Acinetobacter phage IMEAB3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lokivirus; Acinetobacter virus IMEAB3

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6B0X6|W6B0X6_9CAUD Uncharacterized protein OS=Acinetobacter phage IMEAB3 OX=1458669 GN=IME_AB3_55 PE=4 SV=1
MM1 pKa = 7.46SFYY4 pKa = 9.34EE5 pKa = 4.2TTVTFDD11 pKa = 3.14NGEE14 pKa = 4.16HH15 pKa = 5.88EE16 pKa = 5.11FEE18 pKa = 4.63LLLTGFAGNDD28 pKa = 3.43GGQSDD33 pKa = 4.7VEE35 pKa = 4.43GEE37 pKa = 4.07TTYY40 pKa = 10.9TIDD43 pKa = 3.94GDD45 pKa = 4.96DD46 pKa = 3.72YY47 pKa = 12.0ALDD50 pKa = 4.02EE51 pKa = 4.31LTEE54 pKa = 4.02EE55 pKa = 4.06QKK57 pKa = 11.2AIFFDD62 pKa = 4.04MEE64 pKa = 4.04LHH66 pKa = 5.9HH67 pKa = 6.44WTDD70 pKa = 2.25KK71 pKa = 10.65RR72 pKa = 11.84YY73 pKa = 9.67NVKK76 pKa = 9.33TEE78 pKa = 3.9YY79 pKa = 10.53PNTTKK84 pKa = 10.17WDD86 pKa = 3.83DD87 pKa = 3.17

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6AQL6|W6AQL6_9CAUD Putative recombinase OS=Acinetobacter phage IMEAB3 OX=1458669 GN=IME_AB3_14 PE=4 SV=1
MM1 pKa = 6.55YY2 pKa = 10.22QRR4 pKa = 11.84LEE6 pKa = 4.2GNSVVVSYY14 pKa = 8.88ITRR17 pKa = 11.84SLLLFGITDD26 pKa = 3.97RR27 pKa = 11.84CNVSLKK33 pKa = 10.23INHH36 pKa = 6.87TIHH39 pKa = 6.8TILLFTLTKK48 pKa = 10.37KK49 pKa = 9.23RR50 pKa = 11.84TSKK53 pKa = 10.85QITEE57 pKa = 3.98

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13620

40

1158

238.9

26.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.229 ± 0.765

0.999 ± 0.155

6.006 ± 0.25

6.079 ± 0.287

3.869 ± 0.191

7.232 ± 0.325

1.542 ± 0.122

6.351 ± 0.221

6.3 ± 0.335

7.584 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.141

5.543 ± 0.182

3.877 ± 0.277

4.236 ± 0.32

4.31 ± 0.213

5.852 ± 0.248

6.483 ± 0.281

6.564 ± 0.245

1.571 ± 0.12

3.678 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski