Mycobacterium phage SgtBeansprout

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N3T3|A0A2P1N3T3_9CAUD Uncharacterized protein OS=Mycobacterium phage SgtBeansprout OX=2126788 GN=71 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 5.43APTHH6 pKa = 5.1FTAVASTGDD15 pKa = 3.51EE16 pKa = 4.33PGWLLVHH23 pKa = 6.26VPEE26 pKa = 5.77IDD28 pKa = 3.29QYY30 pKa = 9.19TQARR34 pKa = 11.84TLDD37 pKa = 3.64EE38 pKa = 4.8VGPMAADD45 pKa = 6.26LIATWLNVPVEE56 pKa = 4.4SVDD59 pKa = 4.08VEE61 pKa = 4.63VKK63 pKa = 9.71YY64 pKa = 9.62TDD66 pKa = 3.01

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N3S2|A0A2P1N3S2_9CAUD Uncharacterized protein OS=Mycobacterium phage SgtBeansprout OX=2126788 GN=70 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.22RR3 pKa = 11.84MKK5 pKa = 10.33AFRR8 pKa = 11.84SSVPPVPQPEE18 pKa = 4.28VVVNGRR24 pKa = 11.84VLAPGTEE31 pKa = 3.94VSIRR35 pKa = 11.84GEE37 pKa = 3.63RR38 pKa = 11.84GRR40 pKa = 11.84FRR42 pKa = 11.84FVKK45 pKa = 10.45AAVTSAGRR53 pKa = 11.84ITCDD57 pKa = 5.22FIGPDD62 pKa = 4.59DD63 pKa = 3.9NTKK66 pKa = 10.01CWRR69 pKa = 11.84SFYY72 pKa = 9.91PEE74 pKa = 4.32RR75 pKa = 11.84IKK77 pKa = 10.01TVHH80 pKa = 5.78RR81 pKa = 11.84VNRR84 pKa = 11.84TRR86 pKa = 11.84ANVAA90 pKa = 3.07

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

17391

39

1377

223.0

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.731 ± 0.498

1.11 ± 0.142

6.463 ± 0.253

6.107 ± 0.36

2.645 ± 0.152

8.683 ± 0.54

2.214 ± 0.178

3.755 ± 0.171

3.197 ± 0.224

8.28 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.093 ± 0.107

2.783 ± 0.253

5.589 ± 0.242

3.232 ± 0.214

7.533 ± 0.4

5.215 ± 0.235

5.572 ± 0.197

8.32 ± 0.356

2.162 ± 0.131

2.317 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski