bacterium HR33

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6A6T8|A0A2H6A6T8_9BACT FHA domain-containing protein FhaB OS=bacterium HR33 OX=2035428 GN=fhaB PE=4 SV=1
MM1 pKa = 7.33SLAAEE6 pKa = 4.56CPEE9 pKa = 4.22CAAKK13 pKa = 10.12IALNDD18 pKa = 3.78DD19 pKa = 4.23VISGEE24 pKa = 4.0IVQCGDD30 pKa = 3.52CGLEE34 pKa = 4.06LEE36 pKa = 4.62VVRR39 pKa = 11.84DD40 pKa = 3.62GSVTLRR46 pKa = 11.84PAPEE50 pKa = 4.13EE51 pKa = 3.99EE52 pKa = 4.4EE53 pKa = 4.33DD54 pKa = 3.25WGEE57 pKa = 3.78

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6A5N4|A0A2H6A5N4_9BACT Segregation and condensation protein A OS=bacterium HR33 OX=2035428 GN=scpA_3 PE=4 SV=1
MM1 pKa = 7.4AVSSATRR8 pKa = 11.84PFNKK12 pKa = 9.58KK13 pKa = 9.71ARR15 pKa = 11.84IRR17 pKa = 11.84AGPATPAAIPVSTKK31 pKa = 9.95IPAPIIAPTPIMVMSMSRR49 pKa = 11.84ISRR52 pKa = 11.84LRR54 pKa = 11.84RR55 pKa = 11.84TSTATAA61 pKa = 3.65

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2661

0

2661

916511

29

1969

344.4

37.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.762 ± 0.066

0.764 ± 0.017

4.864 ± 0.033

6.792 ± 0.056

3.478 ± 0.028

8.84 ± 0.045

1.776 ± 0.02

4.429 ± 0.031

2.523 ± 0.029

10.823 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.019

2.125 ± 0.029

5.65 ± 0.033

2.936 ± 0.024

9.021 ± 0.044

5.291 ± 0.031

4.699 ± 0.035

8.344 ± 0.041

1.563 ± 0.022

2.556 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski