Lachnotalea sp. AF33-28

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnotalea; unclassified Lachnotalea

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A416ESW3|A0A416ESW3_9FIRM Extracellular solute-binding protein OS=Lachnotalea sp. AF33-28 OX=2292046 GN=DWZ56_01175 PE=4 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84RR3 pKa = 11.84CAISRR8 pKa = 11.84IAEE11 pKa = 3.85FAVYY15 pKa = 9.78EE16 pKa = 4.04FTMDD20 pKa = 4.03GLCIVMLINTLSCMKK35 pKa = 10.4GCFVVSGQTFGGKK48 pKa = 8.29NMKK51 pKa = 10.0KK52 pKa = 9.99LLAIILTCALAVSLAACSAPAPKK75 pKa = 9.25EE76 pKa = 4.2TDD78 pKa = 3.1VSTAAAEE85 pKa = 4.41TTQTTKK91 pKa = 10.83AAEE94 pKa = 4.37TPEE97 pKa = 4.4TTQSAGTEE105 pKa = 4.07QTAEE109 pKa = 3.94TSAAALPTTDD119 pKa = 4.39PSGAQIKK126 pKa = 9.95VPEE129 pKa = 5.15DD130 pKa = 3.21IEE132 pKa = 4.61TIAVLSPSIAQTIIGLGLGDD152 pKa = 5.39KK153 pKa = 10.2IAAYY157 pKa = 9.69DD158 pKa = 3.73AQSVGLEE165 pKa = 4.14GLPEE169 pKa = 4.25SDD171 pKa = 3.22IVLDD175 pKa = 4.55FMQPDD180 pKa = 4.06MEE182 pKa = 4.74QLAALNPDD190 pKa = 3.51VLFVSNMTLYY200 pKa = 10.73DD201 pKa = 3.53QEE203 pKa = 5.01NPYY206 pKa = 8.35QTLIDD211 pKa = 3.44QGVCVICVPTSEE223 pKa = 4.97SIAAIQSDD231 pKa = 3.84ISFLAAVLGVPEE243 pKa = 4.89KK244 pKa = 11.06GEE246 pKa = 4.0AMLADD251 pKa = 3.95MQAQIDD257 pKa = 4.26SIAAIGSTITDD268 pKa = 3.03KK269 pKa = 10.54KK270 pKa = 8.93TVYY273 pKa = 10.49FEE275 pKa = 3.77IAAAPSMYY283 pKa = 10.53SFGSGVFLNEE293 pKa = 3.88MIEE296 pKa = 4.6LIGAKK301 pKa = 10.22NILADD306 pKa = 3.58QNGWLGVEE314 pKa = 4.24AEE316 pKa = 4.56TVVAANPDD324 pKa = 3.8VILTNVNYY332 pKa = 10.03IEE334 pKa = 5.21NPTQEE339 pKa = 3.91IMDD342 pKa = 3.94RR343 pKa = 11.84EE344 pKa = 4.54GWADD348 pKa = 3.33MTAVADD354 pKa = 3.77KK355 pKa = 10.76SVYY358 pKa = 10.85YY359 pKa = 9.34IDD361 pKa = 4.95NMASSSSNQNIVKK374 pKa = 10.38ALVQMAEE381 pKa = 4.13AIYY384 pKa = 9.99PEE386 pKa = 5.06YY387 pKa = 9.76YY388 pKa = 10.11TEE390 pKa = 4.0

Molecular weight: 41.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A416EL82|A0A416EL82_9FIRM LysR family transcriptional regulator OS=Lachnotalea sp. AF33-28 OX=2292046 GN=DWZ56_08475 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.43TRR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.02VHH16 pKa = 5.68GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4332

0

4332

1520675

26

3006

351.0

39.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.888 ± 0.04

1.671 ± 0.014

5.601 ± 0.027

7.285 ± 0.039

4.197 ± 0.024

7.33 ± 0.037

1.79 ± 0.016

6.684 ± 0.031

5.665 ± 0.027

9.579 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.128 ± 0.019

3.911 ± 0.022

3.64 ± 0.019

3.246 ± 0.02

5.218 ± 0.029

5.874 ± 0.028

5.178 ± 0.026

6.587 ± 0.029

1.152 ± 0.016

4.376 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski