Vibrio phage VMJ710

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LS18|A0A6B9LS18_9CAUD Uncharacterized protein OS=Vibrio phage VMJ710 OX=2595040 PE=4 SV=1
MM1 pKa = 7.2VFVGLKK7 pKa = 10.35CFTNKK12 pKa = 10.41GEE14 pKa = 4.5IIIMQNNIDD23 pKa = 3.61VDD25 pKa = 4.32TNEE28 pKa = 4.81HH29 pKa = 6.06LNSRR33 pKa = 11.84YY34 pKa = 9.24IQSFLDD40 pKa = 3.59EE41 pKa = 4.26YY42 pKa = 11.44GFYY45 pKa = 10.9DD46 pKa = 4.02NEE48 pKa = 4.22IFDD51 pKa = 5.0GEE53 pKa = 4.37NFCIWMEE60 pKa = 4.04EE61 pKa = 4.02MM62 pKa = 5.21

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LKS5|A0A6B9LKS5_9CAUD Terminase large subunit OS=Vibrio phage VMJ710 OX=2595040 PE=4 SV=1
MM1 pKa = 7.69LRR3 pKa = 11.84RR4 pKa = 11.84QILDD8 pKa = 3.75VIDD11 pKa = 3.77VLVDD15 pKa = 3.46GKK17 pKa = 10.99FVLEE21 pKa = 4.69LKK23 pKa = 8.92NTNLKK28 pKa = 9.1WRR30 pKa = 11.84GSSNQRR36 pKa = 11.84VWKK39 pKa = 10.12LNIMM43 pKa = 4.25

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

23724

34

1076

266.6

30.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.1 ± 0.287

1.737 ± 0.138

6.196 ± 0.171

7.465 ± 0.22

4.375 ± 0.156

6.521 ± 0.2

1.914 ± 0.097

6.154 ± 0.164

8.051 ± 0.273

8.38 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.143

5.21 ± 0.193

3.166 ± 0.117

3.499 ± 0.162

4.354 ± 0.162

7.065 ± 0.158

5.699 ± 0.257

6.803 ± 0.204

1.399 ± 0.104

4.527 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski