Rhizobium sp. RU20A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6NJF9|A0A1N6NJF9_9RHIZ L-aspartate oxidase OS=Rhizobium sp. RU20A OX=1907412 GN=SAMN05880582_10128 PE=3 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.32PEE32 pKa = 4.04PVEE35 pKa = 4.04YY36 pKa = 11.09VRR38 pKa = 11.84VCDD41 pKa = 4.56AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.83TCLKK58 pKa = 8.97IGGFIRR64 pKa = 11.84VQTNFGPNASGTSDD78 pKa = 2.86WDD80 pKa = 3.8SFSRR84 pKa = 11.84AYY86 pKa = 9.87ISFDD90 pKa = 3.01AKK92 pKa = 11.03SDD94 pKa = 3.79TEE96 pKa = 4.22YY97 pKa = 9.93GTLTGFFAAEE107 pKa = 3.95FNADD111 pKa = 3.21NDD113 pKa = 4.36SAVGDD118 pKa = 3.94SLIDD122 pKa = 3.26VDD124 pKa = 4.33EE125 pKa = 5.4AYY127 pKa = 9.83IQLGGLKK134 pKa = 10.48AGFFYY139 pKa = 10.86NWWDD143 pKa = 3.2KK144 pKa = 11.3GINGEE149 pKa = 4.23TDD151 pKa = 3.03TLGG154 pKa = 3.48

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6TYR5|A0A1N6TYR5_9RHIZ dTTP/UTP pyrophosphatase OS=Rhizobium sp. RU20A OX=1907412 GN=SAMN05880582_102202 PE=3 SV=1
MM1 pKa = 7.55IVLTVTAALLAMLSAIAIGIHH22 pKa = 6.09AGVARR27 pKa = 11.84RR28 pKa = 11.84PRR30 pKa = 11.84RR31 pKa = 11.84AGRR34 pKa = 11.84LPFF37 pKa = 4.48

Molecular weight: 3.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4181

0

4181

1320648

29

2830

315.9

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.71 ± 0.055

0.783 ± 0.011

5.762 ± 0.027

5.756 ± 0.039

3.804 ± 0.026

8.504 ± 0.047

2.003 ± 0.018

5.459 ± 0.029

3.434 ± 0.032

9.98 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.019

2.568 ± 0.021

4.992 ± 0.028

2.959 ± 0.026

6.984 ± 0.048

5.423 ± 0.027

5.593 ± 0.032

7.349 ± 0.031

1.182 ± 0.017

2.19 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski