Clostridium sp. CAG:417

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1291 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6SLF2|R6SLF2_9CLOT Toxin-antitoxin system antitoxin component Xre family OS=Clostridium sp. CAG:417 OX=1262804 GN=BN650_01012 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.34LEE4 pKa = 4.44KK5 pKa = 9.9YY6 pKa = 10.44LPIGSVVLLDD16 pKa = 3.91GGEE19 pKa = 4.23TKK21 pKa = 10.99VMITGFVASAEE32 pKa = 4.44DD33 pKa = 3.61EE34 pKa = 4.54NEE36 pKa = 3.87VYY38 pKa = 10.47DD39 pKa = 4.73YY40 pKa = 10.77IGCIYY45 pKa = 10.13PEE47 pKa = 4.51GVTDD51 pKa = 3.7TEE53 pKa = 4.19EE54 pKa = 4.01NLLFNHH60 pKa = 6.23DD61 pKa = 4.37QIVQIYY67 pKa = 9.41HH68 pKa = 7.01LGLIDD73 pKa = 5.34DD74 pKa = 5.26DD75 pKa = 4.62EE76 pKa = 6.58IIYY79 pKa = 10.79KK80 pKa = 10.21NALEE84 pKa = 4.03EE85 pKa = 4.18TMNEE89 pKa = 3.63IDD91 pKa = 4.19NEE93 pKa = 4.07NDD95 pKa = 3.31NEE97 pKa = 4.21VEE99 pKa = 5.01NIIEE103 pKa = 4.15PNISQPEE110 pKa = 4.13MTQGGATEE118 pKa = 4.37PVTIKK123 pKa = 10.65PVMEE127 pKa = 4.46NFNTGNSFFEE137 pKa = 4.7DD138 pKa = 3.24

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6TU43|R6TU43_9CLOT DNA polymerase IV 1 OS=Clostridium sp. CAG:417 OX=1262804 GN=BN650_01079 PE=3 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.36KK4 pKa = 10.89SMIVKK9 pKa = 10.42ANRR12 pKa = 11.84KK13 pKa = 7.69PKK15 pKa = 9.85YY16 pKa = 9.64KK17 pKa = 9.6VRR19 pKa = 11.84EE20 pKa = 3.83YY21 pKa = 10.14TRR23 pKa = 11.84CSRR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 7.73AVMSKK36 pKa = 10.27FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.62KK58 pKa = 10.92SSWW61 pKa = 3.1

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1291

0

1291

381582

30

1801

295.6

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.388 ± 0.056

1.156 ± 0.026

6.105 ± 0.054

6.989 ± 0.074

4.294 ± 0.051

5.162 ± 0.062

1.379 ± 0.025

9.692 ± 0.08

9.982 ± 0.075

9.205 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.033

7.483 ± 0.084

2.462 ± 0.033

2.41 ± 0.039

3.206 ± 0.045

6.522 ± 0.059

5.499 ± 0.052

6.082 ± 0.059

0.514 ± 0.014

5.092 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski