Erwinia phage Ea9-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Erskinevirinae; Johnsonvirus; Erwinia virus Ea9-2

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6ASR7|W6ASR7_9CAUD Virion-associated RNA polymerase OS=Erwinia phage Ea9-2 OX=1429767 GN=Ea92_69 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 3.71KK4 pKa = 10.85PDD6 pKa = 4.56LISNNLLFPAGTRR19 pKa = 11.84LMCPNCGTISNNHH32 pKa = 6.43KK33 pKa = 10.69ASLPLYY39 pKa = 7.48VQYY42 pKa = 11.13RR43 pKa = 11.84HH44 pKa = 7.13GIPDD48 pKa = 3.39NPQWVSFHH56 pKa = 6.35TCPCGYY62 pKa = 10.08CDD64 pKa = 4.82DD65 pKa = 4.5SGGGFTDD72 pKa = 3.34SGNYY76 pKa = 9.61EE77 pKa = 4.16CDD79 pKa = 3.51DD80 pKa = 3.63TRR82 pKa = 11.84DD83 pKa = 3.38IQKK86 pKa = 9.8WEE88 pKa = 4.05EE89 pKa = 3.7EE90 pKa = 3.98LAEE93 pKa = 4.35LEE95 pKa = 4.44EE96 pKa = 4.27EE97 pKa = 4.22MEE99 pKa = 4.0EE100 pKa = 4.65LKK102 pKa = 11.34YY103 pKa = 10.66KK104 pKa = 9.07IQKK107 pKa = 10.29GKK109 pKa = 10.42DD110 pKa = 2.72RR111 pKa = 11.84WEE113 pKa = 4.2EE114 pKa = 3.51LWGTNEE120 pKa = 3.85DD121 pKa = 2.93RR122 pKa = 11.84CYY124 pKa = 11.33

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6ASQ0|W6ASQ0_9CAUD Polynucleotide kinase OS=Erwinia phage Ea9-2 OX=1429767 GN=Ea92_61 PE=4 SV=1
MM1 pKa = 6.88MRR3 pKa = 11.84RR4 pKa = 11.84VPFLGSRR11 pKa = 11.84SFTSSNSFSRR21 pKa = 11.84RR22 pKa = 11.84VGTVLPVFTTQPVTQSLFQEE42 pKa = 4.42NGPLLITGAVVRR54 pKa = 11.84NASSYY59 pKa = 7.46QWQRR63 pKa = 11.84STDD66 pKa = 3.28GVTFTDD72 pKa = 4.02VAGQTAAVLNQTGVTGGSATYY93 pKa = 10.1RR94 pKa = 11.84LKK96 pKa = 10.95AINDD100 pKa = 3.49KK101 pKa = 10.89GFKK104 pKa = 9.97YY105 pKa = 10.72SNNVQVLSAYY115 pKa = 10.73LSIQNDD121 pKa = 3.33SSSADD126 pKa = 3.26GSALGTTKK134 pKa = 9.21VTNSTYY140 pKa = 8.67THH142 pKa = 6.71NAPAGNRR149 pKa = 11.84PFSAFYY155 pKa = 10.82RR156 pKa = 11.84LLSDD160 pKa = 3.23NSIFIPEE167 pKa = 4.23GTTSGRR173 pKa = 11.84AVPNWVSSNTAVLPQTPVTATGQLNCATKK202 pKa = 10.53AGSATLTVSIGRR214 pKa = 11.84LSSVLSATITT224 pKa = 3.32

Molecular weight: 23.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

23728

42

3523

252.4

27.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.196 ± 0.459

0.877 ± 0.166

6.094 ± 0.126

6.204 ± 0.324

3.477 ± 0.177

6.638 ± 0.223

1.955 ± 0.18

5.42 ± 0.226

5.976 ± 0.284

8.332 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.051 ± 0.15

5.432 ± 0.24

4.147 ± 0.183

4.446 ± 0.361

4.484 ± 0.166

6.077 ± 0.214

6.566 ± 0.244

7.236 ± 0.259

1.129 ± 0.116

3.262 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski