Erwinia phage vB_EamM_Huxley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Machinavirus; unclassified Machinavirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2ICX9|A0A1B2ICX9_9CAUD Putative nuclease SbcCD D subunit OS=Erwinia phage vB_EamM_Huxley OX=1883373 GN=HUXLEY_44 PE=4 SV=1
MM1 pKa = 7.47TDD3 pKa = 3.59LYY5 pKa = 11.11EE6 pKa = 5.75DD7 pKa = 3.94YY8 pKa = 11.35ADD10 pKa = 3.55TLGLVRR16 pKa = 11.84QEE18 pKa = 3.8GRR20 pKa = 11.84LAPVLDD26 pKa = 4.19LVSLAIEE33 pKa = 4.12YY34 pKa = 10.25GYY36 pKa = 8.91DD37 pKa = 3.32TTLMDD42 pKa = 4.63IYY44 pKa = 10.35TGQIPDD50 pKa = 3.58MNEE53 pKa = 3.15EE54 pKa = 4.16CLRR57 pKa = 11.84LEE59 pKa = 4.4ALINRR64 pKa = 11.84CACALAQRR72 pKa = 11.84LGVGLNPQVAYY83 pKa = 10.17RR84 pKa = 11.84KK85 pKa = 8.09PKK87 pKa = 8.23EE88 pKa = 4.07VVRR91 pKa = 11.84ILHH94 pKa = 6.27GLTSAFEE101 pKa = 4.14EE102 pKa = 4.97FEE104 pKa = 4.44DD105 pKa = 3.93TDD107 pKa = 3.7TLYY110 pKa = 11.41GILLSGEE117 pKa = 3.88PPAYY121 pKa = 9.42ILEE124 pKa = 4.16NMVRR128 pKa = 11.84YY129 pKa = 10.09VYY131 pKa = 10.99GDD133 pKa = 3.41NDD135 pKa = 3.12IHH137 pKa = 8.28FEE139 pKa = 4.03DD140 pKa = 5.76LIVVVEE146 pKa = 3.97PRR148 pKa = 11.84VMNVMRR154 pKa = 11.84NFLAATSAEE163 pKa = 4.09QQAEE167 pKa = 4.41GTDD170 pKa = 3.4NPRR173 pKa = 11.84TQRR176 pKa = 11.84VVQYY180 pKa = 10.93LRR182 pKa = 11.84VYY184 pKa = 8.5PQNPSAYY191 pKa = 9.84VFLNLSDD198 pKa = 3.59TSDD201 pKa = 3.37INSVISALDD210 pKa = 3.61FSEE213 pKa = 4.24EE214 pKa = 4.24GGVGEE219 pKa = 5.27SEE221 pKa = 4.81LLTIYY226 pKa = 10.85AVGLSIIEE234 pKa = 4.04NDD236 pKa = 3.99TFDD239 pKa = 3.45EE240 pKa = 5.06AYY242 pKa = 10.31AALEE246 pKa = 4.19GMLEE250 pKa = 4.79LINSDD255 pKa = 4.26EE256 pKa = 4.28APEE259 pKa = 4.61DD260 pKa = 4.39PILRR264 pKa = 11.84DD265 pKa = 3.28GLAGLKK271 pKa = 10.08SIYY274 pKa = 10.5GEE276 pKa = 4.08EE277 pKa = 4.21EE278 pKa = 3.83QTDD281 pKa = 3.88EE282 pKa = 4.62PDD284 pKa = 2.91

Molecular weight: 31.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2ICX3|A0A1B2ICX3_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Huxley OX=1883373 GN=HUXLEY_45 PE=4 SV=1
MM1 pKa = 7.21LWEE4 pKa = 4.07IEE6 pKa = 4.48ANVEE10 pKa = 4.26TPVSSKK16 pKa = 10.51LRR18 pKa = 11.84ASHH21 pKa = 6.37LRR23 pKa = 11.84MRR25 pKa = 11.84RR26 pKa = 11.84LGAQPEE32 pKa = 4.19IPIGNGLLVRR42 pKa = 11.84IRR44 pKa = 11.84NIYY47 pKa = 8.84VDD49 pKa = 4.11GYY51 pKa = 10.7FYY53 pKa = 11.31SKK55 pKa = 11.0NKK57 pKa = 8.91TVGALSTVTPAKK69 pKa = 10.56VSTLRR74 pKa = 11.84VGRR77 pKa = 11.84KK78 pKa = 9.1PDD80 pKa = 3.35VPPNAGLLANTVKK93 pKa = 10.67AYY95 pKa = 8.88PCRR98 pKa = 3.91

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

271

0

271

76086

35

2463

280.8

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.987 ± 0.166

0.919 ± 0.058

6.377 ± 0.107

6.179 ± 0.171

4.034 ± 0.086

6.564 ± 0.203

2.037 ± 0.085

5.482 ± 0.102

5.378 ± 0.13

8.978 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.062

5.007 ± 0.09

4.462 ± 0.099

3.533 ± 0.086

5.552 ± 0.117

5.788 ± 0.112

6.464 ± 0.132

7.124 ± 0.116

1.389 ± 0.049

3.923 ± 0.085
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski