Escherichia phage vB_EcoS_XY1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XM44|A0A6G6XM44_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_XY1 OX=2681674 GN=GT371_00062 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.45VKK4 pKa = 10.07AAKK7 pKa = 9.99ILWLAALDD15 pKa = 3.94YY16 pKa = 9.28VTEE19 pKa = 4.24GKK21 pKa = 10.81VYY23 pKa = 10.77DD24 pKa = 4.4VYY26 pKa = 11.72NPTATGFGYY35 pKa = 10.02IDD37 pKa = 4.76SDD39 pKa = 3.9DD40 pKa = 3.69DD41 pKa = 4.32CYY43 pKa = 11.52IFIDD47 pKa = 4.21FGNDD51 pKa = 2.84EE52 pKa = 4.37GDD54 pKa = 3.54GFHH57 pKa = 6.01GVKK60 pKa = 9.66WEE62 pKa = 3.94IVEE65 pKa = 4.09

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XM48|A0A6G6XM48_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_XY1 OX=2681674 GN=GT371_00069 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.57LNDD5 pKa = 3.12IVTWTGRR12 pKa = 11.84NGEE15 pKa = 4.23KK16 pKa = 10.46RR17 pKa = 11.84FGLIVASDD25 pKa = 3.7GNKK28 pKa = 8.34YY29 pKa = 10.45RR30 pKa = 11.84VMRR33 pKa = 11.84WCQWARR39 pKa = 11.84RR40 pKa = 11.84PRR42 pKa = 11.84YY43 pKa = 7.96YY44 pKa = 10.15WIRR47 pKa = 11.84GEE49 pKa = 4.05RR50 pKa = 11.84LSLKK54 pKa = 10.19HH55 pKa = 5.63QQ56 pKa = 3.68

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13914

33

890

183.1

20.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.681 ± 0.529

1.078 ± 0.126

6.188 ± 0.272

6.763 ± 0.443

3.924 ± 0.154

7.611 ± 0.245

1.718 ± 0.198

5.347 ± 0.187

5.814 ± 0.424

7.316 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.134

4.269 ± 0.259

3.802 ± 0.242

3.5 ± 0.303

5.448 ± 0.24

5.929 ± 0.384

6.281 ± 0.385

7.345 ± 0.259

1.531 ± 0.166

3.816 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski