Novipirellula galeiformis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Novipirellula

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6CBW5|A0A5C6CBW5_9BACT Uncharacterized protein OS=Novipirellula galeiformis OX=2528004 GN=Pla52o_37120 PE=4 SV=1
MM1 pKa = 7.62GSSQYY6 pKa = 11.27SILTDD11 pKa = 3.62EE12 pKa = 4.61SWISDD17 pKa = 3.53VPSAEE22 pKa = 3.75KK23 pKa = 10.15TRR25 pKa = 11.84FDD27 pKa = 4.25MIIPYY32 pKa = 9.41WGTNTDD38 pKa = 3.8DD39 pKa = 4.33AGCVYY44 pKa = 10.56QGISDD49 pKa = 3.92GRR51 pKa = 11.84NASIQAFYY59 pKa = 10.23QASNIDD65 pKa = 3.3EE66 pKa = 4.54RR67 pKa = 11.84YY68 pKa = 10.15EE69 pKa = 3.84NN70 pKa = 3.79

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6CM81|A0A5C6CM81_9BACT 31-O-demethyl-FK506 methyltransferase FkbM OS=Novipirellula galeiformis OX=2528004 GN=fkbM PE=4 SV=1
MM1 pKa = 7.16IHH3 pKa = 7.09RR4 pKa = 11.84LRR6 pKa = 11.84RR7 pKa = 11.84LRR9 pKa = 11.84RR10 pKa = 11.84VSALRR15 pKa = 11.84FALGGALHH23 pKa = 6.61LTPNTPNRR31 pKa = 11.84MRR33 pKa = 11.84PNHH36 pKa = 5.13VPAWSMVV43 pKa = 2.99

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5747

0

5747

2082698

29

8578

362.4

39.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.942 ± 0.033

1.141 ± 0.016

6.093 ± 0.033

5.939 ± 0.036

3.65 ± 0.022

7.587 ± 0.05

2.314 ± 0.022

5.2 ± 0.018

3.497 ± 0.036

9.523 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.022

3.285 ± 0.039

5.158 ± 0.031

4.055 ± 0.025

6.63 ± 0.047

6.885 ± 0.035

5.772 ± 0.07

7.232 ± 0.031

1.441 ± 0.015

2.331 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski