Ustilaginoidea virens unassigned RNA virus HNND-1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3ZCM4|A0A0G3ZCM4_9VIRU Uncharacterized protein OS=Ustilaginoidea virens unassigned RNA virus HNND-1 OX=1670975 PE=4 SV=1
MM1 pKa = 7.29TCMAGPSEE9 pKa = 4.35EE10 pKa = 4.17VVMSEE15 pKa = 4.29FVPPAISDD23 pKa = 4.07LYY25 pKa = 11.28KK26 pKa = 10.89HH27 pKa = 6.0MEE29 pKa = 4.12GFGNRR34 pKa = 11.84NPGRR38 pKa = 11.84VKK40 pKa = 9.91PTTDD44 pKa = 2.67TYY46 pKa = 11.75AGLFSEE52 pKa = 4.69LRR54 pKa = 11.84AVEE57 pKa = 3.93GWTRR61 pKa = 11.84SISGWLDD68 pKa = 3.06PALLPKK74 pKa = 10.11IVVPSQTSPGIRR86 pKa = 11.84WKK88 pKa = 10.76RR89 pKa = 11.84LGYY92 pKa = 7.65RR93 pKa = 11.84TKK95 pKa = 10.6RR96 pKa = 11.84AALMPAVVEE105 pKa = 4.05ASRR108 pKa = 11.84LVRR111 pKa = 11.84RR112 pKa = 11.84MADD115 pKa = 2.77TGEE118 pKa = 4.23KK119 pKa = 10.75YY120 pKa = 9.59EE121 pKa = 4.06VPPAGVAGRR130 pKa = 11.84GKK132 pKa = 10.54RR133 pKa = 11.84LDD135 pKa = 3.42SCRR138 pKa = 11.84DD139 pKa = 3.1TNAPDD144 pKa = 3.4RR145 pKa = 11.84KK146 pKa = 9.02EE147 pKa = 3.67GRR149 pKa = 11.84LIVMPDD155 pKa = 3.6LVRR158 pKa = 11.84HH159 pKa = 6.15LLGTMAAGPYY169 pKa = 8.77MSMVKK174 pKa = 10.54GLDD177 pKa = 3.36KK178 pKa = 10.95RR179 pKa = 11.84DD180 pKa = 3.48GGVLLGMGPLSDD192 pKa = 3.77SYY194 pKa = 11.64QRR196 pKa = 11.84IADD199 pKa = 3.62WCKK202 pKa = 10.13GADD205 pKa = 3.31SFIFLDD211 pKa = 4.77FSKK214 pKa = 10.62FDD216 pKa = 3.29QRR218 pKa = 11.84LPRR221 pKa = 11.84DD222 pKa = 3.47LLQQVMKK229 pKa = 10.27HH230 pKa = 4.32IRR232 pKa = 11.84GRR234 pKa = 11.84FDD236 pKa = 3.18NVPGGRR242 pKa = 11.84AYY244 pKa = 9.54WEE246 pKa = 4.48SEE248 pKa = 4.02CEE250 pKa = 3.96QLIDD254 pKa = 3.82TQIAMPDD261 pKa = 3.23GNVYY265 pKa = 10.45RR266 pKa = 11.84KK267 pKa = 9.64SQGVASGDD275 pKa = 3.17PWTSIVGSYY284 pKa = 10.96ANWIILRR291 pKa = 11.84RR292 pKa = 11.84VCDD295 pKa = 3.78VLGLRR300 pKa = 11.84AKK302 pKa = 9.93IWTFGDD308 pKa = 3.42DD309 pKa = 4.26SIIAVYY315 pKa = 8.66GQKK318 pKa = 10.07VGSGIIPRR326 pKa = 11.84IAEE329 pKa = 4.0VALSEE334 pKa = 4.82FNMQVSMEE342 pKa = 4.02KK343 pKa = 10.21SYY345 pKa = 11.84ASTTLVDD352 pKa = 4.06IDD354 pKa = 5.42DD355 pKa = 4.71DD356 pKa = 4.42PEE358 pKa = 4.14PHH360 pKa = 6.37VSGSFLSLYY369 pKa = 10.3FLQTPMGVRR378 pKa = 11.84PTRR381 pKa = 11.84PLQDD385 pKa = 3.44LHH387 pKa = 8.56EE388 pKa = 4.63MFLVPEE394 pKa = 4.55RR395 pKa = 11.84NRR397 pKa = 11.84KK398 pKa = 7.88DD399 pKa = 3.56LRR401 pKa = 11.84WEE403 pKa = 3.77IVRR406 pKa = 11.84TSMAYY411 pKa = 10.12LVFYY415 pKa = 10.58YY416 pKa = 10.34NEE418 pKa = 3.76KK419 pKa = 9.75ARR421 pKa = 11.84YY422 pKa = 9.02VIEE425 pKa = 5.23EE426 pKa = 4.04YY427 pKa = 10.14WDD429 pKa = 4.02WIHH432 pKa = 6.24TRR434 pKa = 11.84YY435 pKa = 9.72KK436 pKa = 10.28VPQLRR441 pKa = 11.84GTAEE445 pKa = 4.33DD446 pKa = 3.61LTLLRR451 pKa = 11.84EE452 pKa = 4.02MDD454 pKa = 3.82IPWSAFKK461 pKa = 10.6WEE463 pKa = 3.88WLEE466 pKa = 3.69RR467 pKa = 11.84LPRR470 pKa = 11.84PGEE473 pKa = 3.89VEE475 pKa = 4.29LLYY478 pKa = 10.71KK479 pKa = 10.52YY480 pKa = 10.56GHH482 pKa = 6.58TGFFPPSLWGAWYY495 pKa = 10.48SKK497 pKa = 11.32YY498 pKa = 11.0DD499 pKa = 4.92DD500 pKa = 5.78DD501 pKa = 6.02DD502 pKa = 4.6LGNRR506 pKa = 11.84VSLPMRR512 pKa = 11.84TPQGSDD518 pKa = 2.59HH519 pKa = 6.97GGGPVNN525 pKa = 4.16

Molecular weight: 59.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3ZCM4|A0A0G3ZCM4_9VIRU Uncharacterized protein OS=Ustilaginoidea virens unassigned RNA virus HNND-1 OX=1670975 PE=4 SV=1
MM1 pKa = 7.62SSTHH5 pKa = 5.25TPKK8 pKa = 8.43TTAEE12 pKa = 4.12VKK14 pKa = 9.86PVSSSLPAVGTPAQKK29 pKa = 10.78GAGAKK34 pKa = 9.4GKK36 pKa = 9.78RR37 pKa = 11.84VSGSSTASGGVRR49 pKa = 11.84EE50 pKa = 4.99GFDD53 pKa = 4.25PDD55 pKa = 3.9PEE57 pKa = 4.5SALSVNWASGYY68 pKa = 10.31GVSTPAVTLDD78 pKa = 3.52IIRR81 pKa = 11.84RR82 pKa = 11.84FLMHH86 pKa = 6.32QGVSALPRR94 pKa = 11.84GVEE97 pKa = 4.38DD98 pKa = 5.28LFPSDD103 pKa = 2.77WRR105 pKa = 11.84VGKK108 pKa = 8.52TPVPLEE114 pKa = 3.69WDD116 pKa = 3.93TVGGRR121 pKa = 11.84ALAGVLPNIRR131 pKa = 11.84AYY133 pKa = 10.5DD134 pKa = 3.39VDD136 pKa = 3.14GRR138 pKa = 11.84EE139 pKa = 3.99IHH141 pKa = 5.91WVGEE145 pKa = 4.12GRR147 pKa = 11.84DD148 pKa = 3.36ISHH151 pKa = 7.77ASRR154 pKa = 11.84LKK156 pKa = 10.97AEE158 pKa = 3.86WDD160 pKa = 3.47APITIVAAILTAVKK174 pKa = 9.84KK175 pKa = 10.84AKK177 pKa = 10.11ISEE180 pKa = 4.14EE181 pKa = 4.01DD182 pKa = 3.42VKK184 pKa = 11.19LVAQHH189 pKa = 6.53SPQALEE195 pKa = 3.88KK196 pKa = 10.66LKK198 pKa = 10.78EE199 pKa = 3.8AGAAYY204 pKa = 9.09KK205 pKa = 10.68AHH207 pKa = 7.29LNRR210 pKa = 11.84FLDD213 pKa = 4.18PVALRR218 pKa = 11.84RR219 pKa = 11.84DD220 pKa = 3.51PRR222 pKa = 11.84YY223 pKa = 10.18GEE225 pKa = 3.59VAARR229 pKa = 11.84YY230 pKa = 8.51KK231 pKa = 11.07AEE233 pKa = 3.76IRR235 pKa = 11.84SLVAEE240 pKa = 4.12QQAKK244 pKa = 9.33IVEE247 pKa = 4.18ARR249 pKa = 11.84KK250 pKa = 8.12VTRR253 pKa = 11.84RR254 pKa = 11.84LNQLLGQRR262 pKa = 11.84DD263 pKa = 3.74EE264 pKa = 4.77EE265 pKa = 4.41LAKK268 pKa = 10.35LDD270 pKa = 4.09PGFTPKK276 pKa = 10.45RR277 pKa = 11.84ATAAAEE283 pKa = 3.83LAKK286 pKa = 10.72FGITLEE292 pKa = 4.21EE293 pKa = 4.51GEE295 pKa = 4.32EE296 pKa = 4.21AEE298 pKa = 4.31SAEE301 pKa = 4.44AGLTAQMLEE310 pKa = 4.33DD311 pKa = 4.29LNFF314 pKa = 3.84

Molecular weight: 33.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

839

314

525

419.5

46.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.82 ± 2.828

0.596 ± 0.346

6.317 ± 0.709

6.555 ± 1.001

2.861 ± 0.366

8.582 ± 0.175

1.669 ± 0.141

4.291 ± 0.272

5.125 ± 0.722

8.939 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 1.036

1.907 ± 0.183

6.436 ± 0.408

2.741 ± 0.072

7.747 ± 0.43

6.675 ± 0.008

4.768 ± 0.375

7.509 ± 0.263

2.503 ± 0.528

3.218 ± 0.943
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski