11.84AVGKK422 pKa = 9.36EE423 pKa = 3.94DD424 pKa = 3.5GVIAVWTAVVRR435 pKa = 11.84RR436 pKa = 11.84QRR438 pKa = 11.84IYY440 pKa = 11.11LRR442 pKa = 11.84DD443 pKa = 3.56ARR445 pKa = 11.84HH446 pKa = 6.57DD447 pKa = 3.79GDD449 pKa = 3.72KK450 pKa = 11.29GGTNRR455 pKa = 11.84TTRR458 pKa = 11.84AYY460 pKa = 10.54

Molecular weight: 51.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1970

0

1970

554686

30

1267

281.6

31.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.078 ± 0.055

1.829 ± 0.027

5.355 ± 0.046

6.936 ± 0.061

4.086 ± 0.04

7.195 ± 0.061

1.895 ± 0.026

6.304 ± 0.052

5.513 ± 0.049

9.981 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.991 ± 0.031

3.723 ± 0.038

3.791 ± 0.033

4.822 ± 0.054

4.942 ± 0.051

5.997 ± 0.05

5.141 ± 0.038

7.012 ± 0.051

0.895 ± 0.02

3.51 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski