Thalassospira sp. TSL5-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Thalassospiraceae; Thalassospira; unclassified Thalassospira

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3929 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4UUQ6|A0A1Q4UUQ6_9PROT Sulfurtransferase OS=Thalassospira sp. TSL5-1 OX=1544451 GN=LF95_04735 PE=4 SV=1
MM1 pKa = 7.74AYY3 pKa = 9.59IFTEE7 pKa = 4.41ADD9 pKa = 3.7DD10 pKa = 3.91PTQIAEE16 pKa = 4.39RR17 pKa = 11.84QWGQADD23 pKa = 4.29PIMFTTFTSCIGIMGIKK40 pKa = 9.48DD41 pKa = 3.43DD42 pKa = 3.94QVIGVHH48 pKa = 6.03LALMGTADD56 pKa = 4.16EE57 pKa = 5.13LVTNANIDD65 pKa = 3.47QAIALLDD72 pKa = 3.91GAANPVIIGQIEE84 pKa = 3.82IWEE87 pKa = 4.33DD88 pKa = 3.28AVPAVYY94 pKa = 9.89QHH96 pKa = 6.64LVSTLHH102 pKa = 6.36PVAIYY107 pKa = 9.85PRR109 pKa = 11.84DD110 pKa = 3.69DD111 pKa = 3.14GTYY114 pKa = 10.57GGRR117 pKa = 11.84NNQGNVQPLEE127 pKa = 4.17APP129 pKa = 3.67

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4UTN1|A0A1Q4UTN1_9PROT Antitoxin OS=Thalassospira sp. TSL5-1 OX=1544451 GN=LF95_02485 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.87GFRR19 pKa = 11.84SRR21 pKa = 11.84NATVGGRR28 pKa = 11.84RR29 pKa = 11.84VITARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3929

0

3929

1254152

41

4138

319.2

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.88 ± 0.05

0.96 ± 0.013

6.19 ± 0.037

5.553 ± 0.041

3.929 ± 0.025

8.181 ± 0.043

2.182 ± 0.02

6.083 ± 0.027

4.092 ± 0.031

9.797 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.022

3.407 ± 0.026

4.636 ± 0.029

3.438 ± 0.027

6.004 ± 0.033

5.589 ± 0.034

5.327 ± 0.035

7.217 ± 0.032

1.268 ± 0.016

2.467 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski