Amino acid dipepetide frequency for Nostocales cyanobacterium HT-58-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.385AlaAla: 7.385 ± 0.082	0.836AlaCys: 0.836 ± 0.022	3.82AlaAsp: 3.82 ± 0.044	4.919AlaGlu: 4.919 ± 0.061	3.107AlaPhe: 3.107 ± 0.049	5.419AlaGly: 5.419 ± 0.079	1.456AlaHis: 1.456 ± 0.032	6.36AlaIle: 6.36 ± 0.058	3.973AlaLys: 3.973 ± 0.057	8.795AlaLeu: 8.795 ± 0.091	1.667AlaMet: 1.667 ± 0.034	3.354AlaAsn: 3.354 ± 0.054	2.912AlaPro: 2.912 ± 0.053	4.431AlaGln: 4.431 ± 0.057	3.821AlaArg: 3.821 ± 0.05	4.997AlaSer: 4.997 ± 0.054	4.692AlaThr: 4.692 ± 0.059	5.868AlaVal: 5.868 ± 0.062	1.031AlaTrp: 1.031 ± 0.029	2.451AlaTyr: 2.451 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.703CysAla: 0.703 ± 0.018	0.184CysCys: 0.184 ± 0.011	0.557CysAsp: 0.557 ± 0.017	0.548CysGlu: 0.548 ± 0.016	0.424CysPhe: 0.424 ± 0.015	0.828CysGly: 0.828 ± 0.022	0.305CysHis: 0.305 ± 0.014	0.597CysIle: 0.597 ± 0.017	0.379CysLys: 0.379 ± 0.013	1.187CysLeu: 1.187 ± 0.032	0.169CysMet: 0.169 ± 0.008	0.372CysAsn: 0.372 ± 0.015	0.537CysPro: 0.537 ± 0.018	0.581CysGln: 0.581 ± 0.02	0.528CysArg: 0.528 ± 0.018	0.657CysSer: 0.657 ± 0.02	0.499CysThr: 0.499 ± 0.017	0.649CysVal: 0.649 ± 0.019	0.155CysTrp: 0.155 ± 0.01	0.374CysTyr: 0.374 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.649AspAla: 3.649 ± 0.05	0.478AspCys: 0.478 ± 0.017	2.056AspAsp: 2.056 ± 0.034	3.097AspGlu: 3.097 ± 0.05	2.211AspPhe: 2.211 ± 0.036	3.283AspGly: 3.283 ± 0.058	0.67AspHis: 0.67 ± 0.019	3.288AspIle: 3.288 ± 0.046	2.303AspLys: 2.303 ± 0.038	5.267AspLeu: 5.267 ± 0.058	0.766AspMet: 0.766 ± 0.021	1.931AspAsn: 1.931 ± 0.036	2.281AspPro: 2.281 ± 0.037	1.628AspGln: 1.628 ± 0.03	2.732AspArg: 2.732 ± 0.039	2.8AspSer: 2.8 ± 0.044	2.534AspThr: 2.534 ± 0.035	3.169AspVal: 3.169 ± 0.038	0.898AspTrp: 0.898 ± 0.023	1.731AspTyr: 1.731 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	5.477GluAla: 5.477 ± 0.062	0.532GluCys: 0.532 ± 0.021	2.705GluAsp: 2.705 ± 0.04	4.232GluGlu: 4.232 ± 0.055	2.458GluPhe: 2.458 ± 0.035	3.265GluGly: 3.265 ± 0.041	1.123GluHis: 1.123 ± 0.027	4.6GluIle: 4.6 ± 0.057	3.548GluLys: 3.548 ± 0.052	6.888GluLeu: 6.888 ± 0.078	1.283GluMet: 1.283 ± 0.025	2.654GluAsn: 2.654 ± 0.041	2.483GluPro: 2.483 ± 0.039	3.864GluGln: 3.864 ± 0.051	3.658GluArg: 3.658 ± 0.044	3.377GluSer: 3.377 ± 0.043	3.42GluThr: 3.42 ± 0.047	4.503GluVal: 4.503 ± 0.053	0.793GluTrp: 0.793 ± 0.019	1.889GluTyr: 1.889 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	3.207PheAla: 3.207 ± 0.041	0.553PheCys: 0.553 ± 0.017	2.165PheAsp: 2.165 ± 0.033	2.268PheGlu: 2.268 ± 0.032	1.791PhePhe: 1.791 ± 0.036	2.972PheGly: 2.972 ± 0.054	0.849PheHis: 0.849 ± 0.024	2.39PheIle: 2.39 ± 0.036	1.614PheLys: 1.614 ± 0.032	4.231PheLeu: 4.231 ± 0.051	0.687PheMet: 0.687 ± 0.019	1.622PheAsn: 1.622 ± 0.03	1.87PhePro: 1.87 ± 0.029	1.815PheGln: 1.815 ± 0.03	1.838PheArg: 1.838 ± 0.036	2.913PheSer: 2.913 ± 0.045	2.267PheThr: 2.267 ± 0.035	2.672PheVal: 2.672 ± 0.042	0.709PheTrp: 0.709 ± 0.02	1.352PheTyr: 1.352 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	4.921GlyAla: 4.921 ± 0.063	0.798GlyCys: 0.798 ± 0.022	3.171GlyAsp: 3.171 ± 0.046	4.124GlyGlu: 4.124 ± 0.044	2.99GlyPhe: 2.99 ± 0.044	4.809GlyGly: 4.809 ± 0.094	1.386GlyHis: 1.386 ± 0.036	4.955GlyIle: 4.955 ± 0.05	3.933GlyLys: 3.933 ± 0.044	6.854GlyLeu: 6.854 ± 0.072	1.468GlyMet: 1.468 ± 0.029	2.871GlyAsn: 2.871 ± 0.064	1.568GlyPro: 1.568 ± 0.036	3.005GlyGln: 3.005 ± 0.05	3.42GlyArg: 3.42 ± 0.046	4.173GlySer: 4.173 ± 0.068	3.869GlyThr: 3.869 ± 0.053	5.009GlyVal: 5.009 ± 0.062	1.117GlyTrp: 1.117 ± 0.027	2.363GlyTyr: 2.363 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	1.154HisAla: 1.154 ± 0.023	0.282HisCys: 0.282 ± 0.012	0.782HisAsp: 0.782 ± 0.02	1.028HisGlu: 1.028 ± 0.025	0.88HisPhe: 0.88 ± 0.023	1.182HisGly: 1.182 ± 0.024	0.63HisHis: 0.63 ± 0.022	1.252HisIle: 1.252 ± 0.027	0.881HisLys: 0.881 ± 0.025	2.366HisLeu: 2.366 ± 0.041	0.289HisMet: 0.289 ± 0.013	0.762HisAsn: 0.762 ± 0.018	1.364HisPro: 1.364 ± 0.027	1.203HisGln: 1.203 ± 0.031	1.123HisArg: 1.123 ± 0.028	1.395HisSer: 1.395 ± 0.027	1.039HisThr: 1.039 ± 0.022	0.918HisVal: 0.918 ± 0.022	0.382HisTrp: 0.382 ± 0.016	0.7HisTyr: 0.7 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.691IleAla: 6.691 ± 0.062	0.809IleCys: 0.809 ± 0.018	3.384IleAsp: 3.384 ± 0.038	4.081IleGlu: 4.081 ± 0.052	2.492IlePhe: 2.492 ± 0.038	4.485IleGly: 4.485 ± 0.049	1.329IleHis: 1.329 ± 0.025	3.494IleIle: 3.494 ± 0.049	2.878IleLys: 2.878 ± 0.042	6.578IleLeu: 6.578 ± 0.06	0.823IleMet: 0.823 ± 0.021	2.779IleAsn: 2.779 ± 0.046	3.485IlePro: 3.485 ± 0.043	2.948IleGln: 2.948 ± 0.039	3.126IleArg: 3.126 ± 0.044	4.349IleSer: 4.349 ± 0.049	3.586IleThr: 3.586 ± 0.047	4.359IleVal: 4.359 ± 0.052	0.876IleTrp: 0.876 ± 0.023	1.837IleTyr: 1.837 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.114LysAla: 4.114 ± 0.052	0.345LysCys: 0.345 ± 0.013	2.217LysAsp: 2.217 ± 0.035	2.959LysGlu: 2.959 ± 0.037	1.745LysPhe: 1.745 ± 0.029	2.749LysGly: 2.749 ± 0.048	0.905LysHis: 0.905 ± 0.022	3.282LysIle: 3.282 ± 0.043	2.468LysLys: 2.468 ± 0.038	5.372LysLeu: 5.372 ± 0.061	0.93LysMet: 0.93 ± 0.024	2.061LysAsn: 2.061 ± 0.037	2.479LysPro: 2.479 ± 0.043	2.954LysGln: 2.954 ± 0.047	2.53LysArg: 2.53 ± 0.038	3.002LysSer: 3.002 ± 0.043	3.004LysThr: 3.004 ± 0.047	3.426LysVal: 3.426 ± 0.047	0.508LysTrp: 0.508 ± 0.017	1.436LysTyr: 1.436 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	9.486LeuAla: 9.486 ± 0.076	1.11LeuCys: 1.11 ± 0.027	5.104LeuAsp: 5.104 ± 0.052	7.334LeuGlu: 7.334 ± 0.072	3.938LeuPhe: 3.938 ± 0.053	7.494LeuGly: 7.494 ± 0.065	1.991LeuHis: 1.991 ± 0.036	6.247LeuIle: 6.247 ± 0.065	5.524LeuLys: 5.524 ± 0.056	11.639LeuLeu: 11.639 ± 0.113	2.032LeuMet: 2.032 ± 0.032	4.6LeuAsn: 4.6 ± 0.051	5.764LeuPro: 5.764 ± 0.061	5.969LeuGln: 5.969 ± 0.063	5.755LeuArg: 5.755 ± 0.062	7.71LeuSer: 7.71 ± 0.077	6.475LeuThr: 6.475 ± 0.064	7.402LeuVal: 7.402 ± 0.072	1.487LeuTrp: 1.487 ± 0.036	2.7LeuTyr: 2.7 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.617MetAla: 1.617 ± 0.029	0.126MetCys: 0.126 ± 0.008	0.707MetAsp: 0.707 ± 0.022	0.982MetGlu: 0.982 ± 0.027	0.557MetPhe: 0.557 ± 0.018	1.29MetGly: 1.29 ± 0.027	0.36MetHis: 0.36 ± 0.014	1.056MetIle: 1.056 ± 0.025	0.943MetLys: 0.943 ± 0.024	1.967MetLeu: 1.967 ± 0.034	0.454MetMet: 0.454 ± 0.017	0.833MetAsn: 0.833 ± 0.02	0.935MetPro: 0.935 ± 0.022	0.95MetGln: 0.95 ± 0.02	0.979MetArg: 0.979 ± 0.024	1.35MetSer: 1.35 ± 0.027	1.396MetThr: 1.396 ± 0.026	1.292MetVal: 1.292 ± 0.026	0.181MetTrp: 0.181 ± 0.011	0.368MetTyr: 0.368 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.996AsnAla: 2.996 ± 0.048	0.472AsnCys: 0.472 ± 0.015	1.617AsnAsp: 1.617 ± 0.03	1.865AsnGlu: 1.865 ± 0.031	1.86AsnPhe: 1.86 ± 0.031	2.686AsnGly: 2.686 ± 0.052	0.866AsnHis: 0.866 ± 0.023	2.658AsnIle: 2.658 ± 0.049	1.728AsnLys: 1.728 ± 0.034	5.263AsnLeu: 5.263 ± 0.069	0.606AsnMet: 0.606 ± 0.019	1.89AsnAsn: 1.89 ± 0.04	2.779AsnPro: 2.779 ± 0.041	2.51AsnGln: 2.51 ± 0.047	2.325AsnArg: 2.325 ± 0.035	2.948AsnSer: 2.948 ± 0.052	2.274AsnThr: 2.274 ± 0.043	2.353AsnVal: 2.353 ± 0.044	0.7AsnTrp: 0.7 ± 0.019	1.395AsnTyr: 1.395 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	3.176ProAla: 3.176 ± 0.044	0.374ProCys: 0.374 ± 0.016	2.807ProAsp: 2.807 ± 0.044	3.823ProGlu: 3.823 ± 0.049	1.825ProPhe: 1.825 ± 0.032	3.112ProGly: 3.112 ± 0.045	0.947ProHis: 0.947 ± 0.022	3.04ProIle: 3.04 ± 0.044	2.306ProLys: 2.306 ± 0.04	4.764ProLeu: 4.764 ± 0.053	0.747ProMet: 0.747 ± 0.019	2.237ProAsn: 2.237 ± 0.039	2.267ProPro: 2.267 ± 0.044	2.64ProGln: 2.64 ± 0.043	1.848ProArg: 1.848 ± 0.031	3.116ProSer: 3.116 ± 0.047	2.965ProThr: 2.965 ± 0.046	3.496ProVal: 3.496 ± 0.042	0.602ProTrp: 0.602 ± 0.015	1.351ProTyr: 1.351 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.803GlnAla: 4.803 ± 0.051	0.351GlnCys: 0.351 ± 0.013	2.107GlnAsp: 2.107 ± 0.034	3.804GlnGlu: 3.804 ± 0.051	1.78GlnPhe: 1.78 ± 0.027	3.261GlnGly: 3.261 ± 0.045	0.931GlnHis: 0.931 ± 0.022	3.555GlnIle: 3.555 ± 0.044	2.98GlnLys: 2.98 ± 0.045	6.262GlnLeu: 6.262 ± 0.074	1.12GlnMet: 1.12 ± 0.024	2.075GlnAsn: 2.075 ± 0.037	2.666GlnPro: 2.666 ± 0.048	4.086GlnGln: 4.086 ± 0.071	3.118GlnArg: 3.118 ± 0.039	2.957GlnSer: 2.957 ± 0.042	3.066GlnThr: 3.066 ± 0.05	4.028GlnVal: 4.028 ± 0.053	0.669GlnTrp: 0.669 ± 0.018	1.275GlnTyr: 1.275 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	3.506ArgAla: 3.506 ± 0.046	0.549ArgCys: 0.549 ± 0.017	2.461ArgAsp: 2.461 ± 0.037	3.386ArgGlu: 3.386 ± 0.043	2.232ArgPhe: 2.232 ± 0.033	3.007ArgGly: 3.007 ± 0.042	1.063ArgHis: 1.063 ± 0.026	3.277ArgIle: 3.277 ± 0.044	2.442ArgLys: 2.442 ± 0.036	6.134ArgLeu: 6.134 ± 0.074	1.068ArgMet: 1.068 ± 0.022	2.136ArgAsn: 2.136 ± 0.032	2.142ArgPro: 2.142 ± 0.038	3.316ArgGln: 3.316 ± 0.045	3.199ArgArg: 3.199 ± 0.053	3.164ArgSer: 3.164 ± 0.038	2.664ArgThr: 2.664 ± 0.036	3.652ArgVal: 3.652 ± 0.05	0.805ArgTrp: 0.805 ± 0.02	1.93ArgTyr: 1.93 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	4.483SerAla: 4.483 ± 0.051	0.644SerCys: 0.644 ± 0.019	3.078SerAsp: 3.078 ± 0.043	3.67SerGlu: 3.67 ± 0.044	2.671SerPhe: 2.671 ± 0.038	4.787SerGly: 4.787 ± 0.065	1.45SerHis: 1.45 ± 0.028	3.753SerIle: 3.753 ± 0.039	2.808SerLys: 2.808 ± 0.037	7.287SerLeu: 7.287 ± 0.078	1.183SerMet: 1.183 ± 0.023	2.62SerAsn: 2.62 ± 0.045	3.437SerPro: 3.437 ± 0.047	3.736SerGln: 3.736 ± 0.042	3.28SerArg: 3.28 ± 0.045	4.759SerSer: 4.759 ± 0.066	3.766SerThr: 3.766 ± 0.053	4.431SerVal: 4.431 ± 0.056	0.907SerTrp: 0.907 ± 0.024	1.872SerTyr: 1.872 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.792ThrAla: 4.792 ± 0.063	0.474ThrCys: 0.474 ± 0.018	2.578ThrAsp: 2.578 ± 0.036	3.329ThrGlu: 3.329 ± 0.042	2.186ThrPhe: 2.186 ± 0.032	4.319ThrGly: 4.319 ± 0.054	1.162ThrHis: 1.162 ± 0.027	3.589ThrIle: 3.589 ± 0.046	2.368ThrLys: 2.368 ± 0.037	6.404ThrLeu: 6.404 ± 0.065	0.818ThrMet: 0.818 ± 0.023	2.253ThrAsn: 2.253 ± 0.035	3.493ThrPro: 3.493 ± 0.055	3.1ThrGln: 3.1 ± 0.048	2.56ThrArg: 2.56 ± 0.035	3.738ThrSer: 3.738 ± 0.055	3.52ThrThr: 3.52 ± 0.053	4.182ThrVal: 4.182 ± 0.055	0.698ThrTrp: 0.698 ± 0.021	1.675ThrTyr: 1.675 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	6.067ValAla: 6.067 ± 0.065	0.763ValCys: 0.763 ± 0.022	3.401ValAsp: 3.401 ± 0.044	4.671ValGlu: 4.671 ± 0.048	2.726ValPhe: 2.726 ± 0.041	4.843ValGly: 4.843 ± 0.054	1.156ValHis: 1.156 ± 0.022	4.444ValIle: 4.444 ± 0.051	3.44ValLys: 3.44 ± 0.043	7.208ValLeu: 7.208 ± 0.069	1.456ValMet: 1.456 ± 0.027	2.883ValAsn: 2.883 ± 0.041	3.15ValPro: 3.15 ± 0.044	3.166ValGln: 3.166 ± 0.046	3.511ValArg: 3.511 ± 0.05	4.488ValSer: 4.488 ± 0.051	3.998ValThr: 3.998 ± 0.047	5.211ValVal: 5.211 ± 0.049	0.905ValTrp: 0.905 ± 0.025	1.892ValTyr: 1.892 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	0.87TrpAla: 0.87 ± 0.024	0.164TrpCys: 0.164 ± 0.008	0.639TrpAsp: 0.639 ± 0.022	0.946TrpGlu: 0.946 ± 0.022	0.626TrpPhe: 0.626 ± 0.019	0.956TrpGly: 0.956 ± 0.025	0.367TrpHis: 0.367 ± 0.014	0.832TrpIle: 0.832 ± 0.024	0.641TrpLys: 0.641 ± 0.018	1.837TrpLeu: 1.837 ± 0.036	0.341TrpMet: 0.341 ± 0.014	0.649TrpAsn: 0.649 ± 0.02	0.214TrpPro: 0.214 ± 0.01	1.131TrpGln: 1.131 ± 0.028	0.877TrpArg: 0.877 ± 0.023	0.86TrpSer: 0.86 ± 0.024	0.635TrpThr: 0.635 ± 0.019	0.966TrpVal: 0.966 ± 0.023	0.247TrpTrp: 0.247 ± 0.012	0.428TrpTyr: 0.428 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.197TyrAla: 2.197 ± 0.034	0.388TyrCys: 0.388 ± 0.015	1.415TyrAsp: 1.415 ± 0.027	1.683TyrGlu: 1.683 ± 0.031	1.284TyrPhe: 1.284 ± 0.027	2.058TyrGly: 2.058 ± 0.039	0.668TyrHis: 0.668 ± 0.02	1.718TyrIle: 1.718 ± 0.032	1.33TyrLys: 1.33 ± 0.028	3.459TyrLeu: 3.459 ± 0.044	0.439TyrMet: 0.439 ± 0.016	1.208TyrAsn: 1.208 ± 0.025	1.618TyrPro: 1.618 ± 0.028	1.863TyrGln: 1.863 ± 0.032	1.906TyrArg: 1.906 ± 0.032	1.87TyrSer: 1.87 ± 0.03	1.568TyrThr: 1.568 ± 0.031	1.793TyrVal: 1.793 ± 0.032	0.554TyrTrp: 0.554 ± 0.016	1.029TyrTyr: 1.029 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6085 proteins (2015771 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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