Aeromonas phage AhyVDH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6H1NP77|A0A6H1NP77_9CAUD Uncharacterized protein OS=Aeromonas phage AhyVDH1 OX=2724309 GN=AhyVDH1_056 PE=4 SV=1
MM1 pKa = 7.34NMKK4 pKa = 10.24NLDD7 pKa = 3.72HH8 pKa = 5.74QQCSILLGFAEE19 pKa = 4.65EE20 pKa = 3.93NWEE23 pKa = 3.97QFVAHH28 pKa = 5.75VAGHH32 pKa = 5.93NGITEE37 pKa = 4.11EE38 pKa = 3.98EE39 pKa = 4.37AEE41 pKa = 4.63AEE43 pKa = 4.08AEE45 pKa = 4.57DD46 pKa = 3.8IVEE49 pKa = 4.27ALGEE53 pKa = 4.25GANGG57 pKa = 3.39

Molecular weight:
6.2 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6H1NP78|A0A6H1NP78_9CAUD Baseplate assembly protein V OS=Aeromonas phage AhyVDH1 OX=2724309 GN=AhyVDH1_020 PE=4 SV=1
MM1 pKa = 7.19TRR3 pKa = 11.84RR4 pKa = 11.84PVPIVATSIQTGEE17 pKa = 4.06VFNYY21 pKa = 8.95PSRR24 pKa = 11.84AAAAEE29 pKa = 4.1SGGFCAATITLCIRR43 pKa = 11.84GIVPSHH49 pKa = 6.69AGFKK53 pKa = 9.71FHH55 pKa = 7.1ATAKK59 pKa = 9.76LDD61 pKa = 3.54PPRR64 pKa = 11.84RR65 pKa = 11.84PKK67 pKa = 10.35LAAKK71 pKa = 9.97VKK73 pKa = 8.67EE74 pKa = 3.99LRR76 pKa = 11.84ATGLSYY82 pKa = 11.49AKK84 pKa = 9.15TAKK87 pKa = 10.6ALGISEE93 pKa = 4.09STAYY97 pKa = 10.11YY98 pKa = 9.4HH99 pKa = 6.86ARR101 pKa = 11.84NRR103 pKa = 11.84AA104 pKa = 3.05

Molecular weight:
11.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11512

44

869

195.1

21.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.954 ± 0.616

1.173 ± 0.127

5.299 ± 0.283

6.246 ± 0.283

2.936 ± 0.17

8.0 ± 0.376

2.163 ± 0.209

4.917 ± 0.194

5.837 ± 0.266

8.383 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.162 ± 0.166

3.37 ± 0.243

4.421 ± 0.299

3.952 ± 0.231

6.055 ± 0.335

5.881 ± 0.235

6.194 ± 0.263

7.149 ± 0.308

1.581 ± 0.136

2.328 ± 0.175

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski