Amino acid dipepetide frequency for Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.942AlaAla: 2.942 ± 0.093
0.352AlaCys: 0.352 ± 0.028
2.268AlaAsp: 2.268 ± 0.072
3.599AlaGlu: 3.599 ± 0.095
2.146AlaPhe: 2.146 ± 0.067
3.337AlaGly: 3.337 ± 0.088
0.808AlaHis: 0.808 ± 0.04
8.606AlaIle: 8.606 ± 0.157
4.226AlaLys: 4.226 ± 0.111
7.403AlaLeu: 7.403 ± 0.135
1.671AlaMet: 1.671 ± 0.062
1.839AlaAsn: 1.839 ± 0.053
1.991AlaPro: 1.991 ± 0.064
1.107AlaGln: 1.107 ± 0.05
3.701AlaArg: 3.701 ± 0.082
4.025AlaSer: 4.025 ± 0.088
3.091AlaThr: 3.091 ± 0.094
4.367AlaVal: 4.367 ± 0.089
0.612AlaTrp: 0.612 ± 0.036
2.447AlaTyr: 2.447 ± 0.076
0.0AlaXaa: 0.0 ± 0.0
Cys
0.348CysAla: 0.348 ± 0.027
0.138CysCys: 0.138 ± 0.018
0.408CysAsp: 0.408 ± 0.028
0.448CysGlu: 0.448 ± 0.032
0.326CysPhe: 0.326 ± 0.025
0.899CysGly: 0.899 ± 0.046
0.125CysHis: 0.125 ± 0.015
1.143CysIle: 1.143 ± 0.05
0.493CysLys: 0.493 ± 0.03
0.692CysLeu: 0.692 ± 0.034
0.184CysMet: 0.184 ± 0.02
0.409CysAsn: 0.409 ± 0.027
0.616CysPro: 0.616 ± 0.044
0.125CysGln: 0.125 ± 0.016
0.765CysArg: 0.765 ± 0.038
0.748CysSer: 0.748 ± 0.042
0.361CysThr: 0.361 ± 0.028
0.432CysVal: 0.432 ± 0.033
0.121CysTrp: 0.121 ± 0.016
0.355CysTyr: 0.355 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
2.603AspAla: 2.603 ± 0.069
0.283AspCys: 0.283 ± 0.025
2.138AspAsp: 2.138 ± 0.065
3.984AspGlu: 3.984 ± 0.102
1.785AspPhe: 1.785 ± 0.052
2.721AspGly: 2.721 ± 0.078
0.631AspHis: 0.631 ± 0.031
7.901AspIle: 7.901 ± 0.143
2.968AspLys: 2.968 ± 0.089
4.795AspLeu: 4.795 ± 0.094
1.308AspMet: 1.308 ± 0.051
1.814AspAsn: 1.814 ± 0.061
2.562AspPro: 2.562 ± 0.071
0.618AspGln: 0.618 ± 0.035
3.591AspArg: 3.591 ± 0.089
2.702AspSer: 2.702 ± 0.078
2.095AspThr: 2.095 ± 0.07
4.73AspVal: 4.73 ± 0.093
0.553AspTrp: 0.553 ± 0.034
2.162AspTyr: 2.162 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
4.559GluAla: 4.559 ± 0.097
0.586GluCys: 0.586 ± 0.034
3.454GluAsp: 3.454 ± 0.078
5.715GluGlu: 5.715 ± 0.139
2.534GluPhe: 2.534 ± 0.07
3.372GluGly: 3.372 ± 0.094
0.901GluHis: 0.901 ± 0.046
7.475GluIle: 7.475 ± 0.125
5.294GluLys: 5.294 ± 0.105
7.295GluLeu: 7.295 ± 0.143
1.422GluMet: 1.422 ± 0.065
2.977GluAsn: 2.977 ± 0.082
2.307GluPro: 2.307 ± 0.064
1.102GluGln: 1.102 ± 0.045
3.919GluArg: 3.919 ± 0.097
3.368GluSer: 3.368 ± 0.083
2.616GluThr: 2.616 ± 0.078
4.855GluVal: 4.855 ± 0.11
0.709GluTrp: 0.709 ± 0.038
3.271GluTyr: 3.271 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
2.002PheAla: 2.002 ± 0.063
0.303PheCys: 0.303 ± 0.025
1.868PheAsp: 1.868 ± 0.062
2.224PheGlu: 2.224 ± 0.065
1.176PhePhe: 1.176 ± 0.05
2.328PheGly: 2.328 ± 0.065
0.56PheHis: 0.56 ± 0.031
4.464PheIle: 4.464 ± 0.103
1.794PheLys: 1.794 ± 0.062
3.498PheLeu: 3.498 ± 0.097
0.839PheMet: 0.839 ± 0.041
1.677PheAsn: 1.677 ± 0.054
1.144PhePro: 1.144 ± 0.046
0.633PheGln: 0.633 ± 0.035
2.432PheArg: 2.432 ± 0.071
2.724PheSer: 2.724 ± 0.069
1.844PheThr: 1.844 ± 0.065
2.523PheVal: 2.523 ± 0.068
0.367PheTrp: 0.367 ± 0.024
1.692PheTyr: 1.692 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
3.491GlyAla: 3.491 ± 0.097
0.666GlyCys: 0.666 ± 0.037
3.061GlyAsp: 3.061 ± 0.076
3.765GlyGlu: 3.765 ± 0.095
3.063GlyPhe: 3.063 ± 0.082
4.088GlyGly: 4.088 ± 0.112
0.947GlyHis: 0.947 ± 0.045
7.641GlyIle: 7.641 ± 0.136
3.651GlyLys: 3.651 ± 0.085
5.698GlyLeu: 5.698 ± 0.115
1.468GlyMet: 1.468 ± 0.054
2.086GlyAsn: 2.086 ± 0.069
1.591GlyPro: 1.591 ± 0.06
0.832GlyGln: 0.832 ± 0.042
3.856GlyArg: 3.856 ± 0.082
3.915GlySer: 3.915 ± 0.097
2.468GlyThr: 2.468 ± 0.069
5.207GlyVal: 5.207 ± 0.107
0.731GlyTrp: 0.731 ± 0.036
3.753GlyTyr: 3.753 ± 0.091
0.0GlyXaa: 0.0 ± 0.0
His
0.709HisAla: 0.709 ± 0.04
0.16HisCys: 0.16 ± 0.019
0.674HisAsp: 0.674 ± 0.035
0.921HisGlu: 0.921 ± 0.041
0.402HisPhe: 0.402 ± 0.027
1.215HisGly: 1.215 ± 0.05
0.287HisHis: 0.287 ± 0.022
1.876HisIle: 1.876 ± 0.066
0.573HisLys: 0.573 ± 0.033
1.256HisLeu: 1.256 ± 0.051
0.411HisMet: 0.411 ± 0.024
0.482HisAsn: 0.482 ± 0.033
0.806HisPro: 0.806 ± 0.039
0.257HisGln: 0.257 ± 0.022
1.327HisArg: 1.327 ± 0.052
0.901HisSer: 0.901 ± 0.043
0.685HisThr: 0.685 ± 0.031
1.048HisVal: 1.048 ± 0.045
0.188HisTrp: 0.188 ± 0.019
0.512HisTyr: 0.512 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
9.993IleAla: 9.993 ± 0.169
1.117IleCys: 1.117 ± 0.045
8.759IleAsp: 8.759 ± 0.133
9.061IleGlu: 9.061 ± 0.148
3.768IlePhe: 3.768 ± 0.095
7.481IleGly: 7.481 ± 0.145
1.762IleHis: 1.762 ± 0.07
9.178IleIle: 9.178 ± 0.163
5.491IleLys: 5.491 ± 0.113
10.123IleLeu: 10.123 ± 0.152
2.136IleMet: 2.136 ± 0.063
4.395IleAsn: 4.395 ± 0.085
5.043IlePro: 5.043 ± 0.105
1.846IleGln: 1.846 ± 0.056
5.702IleArg: 5.702 ± 0.108
7.706IleSer: 7.706 ± 0.138
4.572IleThr: 4.572 ± 0.111
10.186IleVal: 10.186 ± 0.15
0.869IleTrp: 0.869 ± 0.046
5.452IleTyr: 5.452 ± 0.126
0.0IleXaa: 0.0 ± 0.0
Lys
3.815LysAla: 3.815 ± 0.099
0.692LysCys: 0.692 ± 0.035
2.78LysAsp: 2.78 ± 0.077
4.096LysGlu: 4.096 ± 0.111
1.753LysPhe: 1.753 ± 0.059
3.284LysGly: 3.284 ± 0.081
0.971LysHis: 0.971 ± 0.04
6.751LysIle: 6.751 ± 0.129
3.564LysLys: 3.564 ± 0.096
6.109LysLeu: 6.109 ± 0.129
1.639LysMet: 1.639 ± 0.061
2.52LysAsn: 2.52 ± 0.089
2.804LysPro: 2.804 ± 0.076
1.163LysGln: 1.163 ± 0.047
3.688LysArg: 3.688 ± 0.092
3.476LysSer: 3.476 ± 0.076
3.044LysThr: 3.044 ± 0.076
3.724LysVal: 3.724 ± 0.092
0.569LysTrp: 0.569 ± 0.036
2.693LysTyr: 2.693 ± 0.082
0.0LysXaa: 0.0 ± 0.0
Leu
6.426LeuAla: 6.426 ± 0.118
0.916LeuCys: 0.916 ± 0.044
5.309LeuAsp: 5.309 ± 0.103
6.708LeuGlu: 6.708 ± 0.142
3.43LeuPhe: 3.43 ± 0.092
6.643LeuGly: 6.643 ± 0.127
1.515LeuHis: 1.515 ± 0.058
8.387LeuIle: 8.387 ± 0.145
6.433LeuLys: 6.433 ± 0.114
9.479LeuLeu: 9.479 ± 0.163
2.207LeuMet: 2.207 ± 0.07
4.124LeuAsn: 4.124 ± 0.09
3.606LeuPro: 3.606 ± 0.077
1.95LeuGln: 1.95 ± 0.063
6.682LeuArg: 6.682 ± 0.121
7.741LeuSer: 7.741 ± 0.147
4.111LeuThr: 4.111 ± 0.097
6.085LeuVal: 6.085 ± 0.097
1.022LeuTrp: 1.022 ± 0.048
4.756LeuTyr: 4.756 ± 0.1
0.0LeuXaa: 0.0 ± 0.0
Met
1.589MetAla: 1.589 ± 0.054
0.233MetCys: 0.233 ± 0.02
1.384MetAsp: 1.384 ± 0.053
1.358MetGlu: 1.358 ± 0.055
0.977MetPhe: 0.977 ± 0.04
1.621MetGly: 1.621 ± 0.064
0.361MetHis: 0.361 ± 0.026
2.0MetIle: 2.0 ± 0.061
1.558MetLys: 1.558 ± 0.062
2.406MetLeu: 2.406 ± 0.083
0.489MetMet: 0.489 ± 0.03
1.115MetAsn: 1.115 ± 0.049
0.984MetPro: 0.984 ± 0.043
0.35MetGln: 0.35 ± 0.026
1.304MetArg: 1.304 ± 0.052
1.781MetSer: 1.781 ± 0.059
0.875MetThr: 0.875 ± 0.039
1.917MetVal: 1.917 ± 0.058
0.242MetTrp: 0.242 ± 0.024
1.12MetTyr: 1.12 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
2.114AsnAla: 2.114 ± 0.059
0.331AsnCys: 0.331 ± 0.025
1.649AsnAsp: 1.649 ± 0.053
2.315AsnGlu: 2.315 ± 0.069
1.066AsnPhe: 1.066 ± 0.054
2.257AsnGly: 2.257 ± 0.07
0.478AsnHis: 0.478 ± 0.03
6.319AsnIle: 6.319 ± 0.131
1.807AsnLys: 1.807 ± 0.06
3.562AsnLeu: 3.562 ± 0.093
1.083AsnMet: 1.083 ± 0.05
1.978AsnAsn: 1.978 ± 0.085
2.14AsnPro: 2.14 ± 0.063
0.519AsnGln: 0.519 ± 0.03
2.544AsnArg: 2.544 ± 0.072
2.391AsnSer: 2.391 ± 0.077
2.168AsnThr: 2.168 ± 0.064
3.013AsnVal: 3.013 ± 0.08
0.328AsnTrp: 0.328 ± 0.024
1.491AsnTyr: 1.491 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
1.673ProAla: 1.673 ± 0.065
0.344ProCys: 0.344 ± 0.026
1.846ProAsp: 1.846 ± 0.066
2.503ProGlu: 2.503 ± 0.068
1.444ProPhe: 1.444 ± 0.049
2.352ProGly: 2.352 ± 0.079
0.685ProHis: 0.685 ± 0.035
4.565ProIle: 4.565 ± 0.095
2.274ProLys: 2.274 ± 0.074
3.798ProLeu: 3.798 ± 0.084
0.929ProMet: 0.929 ± 0.044
1.42ProAsn: 1.42 ± 0.051
2.012ProPro: 2.012 ± 0.07
1.027ProGln: 1.027 ± 0.057
2.616ProArg: 2.616 ± 0.071
3.056ProSer: 3.056 ± 0.085
2.123ProThr: 2.123 ± 0.073
2.257ProVal: 2.257 ± 0.068
0.635ProTrp: 0.635 ± 0.034
2.123ProTyr: 2.123 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
0.964GlnAla: 0.964 ± 0.045
0.181GlnCys: 0.181 ± 0.02
0.67GlnAsp: 0.67 ± 0.036
1.09GlnGlu: 1.09 ± 0.041
0.607GlnPhe: 0.607 ± 0.033
1.038GlnGly: 1.038 ± 0.047
0.355GlnHis: 0.355 ± 0.026
1.852GlnIle: 1.852 ± 0.056
1.094GlnLys: 1.094 ± 0.045
1.911GlnLeu: 1.911 ± 0.066
0.486GlnMet: 0.486 ± 0.033
0.726GlnAsn: 0.726 ± 0.035
0.687GlnPro: 0.687 ± 0.048
0.662GlnGln: 0.662 ± 0.048
1.239GlnArg: 1.239 ± 0.046
1.023GlnSer: 1.023 ± 0.048
0.852GlnThr: 0.852 ± 0.045
0.91GlnVal: 0.91 ± 0.045
0.22GlnTrp: 0.22 ± 0.018
0.765GlnTyr: 0.765 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.629ArgAla: 3.629 ± 0.105
0.795ArgCys: 0.795 ± 0.046
4.144ArgAsp: 4.144 ± 0.1
5.08ArgGlu: 5.08 ± 0.1
2.709ArgPhe: 2.709 ± 0.084
4.267ArgGly: 4.267 ± 0.105
0.936ArgHis: 0.936 ± 0.048
6.645ArgIle: 6.645 ± 0.112
3.841ArgLys: 3.841 ± 0.095
5.369ArgLeu: 5.369 ± 0.113
1.37ArgMet: 1.37 ± 0.049
2.722ArgAsn: 2.722 ± 0.07
1.831ArgPro: 1.831 ± 0.056
0.908ArgGln: 0.908 ± 0.04
4.079ArgArg: 4.079 ± 0.099
3.247ArgSer: 3.247 ± 0.08
2.015ArgThr: 2.015 ± 0.061
4.699ArgVal: 4.699 ± 0.097
0.733ArgTrp: 0.733 ± 0.036
3.999ArgTyr: 3.999 ± 0.095
0.0ArgXaa: 0.0 ± 0.0
Ser
2.834SerAla: 2.834 ± 0.074
0.547SerCys: 0.547 ± 0.036
2.936SerAsp: 2.936 ± 0.08
3.703SerGlu: 3.703 ± 0.088
2.401SerPhe: 2.401 ± 0.065
4.027SerGly: 4.027 ± 0.098
0.873SerHis: 0.873 ± 0.041
9.628SerIle: 9.628 ± 0.181
4.07SerLys: 4.07 ± 0.085
6.656SerLeu: 6.656 ± 0.135
2.03SerMet: 2.03 ± 0.067
2.514SerAsn: 2.514 ± 0.079
2.484SerPro: 2.484 ± 0.074
1.334SerGln: 1.334 ± 0.054
4.367SerArg: 4.367 ± 0.093
4.983SerSer: 4.983 ± 0.104
3.238SerThr: 3.238 ± 0.083
3.699SerVal: 3.699 ± 0.088
0.724SerTrp: 0.724 ± 0.037
3.083SerTyr: 3.083 ± 0.088
0.0SerXaa: 0.0 ± 0.0
Thr
2.767ThrAla: 2.767 ± 0.07
0.4ThrCys: 0.4 ± 0.029
1.604ThrAsp: 1.604 ± 0.051
2.183ThrGlu: 2.183 ± 0.066
1.638ThrPhe: 1.638 ± 0.056
2.734ThrGly: 2.734 ± 0.083
0.782ThrHis: 0.782 ± 0.036
5.441ThrIle: 5.441 ± 0.121
2.421ThrLys: 2.421 ± 0.062
4.952ThrLeu: 4.952 ± 0.112
1.174ThrMet: 1.174 ± 0.055
1.608ThrAsn: 1.608 ± 0.061
2.417ThrPro: 2.417 ± 0.089
0.929ThrGln: 0.929 ± 0.051
2.082ThrArg: 2.082 ± 0.065
3.173ThrSer: 3.173 ± 0.089
3.175ThrThr: 3.175 ± 0.154
3.392ThrVal: 3.392 ± 0.101
0.471ThrTrp: 0.471 ± 0.028
2.077ThrTyr: 2.077 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
4.892ValAla: 4.892 ± 0.114
0.581ValCys: 0.581 ± 0.037
4.442ValAsp: 4.442 ± 0.096
5.588ValGlu: 5.588 ± 0.116
3.108ValPhe: 3.108 ± 0.08
4.012ValGly: 4.012 ± 0.109
0.949ValHis: 0.949 ± 0.043
7.313ValIle: 7.313 ± 0.123
4.987ValLys: 4.987 ± 0.101
7.043ValLeu: 7.043 ± 0.113
1.502ValMet: 1.502 ± 0.05
2.728ValAsn: 2.728 ± 0.078
2.449ValPro: 2.449 ± 0.068
1.04ValGln: 1.04 ± 0.046
4.185ValArg: 4.185 ± 0.097
4.976ValSer: 4.976 ± 0.091
3.041ValThr: 3.041 ± 0.088
6.431ValVal: 6.431 ± 0.127
0.718ValTrp: 0.718 ± 0.036
3.688ValTyr: 3.688 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.508TrpAla: 0.508 ± 0.034
0.104TrpCys: 0.104 ± 0.013
0.534TrpAsp: 0.534 ± 0.034
0.536TrpGlu: 0.536 ± 0.033
0.476TrpPhe: 0.476 ± 0.031
0.685TrpGly: 0.685 ± 0.038
0.203TrpHis: 0.203 ± 0.022
1.215TrpIle: 1.215 ± 0.053
0.545TrpLys: 0.545 ± 0.029
1.128TrpLeu: 1.128 ± 0.049
0.221TrpMet: 0.221 ± 0.019
0.571TrpAsn: 0.571 ± 0.033
0.316TrpPro: 0.316 ± 0.026
0.203TrpGln: 0.203 ± 0.021
0.865TrpArg: 0.865 ± 0.043
0.748TrpSer: 0.748 ± 0.043
0.398TrpThr: 0.398 ± 0.027
0.547TrpVal: 0.547 ± 0.036
0.156TrpTrp: 0.156 ± 0.023
0.534TrpTyr: 0.534 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.68TyrAla: 2.68 ± 0.07
0.408TyrCys: 0.408 ± 0.03
2.017TyrAsp: 2.017 ± 0.062
2.855TyrGlu: 2.855 ± 0.073
1.286TyrPhe: 1.286 ± 0.05
3.618TyrGly: 3.618 ± 0.087
0.56TyrHis: 0.56 ± 0.032
6.239TyrIle: 6.239 ± 0.125
2.053TyrLys: 2.053 ± 0.054
4.358TyrLeu: 4.358 ± 0.081
1.092TyrMet: 1.092 ± 0.051
1.848TyrAsn: 1.848 ± 0.066
1.889TyrPro: 1.889 ± 0.06
0.728TyrGln: 0.728 ± 0.039
4.072TyrArg: 4.072 ± 0.102
3.448TyrSer: 3.448 ± 0.084
2.646TyrThr: 2.646 ± 0.073
3.651TyrVal: 3.651 ± 0.084
0.528TyrTrp: 0.528 ± 0.037
1.641TyrTyr: 1.641 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1929 proteins (537386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski