Impatiens necrotic spot virus (INSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Impatiens necrotic spot orthotospovirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|Q01268|MVP_INSV Movement protein OS=Impatiens necrotic spot virus OX=11612 PE=3 SV=1
MM1 pKa = 7.5ALKK4 pKa = 9.09EE5 pKa = 4.13TDD7 pKa = 3.12AKK9 pKa = 10.65IHH11 pKa = 5.38VEE13 pKa = 4.04RR14 pKa = 11.84GDD16 pKa = 3.39HH17 pKa = 6.65PEE19 pKa = 4.62IYY21 pKa = 10.51DD22 pKa = 3.32EE23 pKa = 4.74AYY25 pKa = 9.53YY26 pKa = 10.81DD27 pKa = 4.18RR28 pKa = 11.84SVDD31 pKa = 3.56HH32 pKa = 6.96KK33 pKa = 11.71NEE35 pKa = 3.91ILDD38 pKa = 3.82TLAEE42 pKa = 4.09MLQNATGKK50 pKa = 7.06TLRR53 pKa = 11.84PTRR56 pKa = 11.84DD57 pKa = 3.33TQTVLANNEE66 pKa = 4.16VPQSSSGLSSTPTTISIMDD85 pKa = 4.59LPNPCLNASSLTCSIKK101 pKa = 10.62GVSTFNVYY109 pKa = 10.22YY110 pKa = 9.94QVEE113 pKa = 4.75SNGVIYY119 pKa = 10.66SCISDD124 pKa = 4.49TITKK128 pKa = 10.21LGNCEE133 pKa = 4.02GSSEE137 pKa = 4.11LPRR140 pKa = 11.84SFEE143 pKa = 4.21TVPVVPITKK152 pKa = 9.22IDD154 pKa = 3.73NKK156 pKa = 10.33RR157 pKa = 11.84KK158 pKa = 10.08LSIGTKK164 pKa = 9.78FYY166 pKa = 10.47IIEE169 pKa = 4.07SLEE172 pKa = 4.07NYY174 pKa = 9.35NYY176 pKa = 10.33PIMYY180 pKa = 10.06NSRR183 pKa = 11.84PTNGTVSLQSVKK195 pKa = 10.37FSGDD199 pKa = 3.39CKK201 pKa = 10.19ISKK204 pKa = 9.05TNIVNSYY211 pKa = 8.38TVSLTTPEE219 pKa = 4.65KK220 pKa = 10.36IMGYY224 pKa = 6.84VVKK227 pKa = 10.74RR228 pKa = 11.84EE229 pKa = 3.98GSDD232 pKa = 3.15MSHH235 pKa = 7.26SIISFSGSVSLTFTEE250 pKa = 4.27EE251 pKa = 4.06NMDD254 pKa = 3.58GKK256 pKa = 10.31HH257 pKa = 6.47NLLCGDD263 pKa = 4.15KK264 pKa = 10.33SSKK267 pKa = 10.44VPLVDD272 pKa = 4.11KK273 pKa = 10.66RR274 pKa = 11.84VRR276 pKa = 11.84DD277 pKa = 4.21CIIKK281 pKa = 9.92YY282 pKa = 10.26SKK284 pKa = 10.79NIYY287 pKa = 9.4KK288 pKa = 8.29QTACINFSWFRR299 pKa = 11.84LIMIALIVYY308 pKa = 7.54FPIRR312 pKa = 11.84YY313 pKa = 8.5LVNKK317 pKa = 8.41TSKK320 pKa = 9.24TLFYY324 pKa = 10.53GYY326 pKa = 10.61DD327 pKa = 3.51LLGLITYY334 pKa = 7.77PILLLINYY342 pKa = 7.32LWSYY346 pKa = 10.77FPLKK350 pKa = 10.76CKK352 pKa = 10.73VCGNLCLVTHH362 pKa = 6.93EE363 pKa = 4.52CSKK366 pKa = 11.31LCICNKK372 pKa = 9.76NKK374 pKa = 10.29ASEE377 pKa = 4.16EE378 pKa = 3.99HH379 pKa = 7.07SEE381 pKa = 4.05EE382 pKa = 4.33CPIITRR388 pKa = 11.84TAEE391 pKa = 3.92KK392 pKa = 10.15NKK394 pKa = 9.75KK395 pKa = 8.79YY396 pKa = 10.53NWASIEE402 pKa = 4.06WFHH405 pKa = 7.61LIVNTKK411 pKa = 10.01IGLSFLKK418 pKa = 10.62AVTEE422 pKa = 4.06TLIGFLILSQMPMSMAQTAQCLDD445 pKa = 2.97SCYY448 pKa = 10.46YY449 pKa = 10.91VPGCDD454 pKa = 2.32RR455 pKa = 11.84FVTNRR460 pKa = 11.84YY461 pKa = 8.59DD462 pKa = 3.21KK463 pKa = 11.24CPEE466 pKa = 3.91KK467 pKa = 10.75DD468 pKa = 3.21QCFCAIKK475 pKa = 10.07EE476 pKa = 4.05NSIVEE481 pKa = 4.63SNFLTNVVTEE491 pKa = 4.61GPMDD495 pKa = 5.18CIPYY499 pKa = 9.07QEE501 pKa = 4.41CKK503 pKa = 10.82GRR505 pKa = 11.84ITEE508 pKa = 4.09NALVTFVKK516 pKa = 10.4CRR518 pKa = 11.84FGCEE522 pKa = 3.71YY523 pKa = 11.3ASIFQSKK530 pKa = 9.64PLDD533 pKa = 3.75NGFLEE538 pKa = 4.42YY539 pKa = 10.95SGDD542 pKa = 3.86TLGLNAVNLHH552 pKa = 4.84FMKK555 pKa = 10.75RR556 pKa = 11.84LRR558 pKa = 11.84NGIIDD563 pKa = 4.45FYY565 pKa = 11.59NKK567 pKa = 8.26TEE569 pKa = 3.88KK570 pKa = 10.57YY571 pKa = 10.71GYY573 pKa = 10.07ISGDD577 pKa = 3.16ALKK580 pKa = 10.85SNEE583 pKa = 3.86SDD585 pKa = 2.97IPEE588 pKa = 4.59SIFPRR593 pKa = 11.84KK594 pKa = 9.73SLIFDD599 pKa = 3.5SVIDD603 pKa = 3.76GKK605 pKa = 10.34YY606 pKa = 9.67RR607 pKa = 11.84YY608 pKa = 9.11MIEE611 pKa = 4.0EE612 pKa = 4.28SLLSGGGTVFSLNDD626 pKa = 3.62KK627 pKa = 10.76SSSTAQKK634 pKa = 9.78FVVYY638 pKa = 9.74IKK640 pKa = 10.38KK641 pKa = 9.93VRR643 pKa = 11.84IQYY646 pKa = 9.9DD647 pKa = 3.22VSEE650 pKa = 4.73QYY652 pKa = 7.67TTAPIQSTHH661 pKa = 5.61TDD663 pKa = 3.8FFSTCTGKK671 pKa = 10.88CSDD674 pKa = 4.38CRR676 pKa = 11.84KK677 pKa = 8.46EE678 pKa = 4.01QPITGYY684 pKa = 10.63QDD686 pKa = 3.33FCITPTSYY694 pKa = 9.62WGCEE698 pKa = 4.11EE699 pKa = 4.3VWCLAINEE707 pKa = 4.4GATCGFCRR715 pKa = 11.84NVYY718 pKa = 11.13DD719 pKa = 3.45MDD721 pKa = 3.6QSFRR725 pKa = 11.84IYY727 pKa = 10.86SVIKK731 pKa = 9.22STIKK735 pKa = 10.72SEE737 pKa = 4.06VCISGFVGAKK747 pKa = 9.44CFTVSEE753 pKa = 4.44EE754 pKa = 4.22VPSEE758 pKa = 4.0SGYY761 pKa = 10.39FQADD765 pKa = 3.04ILADD769 pKa = 3.62FHH771 pKa = 8.0NDD773 pKa = 2.74GLTIGQLIAHH783 pKa = 7.06GPDD786 pKa = 2.82SHH788 pKa = 8.61VYY790 pKa = 9.58AGNIARR796 pKa = 11.84LNNPSKK802 pKa = 10.61MFGHH806 pKa = 7.02PQLSHH811 pKa = 6.7QGDD814 pKa = 4.2PIFSKK819 pKa = 9.6KK820 pKa = 8.86TLDD823 pKa = 3.75TNDD826 pKa = 4.41LSWDD830 pKa = 3.46CSAIGKK836 pKa = 8.21KK837 pKa = 9.01TITIKK842 pKa = 10.49SCGYY846 pKa = 8.39DD847 pKa = 2.98TYY849 pKa = 11.34RR850 pKa = 11.84FKK852 pKa = 10.79TGLNQISDD860 pKa = 3.76IPVQFTDD867 pKa = 3.21QNSFYY872 pKa = 9.67MEE874 pKa = 4.96KK875 pKa = 10.06IFSLGKK881 pKa = 10.4LKK883 pKa = 10.36IVLDD887 pKa = 4.15LPSEE891 pKa = 4.15LFKK894 pKa = 10.56TVPKK898 pKa = 10.58KK899 pKa = 10.55PILSSVSLSCKK910 pKa = 10.43GCFLCSQGLRR920 pKa = 11.84CAASFISDD928 pKa = 3.16ITFSARR934 pKa = 11.84LTMKK938 pKa = 9.95QCSLSTYY945 pKa = 10.14QIAVKK950 pKa = 10.49KK951 pKa = 10.2GANKK955 pKa = 10.31YY956 pKa = 9.72NLTMFCTSNPEE967 pKa = 3.89KK968 pKa = 10.32QKK970 pKa = 10.68MIIEE974 pKa = 4.3PEE976 pKa = 3.94GDD978 pKa = 2.84KK979 pKa = 10.99SYY981 pKa = 11.38SVEE984 pKa = 3.92ALVDD988 pKa = 3.94SVAVLEE994 pKa = 4.54PEE996 pKa = 4.99NIIDD1000 pKa = 4.54QNDD1003 pKa = 3.33QHH1005 pKa = 7.97AHH1007 pKa = 6.35EE1008 pKa = 4.39EE1009 pKa = 3.97QQYY1012 pKa = 11.41NSDD1015 pKa = 3.46TSVWSFWDD1023 pKa = 3.81YY1024 pKa = 11.48VKK1026 pKa = 11.19SPFNFIASHH1035 pKa = 6.48FGSFFDD1041 pKa = 3.63TVRR1044 pKa = 11.84VVLLILFVFALAYY1057 pKa = 10.1LCSIVATMCRR1067 pKa = 11.84GYY1069 pKa = 10.84VRR1071 pKa = 11.84NKK1073 pKa = 10.21SYY1075 pKa = 8.32KK1076 pKa = 8.2TKK1078 pKa = 10.78YY1079 pKa = 10.15IEE1081 pKa = 4.29DD1082 pKa = 3.9TNDD1085 pKa = 2.96YY1086 pKa = 10.38SLVSTSSGKK1095 pKa = 8.74DD1096 pKa = 2.89TITRR1100 pKa = 11.84RR1101 pKa = 11.84RR1102 pKa = 11.84PPLDD1106 pKa = 3.3FSGII1110 pKa = 3.76

Molecular weight:
124.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|Q01268|MVP_INSV Movement protein OS=Impatiens necrotic spot virus OX=11612 PE=3 SV=1
MM1 pKa = 7.48NSFFKK6 pKa = 10.63SLRR9 pKa = 11.84SSSSRR14 pKa = 11.84EE15 pKa = 3.47LDD17 pKa = 3.28HH18 pKa = 7.32PRR20 pKa = 11.84VTTTLSKK27 pKa = 10.5QGADD31 pKa = 2.45IVVHH35 pKa = 6.1NPSANHH41 pKa = 5.76NNKK44 pKa = 9.51EE45 pKa = 4.1VLQRR49 pKa = 11.84AMDD52 pKa = 3.77SSKK55 pKa = 11.32GKK57 pKa = 10.18ILMNNTGTSSLGTYY71 pKa = 10.02EE72 pKa = 4.11SDD74 pKa = 3.4QISEE78 pKa = 4.31SEE80 pKa = 4.44SYY82 pKa = 10.89DD83 pKa = 3.18LSARR87 pKa = 11.84MIVDD91 pKa = 3.93TNHH94 pKa = 7.28HH95 pKa = 5.74ISSWKK100 pKa = 9.82NDD102 pKa = 3.42LFVGNGDD109 pKa = 3.39KK110 pKa = 10.99AATKK114 pKa = 9.89IIKK117 pKa = 9.32IHH119 pKa = 5.57PTWDD123 pKa = 3.06SRR125 pKa = 11.84KK126 pKa = 8.54QYY128 pKa = 10.8MMISRR133 pKa = 11.84IVIWICPTIADD144 pKa = 4.47PDD146 pKa = 3.85GKK148 pKa = 10.69LAVALIDD155 pKa = 3.91PNKK158 pKa = 10.21SVNARR163 pKa = 11.84TVLKK167 pKa = 10.4GQGSIKK173 pKa = 10.74DD174 pKa = 3.77PICFVFYY181 pKa = 10.61LNWSIPKK188 pKa = 9.92VNNTSEE194 pKa = 4.29NCVQLHH200 pKa = 6.15LLCDD204 pKa = 3.46QVYY207 pKa = 10.33KK208 pKa = 10.68KK209 pKa = 10.4DD210 pKa = 3.56VSFASVMYY218 pKa = 9.84SWTKK222 pKa = 9.77EE223 pKa = 3.95FCDD226 pKa = 4.72SPRR229 pKa = 11.84ADD231 pKa = 4.0LDD233 pKa = 3.82KK234 pKa = 11.18SCMIIPINRR243 pKa = 11.84AIRR246 pKa = 11.84AKK248 pKa = 9.89SQAFIEE254 pKa = 4.45ACKK257 pKa = 10.62LIIPKK262 pKa = 10.35GNSEE266 pKa = 3.96KK267 pKa = 10.61QIRR270 pKa = 11.84RR271 pKa = 11.84QLAEE275 pKa = 3.85LSANLEE281 pKa = 3.98KK282 pKa = 10.85SVEE285 pKa = 4.1EE286 pKa = 4.31EE287 pKa = 4.85EE288 pKa = 4.85NVTDD292 pKa = 3.94NKK294 pKa = 10.67IEE296 pKa = 3.8ISFDD300 pKa = 3.43NEE302 pKa = 3.48II303 pKa = 4.09

Molecular weight:
34.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1413

303

1110

706.5

79.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.317 ± 0.808

3.68 ± 0.682

5.662 ± 0.468

5.308 ± 0.151

4.6 ± 0.812

4.954 ± 0.823

1.769 ± 0.269

8.209 ± 0.514

7.502 ± 0.373

7.431 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.052 ± 0.293

5.662 ± 0.796

3.822 ± 0.095

3.114 ± 0.093

3.397 ± 0.445

10.616 ± 0.466

6.582 ± 0.977

6.016 ± 0.037

0.991 ± 0.328

4.317 ± 1.164

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski