Klebsiella phage 2 LV-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2GUA0|A0A2Z2GUA0_9CAUD Uncharacterized protein OS=Klebsiella phage 2 LV-2017 OX=1960655 PE=4 SV=1
MM1 pKa = 7.85PIDD4 pKa = 4.65LGDD7 pKa = 3.7TQGVQQQTLHH17 pKa = 5.86FVDD20 pKa = 4.76MPVQTSKK27 pKa = 11.19NGNIVTWTATVISNGIEE44 pKa = 4.99DD45 pKa = 3.49ITEE48 pKa = 5.19DD49 pKa = 3.7YY50 pKa = 11.32DD51 pKa = 3.6DD52 pKa = 5.38WIVEE56 pKa = 4.09AQPGYY61 pKa = 10.94GNWLDD66 pKa = 3.8YY67 pKa = 11.2LITEE71 pKa = 4.13VMPRR75 pKa = 11.84ADD77 pKa = 3.12

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2GNA8|A0A2Z2GNA8_9CAUD Uncharacterized protein OS=Klebsiella phage 2 LV-2017 OX=1960655 PE=4 SV=1
MM1 pKa = 6.98GVKK4 pKa = 9.98VRR6 pKa = 11.84GIRR9 pKa = 11.84QAKK12 pKa = 9.77ANLDD16 pKa = 4.05RR17 pKa = 11.84IIKK20 pKa = 9.58DD21 pKa = 3.25VQGRR25 pKa = 11.84KK26 pKa = 7.14VVRR29 pKa = 11.84AIQSAMLIGSAQAALYY45 pKa = 9.29TPIDD49 pKa = 3.7TSTLINSQFRR59 pKa = 11.84EE60 pKa = 3.84ITANGVRR67 pKa = 11.84VTGRR71 pKa = 11.84VGYY74 pKa = 6.19TANYY78 pKa = 9.91AVFVQDD84 pKa = 4.98PEE86 pKa = 4.36VKK88 pKa = 10.26QNFRR92 pKa = 11.84RR93 pKa = 11.84ATARR97 pKa = 11.84KK98 pKa = 8.77EE99 pKa = 3.61FLTKK103 pKa = 10.63GFEE106 pKa = 4.15DD107 pKa = 3.5TRR109 pKa = 11.84NQIDD113 pKa = 3.58AAVKK117 pKa = 10.59KK118 pKa = 10.05EE119 pKa = 3.74LSLL122 pKa = 4.3

Molecular weight: 13.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10989

56

1121

219.8

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.984 ± 0.709

1.283 ± 0.202

5.942 ± 0.381

6.689 ± 0.354

3.358 ± 0.218

7.253 ± 0.33

1.674 ± 0.211

5.078 ± 0.174

5.415 ± 0.376

7.417 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.2

4.596 ± 0.266

3.804 ± 0.318

4.641 ± 0.484

6.152 ± 0.317

5.997 ± 0.31

5.515 ± 0.381

6.661 ± 0.315

1.984 ± 0.184

2.93 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski