Red clover powdery mildew-associated totivirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S3Q2X1|A0A0S3Q2X1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 2 OX=1714363 GN=RdRp PE=3 SV=1
MM1 pKa = 8.05DD2 pKa = 4.69SMVFDD7 pKa = 3.69TCVDD11 pKa = 3.43NVTSLLYY18 pKa = 10.58NLLRR22 pKa = 11.84MYY24 pKa = 10.74YY25 pKa = 9.71ILEE28 pKa = 4.26NKK30 pKa = 9.17NLSVKK35 pKa = 9.49KK36 pKa = 9.0TDD38 pKa = 2.91SYY40 pKa = 11.77YY41 pKa = 10.92DD42 pKa = 4.14DD43 pKa = 3.82GHH45 pKa = 8.39LSVDD49 pKa = 3.17MGDD52 pKa = 3.22VFGVGEE58 pKa = 4.03RR59 pKa = 11.84SKK61 pKa = 10.97RR62 pKa = 11.84ITDD65 pKa = 3.28VRR67 pKa = 11.84FNPCRR72 pKa = 11.84AHH74 pKa = 7.34RR75 pKa = 11.84YY76 pKa = 8.76DD77 pKa = 3.84AEE79 pKa = 4.64TYY81 pKa = 9.53FLNFSGEE88 pKa = 4.43PVLHH92 pKa = 6.15MANANALSSGILATALGEE110 pKa = 4.28WSSEE114 pKa = 3.75TPYY117 pKa = 10.75AISHH121 pKa = 7.01PSPALAQNIIINTRR135 pKa = 11.84GGVSQDD141 pKa = 3.46EE142 pKa = 4.3IEE144 pKa = 4.58SFNQITTNDD153 pKa = 3.44VLKK156 pKa = 10.54VIKK159 pKa = 10.44DD160 pKa = 3.31IVEE163 pKa = 4.19SNRR166 pKa = 11.84YY167 pKa = 8.02HH168 pKa = 6.47AQFEE172 pKa = 4.34LAYY175 pKa = 11.22LMLAQLLFTLKK186 pKa = 10.12PRR188 pKa = 11.84SAEE191 pKa = 3.74ALIWMHH197 pKa = 6.01GTPLMQLPKK206 pKa = 10.73FMTFRR211 pKa = 11.84GLVNEE216 pKa = 4.49TVSLAPYY223 pKa = 9.95VPFPEE228 pKa = 5.26RR229 pKa = 11.84LDD231 pKa = 3.82TYY233 pKa = 8.94GTWLANKK240 pKa = 9.0EE241 pKa = 3.99AIYY244 pKa = 10.4YY245 pKa = 10.22HH246 pKa = 6.49SLLINEE252 pKa = 4.52VVMTYY257 pKa = 10.3AYY259 pKa = 9.97EE260 pKa = 5.13AITTNDD266 pKa = 2.65ITRR269 pKa = 11.84LLLRR273 pKa = 11.84QIGGIFSAEE282 pKa = 4.07PGLMADD288 pKa = 4.29MSCFAQVLQKK298 pKa = 10.75EE299 pKa = 4.44VLLPFEE305 pKa = 4.69TNGGLDD311 pKa = 3.0RR312 pKa = 11.84FTWINCLDD320 pKa = 4.05GEE322 pKa = 4.35IGMQVKK328 pKa = 10.41VNDD331 pKa = 4.23PNAQHH336 pKa = 7.22HH337 pKa = 6.09YY338 pKa = 10.68RR339 pKa = 11.84LDD341 pKa = 3.65TLEE344 pKa = 5.56LDD346 pKa = 4.32DD347 pKa = 4.65GVIRR351 pKa = 11.84HH352 pKa = 5.79TLRR355 pKa = 11.84LDD357 pKa = 3.29SFVPYY362 pKa = 10.09VYY364 pKa = 10.15PVLLYY369 pKa = 10.48GYY371 pKa = 10.07APSTYY376 pKa = 9.32YY377 pKa = 10.96QNDD380 pKa = 3.69CVRR383 pKa = 11.84EE384 pKa = 3.96VEE386 pKa = 4.29MKK388 pKa = 10.05IDD390 pKa = 3.7SKK392 pKa = 10.48KK393 pKa = 10.55EE394 pKa = 3.7RR395 pKa = 11.84MITEE399 pKa = 4.21SVDD402 pKa = 3.86DD403 pKa = 4.2FSKK406 pKa = 11.27VMNILRR412 pKa = 11.84MAGYY416 pKa = 9.64DD417 pKa = 3.41ATGGSCYY424 pKa = 10.57EE425 pKa = 4.02GDD427 pKa = 4.58KK428 pKa = 10.51VKK430 pKa = 10.83NWADD434 pKa = 3.15NSSGHH439 pKa = 5.97FLYY442 pKa = 10.33TRR444 pKa = 11.84SAEE447 pKa = 4.22APDD450 pKa = 5.03DD451 pKa = 4.76DD452 pKa = 4.97IFYY455 pKa = 10.99VDD457 pKa = 4.68LGLEE461 pKa = 4.22EE462 pKa = 5.04RR463 pKa = 11.84SKK465 pKa = 11.08SWVDD469 pKa = 3.47EE470 pKa = 4.22PRR472 pKa = 11.84WSEE475 pKa = 4.1KK476 pKa = 10.34ACLRR480 pKa = 11.84SEE482 pKa = 4.35VKK484 pKa = 9.04ITAFTMFKK492 pKa = 10.62DD493 pKa = 3.26GVMYY497 pKa = 10.9KK498 pKa = 10.63NITGVLKK505 pKa = 10.43KK506 pKa = 10.83YY507 pKa = 9.97SFKK510 pKa = 10.88QPSLEE515 pKa = 3.82ITSKK519 pKa = 8.75HH520 pKa = 3.92TRR522 pKa = 11.84EE523 pKa = 4.19VKK525 pKa = 9.11VTLRR529 pKa = 11.84PRR531 pKa = 11.84PHH533 pKa = 6.43IKK535 pKa = 10.29AGFRR539 pKa = 11.84MARR542 pKa = 11.84IDD544 pKa = 4.51DD545 pKa = 3.85RR546 pKa = 11.84TILHH550 pKa = 6.83PVTNTLFGDD559 pKa = 4.45LAQSDD564 pKa = 4.66EE565 pKa = 4.03QLDD568 pKa = 3.96WYY570 pKa = 10.65DD571 pKa = 3.4QDD573 pKa = 4.83QEE575 pKa = 4.78DD576 pKa = 4.24GTGAA580 pKa = 3.47

Molecular weight:
66.1 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S3Q2X1|A0A0S3Q2X1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 2 OX=1714363 GN=RdRp PE=3 SV=1
MM1 pKa = 7.82LITVYY6 pKa = 10.77ILMGLRR12 pKa = 11.84TRR14 pKa = 11.84GAVDD18 pKa = 3.26EE19 pKa = 4.79ALLNVKK25 pKa = 9.57ALHH28 pKa = 6.45KK29 pKa = 10.8LHH31 pKa = 6.07GQYY34 pKa = 11.01VLAIPVLFGNVIAYY48 pKa = 9.61YY49 pKa = 10.23VDD51 pKa = 3.15KK52 pKa = 10.99HH53 pKa = 6.43DD54 pKa = 3.84VTMADD59 pKa = 3.36KK60 pKa = 10.74KK61 pKa = 9.99VAKK64 pKa = 9.89QAGLVIANNVFVDD77 pKa = 4.74FCPDD81 pKa = 2.84ARR83 pKa = 11.84VDD85 pKa = 3.84YY86 pKa = 10.22IAVMGNMAKK95 pKa = 10.36GLHH98 pKa = 6.04KK99 pKa = 9.66KK100 pKa = 7.74TRR102 pKa = 11.84INDD105 pKa = 3.53RR106 pKa = 11.84EE107 pKa = 4.37LIRR110 pKa = 11.84SRR112 pKa = 11.84ISQRR116 pKa = 11.84HH117 pKa = 4.8HH118 pKa = 4.37MHH120 pKa = 6.46IKK122 pKa = 10.42VGMLDD127 pKa = 3.57SEE129 pKa = 4.33GDD131 pKa = 3.59EE132 pKa = 4.96NEE134 pKa = 4.16IEE136 pKa = 4.01LDD138 pKa = 3.75GVKK141 pKa = 10.43GYY143 pKa = 10.65IMHH146 pKa = 7.71RR147 pKa = 11.84LMKK150 pKa = 8.33MTTVRR155 pKa = 11.84QTVTTFLLYY164 pKa = 10.77VEE166 pKa = 5.6LAPSWAADD174 pKa = 3.41VLAVIVSRR182 pKa = 11.84HH183 pKa = 5.1SEE185 pKa = 3.67WAQLMDD191 pKa = 4.26DD192 pKa = 4.49LKK194 pKa = 11.09LFSMYY199 pKa = 10.01VKK201 pKa = 10.35QYY203 pKa = 8.45QHH205 pKa = 6.85VIRR208 pKa = 11.84SDD210 pKa = 3.53LAVLFEE216 pKa = 5.2LNVLANRR223 pKa = 11.84SDD225 pKa = 4.94SEE227 pKa = 4.22MDD229 pKa = 2.96WAADD233 pKa = 3.0IAKK236 pKa = 8.28RR237 pKa = 11.84TKK239 pKa = 10.68SVVATPDD246 pKa = 3.07QEE248 pKa = 4.24RR249 pKa = 11.84LYY251 pKa = 10.8RR252 pKa = 11.84DD253 pKa = 3.66GVSMFTSARR262 pKa = 11.84AEE264 pKa = 4.22GKK266 pKa = 10.13QPVTMKK272 pKa = 9.93WEE274 pKa = 4.57EE275 pKa = 4.0YY276 pKa = 10.98RR277 pKa = 11.84NMTWGLTPPGAVHH290 pKa = 6.61SPEE293 pKa = 3.71GDD295 pKa = 3.14FRR297 pKa = 11.84RR298 pKa = 11.84NISTIPSKK306 pKa = 10.83LRR308 pKa = 11.84NKK310 pKa = 8.61KK311 pKa = 8.27TYY313 pKa = 10.79NSVFRR318 pKa = 11.84PKK320 pKa = 10.26EE321 pKa = 3.79LKK323 pKa = 10.2HH324 pKa = 7.45YY325 pKa = 7.33MAQRR329 pKa = 11.84PKK331 pKa = 10.3IIAKK335 pKa = 10.06PSVKK339 pKa = 10.66YY340 pKa = 9.97EE341 pKa = 3.7WGKK344 pKa = 9.93NRR346 pKa = 11.84ALYY349 pKa = 10.66GCDD352 pKa = 3.4LVSHH356 pKa = 6.54MMTDD360 pKa = 3.87FGMMQAEE367 pKa = 4.39STLPSWMPTGRR378 pKa = 11.84MADD381 pKa = 3.48EE382 pKa = 4.27NRR384 pKa = 11.84VKK386 pKa = 10.68RR387 pKa = 11.84QIATMGNGIPFCYY400 pKa = 10.51DD401 pKa = 2.8FDD403 pKa = 4.94DD404 pKa = 4.89FNSQHH409 pKa = 5.81SVKK412 pKa = 10.75SMQTVITAWYY422 pKa = 9.13SVYY425 pKa = 11.08GDD427 pKa = 5.26LISDD431 pKa = 3.84EE432 pKa = 4.44QKK434 pKa = 11.15LCVDD438 pKa = 4.04WVVQSLEE445 pKa = 3.92TQLIINSVGLGSSKK459 pKa = 10.72YY460 pKa = 9.92IEE462 pKa = 3.96VDD464 pKa = 3.04GTLFSGWRR472 pKa = 11.84LTSFMNTCLNYY483 pKa = 10.7LYY485 pKa = 10.73LEE487 pKa = 4.19QANVTKK493 pKa = 9.94RR494 pKa = 11.84TVGNIHH500 pKa = 6.59NGDD503 pKa = 3.51DD504 pKa = 3.49VYY506 pKa = 11.99ANIRR510 pKa = 11.84TIGDD514 pKa = 3.59ALDD517 pKa = 3.18IMRR520 pKa = 11.84DD521 pKa = 3.35AKK523 pKa = 11.03SIGVRR528 pKa = 11.84AQMTKK533 pKa = 10.09TNIGTIGEE541 pKa = 4.27FLRR544 pKa = 11.84IDD546 pKa = 3.84NKK548 pKa = 11.13AVDD551 pKa = 3.8ATGAQYY557 pKa = 9.18LTRR560 pKa = 11.84SCATAVHH567 pKa = 6.17GRR569 pKa = 11.84IEE571 pKa = 4.56SDD573 pKa = 3.46TPNSTRR579 pKa = 11.84SVVEE583 pKa = 3.57ANMIRR588 pKa = 11.84LEE590 pKa = 3.96ALKK593 pKa = 10.92ARR595 pKa = 11.84GGDD598 pKa = 3.56SNKK601 pKa = 10.16IKK603 pKa = 10.54VIKK606 pKa = 10.44DD607 pKa = 3.26NMISRR612 pKa = 11.84IARR615 pKa = 11.84KK616 pKa = 9.32HH617 pKa = 5.31DD618 pKa = 3.35TSEE621 pKa = 3.78EE622 pKa = 4.39TICDD626 pKa = 4.3LIEE629 pKa = 4.14TPKK632 pKa = 11.18ALGGLKK638 pKa = 10.1DD639 pKa = 4.23DD640 pKa = 4.78NKK642 pKa = 9.95PVPWRR647 pKa = 11.84LIIKK651 pKa = 10.5LEE653 pKa = 4.22GEE655 pKa = 4.51LDD657 pKa = 3.51SMAYY661 pKa = 9.87EE662 pKa = 4.93LANKK666 pKa = 7.91MKK668 pKa = 10.53KK669 pKa = 9.93AVNNYY674 pKa = 8.66HH675 pKa = 7.2KK676 pKa = 10.31AVCDD680 pKa = 3.59MLEE683 pKa = 4.02VEE685 pKa = 4.97MPTGYY690 pKa = 11.41DD691 pKa = 3.05MLNGINEE698 pKa = 4.19GMLQTKK704 pKa = 9.7KK705 pKa = 9.91MRR707 pKa = 11.84IEE709 pKa = 4.37KK710 pKa = 10.23IKK712 pKa = 10.52IDD714 pKa = 3.3IKK716 pKa = 10.64LAARR720 pKa = 11.84ICAMHH725 pKa = 6.6KK726 pKa = 10.19AWGGSGDD733 pKa = 3.65GGMAATMRR741 pKa = 11.84IFTDD745 pKa = 2.66IKK747 pKa = 8.12MHH749 pKa = 6.16RR750 pKa = 11.84KK751 pKa = 8.74HH752 pKa = 6.67RR753 pKa = 11.84RR754 pKa = 11.84LRR756 pKa = 11.84DD757 pKa = 3.26QVRR760 pKa = 11.84RR761 pKa = 11.84LVKK764 pKa = 10.76SGDD767 pKa = 3.2FNLWFNTVYY776 pKa = 11.07

Molecular weight:
87.98 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1356

580

776

678.0

77.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.932 ± 0.477

1.18 ± 0.018

7.153 ± 0.285

5.383 ± 0.542

3.171 ± 0.636

6.047 ± 0.235

2.581 ± 0.11

6.121 ± 0.511

6.268 ± 0.948

8.776 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.499 ± 0.691

4.794 ± 0.091

3.171 ± 0.523

2.876 ± 0.036

5.9 ± 0.479

5.973 ± 0.381

6.195 ± 0.235

7.227 ± 0.331

1.549 ± 0.111

4.204 ± 0.638

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski