Gossypium raimondii (New World cotton)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66534 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2R2Q6|A0A0D2R2Q6_GOSRA Fe2OG dioxygenase domain-containing protein OS=Gossypium raimondii OX=29730 GN=B456_010G058200 PE=3 SV=1
MM1 pKa = 7.47EE2 pKa = 5.61NEE4 pKa = 4.34DD5 pKa = 4.01AKK7 pKa = 10.89QSSKK11 pKa = 10.59RR12 pKa = 11.84VPFTQLDD19 pKa = 3.71QVNSDD24 pKa = 3.1FAMAVALQEE33 pKa = 4.01QEE35 pKa = 4.3RR36 pKa = 11.84AFSMLEE42 pKa = 4.06SIEE45 pKa = 4.81SEE47 pKa = 3.96DD48 pKa = 3.63SEE50 pKa = 4.87EE51 pKa = 4.2YY52 pKa = 10.58NSEE55 pKa = 3.88SSYY58 pKa = 11.78EE59 pKa = 3.93EE60 pKa = 4.99GNVNDD65 pKa = 3.62YY66 pKa = 11.23EE67 pKa = 4.35YY68 pKa = 11.31FEE70 pKa = 4.5GLEE73 pKa = 4.14AGGDD77 pKa = 3.77LEE79 pKa = 4.35FLEE82 pKa = 5.09GQGSNDD88 pKa = 3.96DD89 pKa = 4.36EE90 pKa = 5.91DD91 pKa = 4.74MEE93 pKa = 7.51DD94 pKa = 6.1DD95 pKa = 5.64DD96 pKa = 7.1FEE98 pKa = 8.04DD99 pKa = 6.33DD100 pKa = 6.34DD101 pKa = 7.52DD102 pKa = 7.51DD103 pKa = 7.67DD104 pKa = 7.25DD105 pKa = 7.37DD106 pKa = 7.33DD107 pKa = 7.37GIDD110 pKa = 4.38PDD112 pKa = 4.0DD113 pKa = 4.36LSYY116 pKa = 11.8EE117 pKa = 4.01EE118 pKa = 5.78LIALGEE124 pKa = 4.12IIGVEE129 pKa = 4.0KK130 pKa = 10.75RR131 pKa = 11.84GLSPNEE137 pKa = 3.53ISSCLVRR144 pKa = 11.84CNFRR148 pKa = 11.84SDD150 pKa = 3.19EE151 pKa = 4.32CKK153 pKa = 10.25TGIDD157 pKa = 3.25RR158 pKa = 11.84CVICQVEE165 pKa = 4.27YY166 pKa = 10.87EE167 pKa = 4.19EE168 pKa = 4.73EE169 pKa = 4.17EE170 pKa = 4.66GVVALPNCEE179 pKa = 4.13HH180 pKa = 7.61PYY182 pKa = 10.31HH183 pKa = 6.79SEE185 pKa = 5.26CITKK189 pKa = 9.12WLQVKK194 pKa = 8.88KK195 pKa = 10.11ICPICSTEE203 pKa = 3.63ISSPKK208 pKa = 9.81NN209 pKa = 3.21

Molecular weight: 23.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2VUQ6|A0A0D2VUQ6_GOSRA X8 domain-containing protein OS=Gossypium raimondii OX=29730 GN=B456_012G041000 PE=4 SV=1
GG1 pKa = 5.55MTPAFGSLGFRR12 pKa = 11.84GMTPVFGSLGFRR24 pKa = 11.84GLTPAFGSLGFRR36 pKa = 11.84GMTPAFGSLGFRR48 pKa = 11.84GMTPAFGSLGFRR60 pKa = 11.84GMTPAFGSLGFRR72 pKa = 11.84GLTPAFGSLGFRR84 pKa = 11.84GLTPASGSLGFRR96 pKa = 11.84GLTPASGSSGFRR108 pKa = 11.84GLTPASGSSGFRR120 pKa = 11.84GLTPAVGSLGFRR132 pKa = 11.84GLTPAVGSLGFRR144 pKa = 11.84GLTPAFGSLGFRR156 pKa = 11.84GLTPAVGSLGFRR168 pKa = 11.84GLTPASSSLGFRR180 pKa = 11.84GLTPASGSLGGLTPAFGSLGFRR202 pKa = 11.84GLTPAFGSKK211 pKa = 9.57GFRR214 pKa = 11.84GLTPVFGFLGFRR226 pKa = 11.84GLTPAFGSLGFRR238 pKa = 11.84GMTPAFGSLGFRR250 pKa = 11.84GMTPAVGSLGFRR262 pKa = 11.84GLTPAVGSLGIRR274 pKa = 11.84GLTPASGSLGFRR286 pKa = 11.84GLTPAVGSLGFRR298 pKa = 11.84GAAPPPGVYY307 pKa = 9.94RR308 pKa = 11.84MALLLFLPRR317 pKa = 11.84CLKK320 pKa = 10.73SIIRR324 pKa = 11.84LQMAGYY330 pKa = 10.49ARR332 pKa = 4.0

Molecular weight: 33.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38034

28500

66534

27687073

8

6766

416.1

46.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.701 ± 0.009

1.864 ± 0.005

5.279 ± 0.007

6.529 ± 0.015

4.24 ± 0.007

6.479 ± 0.01

2.355 ± 0.004

5.329 ± 0.008

6.237 ± 0.01

9.743 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.004

4.544 ± 0.006

4.92 ± 0.01

3.781 ± 0.008

5.162 ± 0.008

9.048 ± 0.012

4.873 ± 0.006

6.465 ± 0.007

1.247 ± 0.004

2.782 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski