Exophiala mesophila (Black yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D1ZBM4|A0A0D1ZBM4_EXOME Yippee domain-containing protein OS=Exophiala mesophila OX=212818 GN=PV10_06554 PE=3 SV=1
MM1 pKa = 7.61FSTLSISPSMLSISALTLTSLLVPSAAQTFTEE33 pKa = 4.86CNPLTQGGCPPDD45 pKa = 3.94PALGRR50 pKa = 11.84SVSVDD55 pKa = 3.85FTSGPSSEE63 pKa = 4.15FSAVGSTSYY72 pKa = 10.72TSDD75 pKa = 2.85GAAFTVARR83 pKa = 11.84SGDD86 pKa = 3.84SPSITSEE93 pKa = 3.56WYY95 pKa = 9.88IMFGHH100 pKa = 6.54VDD102 pKa = 4.14FVVKK106 pKa = 10.39AAPGTGIVSAAILQSDD122 pKa = 4.62CLDD125 pKa = 4.28EE126 pKa = 6.5IDD128 pKa = 4.05WEE130 pKa = 4.29WLGGDD135 pKa = 3.56NSQAQSNYY143 pKa = 8.23FGKK146 pKa = 11.16GITTTGYY153 pKa = 10.5NRR155 pKa = 11.84GAFHH159 pKa = 6.95GASNNHH165 pKa = 6.09DD166 pKa = 3.5QFHH169 pKa = 6.7TYY171 pKa = 10.38SIDD174 pKa = 2.94WTAEE178 pKa = 3.89QIVWSIDD185 pKa = 3.21GTTVRR190 pKa = 11.84AMSQAQAEE198 pKa = 4.46PGQYY202 pKa = 9.13PQTPMYY208 pKa = 11.08VKK210 pKa = 9.77MGAWAGGDD218 pKa = 3.55PANAPGTIEE227 pKa = 3.88WAGGYY232 pKa = 8.06TDD234 pKa = 4.61YY235 pKa = 11.44SQGPFTMYY243 pKa = 10.22VKK245 pKa = 10.46SVQVTDD251 pKa = 3.63YY252 pKa = 10.25STGSEE257 pKa = 4.08YY258 pKa = 10.36TYY260 pKa = 10.84SGSSGTWQSIQSNGGSINPEE280 pKa = 3.88GKK282 pKa = 8.6GTPVSPDD289 pKa = 2.93SAPAVTSVVPDD300 pKa = 3.41NGAPLGFGNNANRR313 pKa = 11.84EE314 pKa = 4.33SIAATRR320 pKa = 11.84TGYY323 pKa = 9.74PWTGTATASIATASTHH339 pKa = 4.52QTSDD343 pKa = 3.46SSWPSAPASDD353 pKa = 4.56SPSSPIVYY361 pKa = 9.02TSWDD365 pKa = 3.5DD366 pKa = 3.56QGFLTIVTTDD376 pKa = 3.62ASWLTAARR384 pKa = 11.84SYY386 pKa = 11.35DD387 pKa = 3.55EE388 pKa = 4.32QGFLITPSAPTIDD401 pKa = 3.4TGLPVPTTGPITTNSEE417 pKa = 3.79PDD419 pKa = 3.5PASTAGAVTAAPTTTFEE436 pKa = 4.1TAPNEE441 pKa = 4.09EE442 pKa = 4.23SQVPSNEE449 pKa = 4.28IITADD454 pKa = 3.45ATTTAIPSADD464 pKa = 3.35GTRR467 pKa = 11.84VAEE470 pKa = 4.35GDD472 pKa = 3.85SDD474 pKa = 4.42VSTADD479 pKa = 3.44STPVTSTTTGSEE491 pKa = 4.36SVPSSSSEE499 pKa = 4.07SSLSTATQTTGTDD512 pKa = 3.51AQTSATPSSDD522 pKa = 2.97SSNSPSATATSASEE536 pKa = 4.17VSATSTSLSSTKK548 pKa = 10.58EE549 pKa = 3.7SITRR553 pKa = 11.84SNSPTSTRR561 pKa = 11.84EE562 pKa = 3.62NTTLATSTEE571 pKa = 4.49SGASTTSASGTPPAPNGVGRR591 pKa = 11.84QGGMQPLGALLLGIVVLVLII611 pKa = 5.04

Molecular weight: 62.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1WS74|A0A0D1WS74_EXOME Transcription initiation factor IIE subunit alpha OS=Exophiala mesophila OX=212818 GN=PV10_06482 PE=3 SV=1
MM1 pKa = 6.79QVCDD5 pKa = 3.79RR6 pKa = 11.84VLGSWSNVVQGHH18 pKa = 5.37IRR20 pKa = 11.84LFRR23 pKa = 11.84RR24 pKa = 11.84GMSVMVYY31 pKa = 8.79QVFPSARR38 pKa = 11.84IMLALRR44 pKa = 11.84LAFDD48 pKa = 3.67MLKK51 pKa = 10.72AADD54 pKa = 4.35FPPAVRR60 pKa = 11.84GLSTLLSRR68 pKa = 11.84RR69 pKa = 11.84TAISIAKK76 pKa = 8.1EE77 pKa = 4.11LPHH80 pKa = 6.74SNPFVGSLTALASWWSGSALRR101 pKa = 11.84IFAILTPDD109 pKa = 3.59TLQVEE114 pKa = 4.95ACSMTAGLAPWGIVEE129 pKa = 4.97ALQILPRR136 pKa = 11.84TQASPRR142 pKa = 11.84YY143 pKa = 8.85EE144 pKa = 4.41SNIHH148 pKa = 6.37LNVILKK154 pKa = 10.23

Molecular weight: 16.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9181

819

10000

5129384

49

5074

512.9

56.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.371 ± 0.017

1.111 ± 0.008

5.888 ± 0.016

5.845 ± 0.02

3.659 ± 0.013

6.626 ± 0.024

2.517 ± 0.012

4.954 ± 0.015

4.769 ± 0.023

8.911 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.009

3.73 ± 0.011

6.192 ± 0.024

4.307 ± 0.015

6.097 ± 0.019

8.684 ± 0.028

6.191 ± 0.02

5.992 ± 0.016

1.383 ± 0.008

2.675 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski