Cyanophage KBS-S-2A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SNT1|M4SNT1_9CAUD Uncharacterized protein OS=Cyanophage KBS-S-2A OX=889953 GN=CPKG_00052 PE=4 SV=1
MM1 pKa = 7.62SDD3 pKa = 3.09EE4 pKa = 4.98PIRR7 pKa = 11.84FIASDD12 pKa = 3.61NQSEE16 pKa = 4.69YY17 pKa = 11.04VSPGTPLPVALTGLEE32 pKa = 4.31IPAHH36 pKa = 6.67DD37 pKa = 5.29YY38 pKa = 11.24IDD40 pKa = 3.97LSYY43 pKa = 10.57TGSNLTSVVYY53 pKa = 10.55KK54 pKa = 10.74DD55 pKa = 3.69GGSGGTTVATLTLAYY70 pKa = 10.23DD71 pKa = 4.0GNDD74 pKa = 3.02NLISVTKK81 pKa = 10.49SS82 pKa = 2.74

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SMT6|M4SMT6_9CAUD Uncharacterized protein OS=Cyanophage KBS-S-2A OX=889953 GN=CPKG_00046 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.47RR3 pKa = 11.84GDD5 pKa = 3.71RR6 pKa = 11.84VSWTYY11 pKa = 9.88QGKK14 pKa = 7.98RR15 pKa = 11.84TYY17 pKa = 11.13GVITSIGGKK26 pKa = 9.36RR27 pKa = 11.84ATINTTSGGTVTRR40 pKa = 11.84VGTDD44 pKa = 2.82DD45 pKa = 3.82DD46 pKa = 4.26QIVRR50 pKa = 11.84IKK52 pKa = 10.81SEE54 pKa = 4.16STGNPVLKK62 pKa = 9.99LQSEE66 pKa = 4.72IKK68 pKa = 9.6PAPKK72 pKa = 9.94RR73 pKa = 11.84RR74 pKa = 3.53

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12716

49

1107

198.7

21.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.082 ± 0.494

0.952 ± 0.148

5.937 ± 0.334

5.646 ± 0.354

3.295 ± 0.15

7.534 ± 0.409

1.486 ± 0.183

5.859 ± 0.2

4.53 ± 0.39

8.163 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.169

4.412 ± 0.205

4.451 ± 0.283

4.852 ± 0.227

5.568 ± 0.358

6.763 ± 0.355

7.793 ± 0.461

6.283 ± 0.314

1.4 ± 0.151

3.295 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski