Microterricola viridarii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microterricola

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Y0Q264|A0A0Y0Q264_9MICO HTH tetR-type domain-containing protein OS=Microterricola viridarii OX=412690 GN=AWU67_16415 PE=4 SV=1
MM1 pKa = 8.0PLTMLPTTRR10 pKa = 11.84GAARR14 pKa = 11.84VSLAALLAVGVLVGASGCTAEE35 pKa = 4.77QPASGEE41 pKa = 4.16KK42 pKa = 10.78GSWTVLTYY50 pKa = 10.76AIADD54 pKa = 3.93TDD56 pKa = 3.99LEE58 pKa = 4.58PYY60 pKa = 10.06MMEE63 pKa = 5.03DD64 pKa = 3.41LDD66 pKa = 3.99EE67 pKa = 5.66LGEE70 pKa = 4.27VGSSDD75 pKa = 3.91ALNLVALVDD84 pKa = 3.75RR85 pKa = 11.84AEE87 pKa = 5.05DD88 pKa = 3.75YY89 pKa = 11.73GDD91 pKa = 4.3DD92 pKa = 3.98PVLGMEE98 pKa = 4.76GWSGARR104 pKa = 11.84LLEE107 pKa = 4.04VGKK110 pKa = 10.76RR111 pKa = 11.84SATVLEE117 pKa = 4.18EE118 pKa = 4.05MGDD121 pKa = 3.84VNTGDD126 pKa = 3.69PAVLAEE132 pKa = 4.46FIEE135 pKa = 4.61RR136 pKa = 11.84GIAEE140 pKa = 4.37YY141 pKa = 9.15PADD144 pKa = 4.18NYY146 pKa = 11.78ALLISDD152 pKa = 5.39HH153 pKa = 5.99GASWPGVGGDD163 pKa = 3.78EE164 pKa = 4.66SAHH167 pKa = 6.57EE168 pKa = 4.27DD169 pKa = 3.67TLTLAEE175 pKa = 4.72LDD177 pKa = 3.67QAIGDD182 pKa = 4.11GLSAAGVDD190 pKa = 4.07SLDD193 pKa = 3.65LLGFDD198 pKa = 4.12ACLMATYY205 pKa = 9.83EE206 pKa = 4.35VASALAPHH214 pKa = 6.99AQRR217 pKa = 11.84LLASQEE223 pKa = 4.13LEE225 pKa = 4.43PGHH228 pKa = 6.05GWDD231 pKa = 3.72YY232 pKa = 10.15TALGILTDD240 pKa = 3.95GGVSVDD246 pKa = 3.76EE247 pKa = 4.9LGVAIIDD254 pKa = 3.86GFEE257 pKa = 3.99AQAQAEE263 pKa = 4.46GTEE266 pKa = 4.3TEE268 pKa = 4.09ITLSLVDD275 pKa = 4.08LTQMPKK281 pKa = 9.96VDD283 pKa = 3.42AALTNFTSVLTARR296 pKa = 11.84AAIAGPVVGRR306 pKa = 11.84TLAKK310 pKa = 9.11TLGFGRR316 pKa = 11.84SPDD319 pKa = 3.66PTEE322 pKa = 4.57DD323 pKa = 2.84SHH325 pKa = 6.99MADD328 pKa = 3.67LGILAGEE335 pKa = 4.25IGVDD339 pKa = 3.17ALYY342 pKa = 10.95ASDD345 pKa = 4.59AADD348 pKa = 3.7DD349 pKa = 4.02LVRR352 pKa = 11.84AINDD356 pKa = 3.32AVVYY360 pKa = 10.4KK361 pKa = 10.73VDD363 pKa = 3.58GQATRR368 pKa = 11.84GATGLSIYY376 pKa = 9.86FPPTPEE382 pKa = 4.46LFLDD386 pKa = 4.5EE387 pKa = 4.25YY388 pKa = 11.33RR389 pKa = 11.84EE390 pKa = 4.12LGNTGGWADD399 pKa = 4.09FLLAFYY405 pKa = 10.53GAGTDD410 pKa = 3.89LPVDD414 pKa = 3.26QAVRR418 pKa = 11.84FNGGAAGTLFDD429 pKa = 4.89EE430 pKa = 6.09DD431 pKa = 4.41GLTIQGTVDD440 pKa = 3.09AALIDD445 pKa = 5.28AIADD449 pKa = 3.38SYY451 pKa = 10.95IRR453 pKa = 11.84YY454 pKa = 9.83GIVEE458 pKa = 4.61DD459 pKa = 4.99DD460 pKa = 4.27GSVSYY465 pKa = 10.56IGQEE469 pKa = 3.72TATALGGDD477 pKa = 3.82SGVVQGTYY485 pKa = 11.08DD486 pKa = 3.4LTRR489 pKa = 11.84FMISDD494 pKa = 4.11GEE496 pKa = 4.34DD497 pKa = 3.06SVPAYY502 pKa = 10.31LQLAEE507 pKa = 4.65EE508 pKa = 4.41SDD510 pKa = 4.1GVISISAPMGYY521 pKa = 9.6YY522 pKa = 10.3APHH525 pKa = 7.49DD526 pKa = 3.97IDD528 pKa = 5.32GEE530 pKa = 4.76TYY532 pKa = 10.48TDD534 pKa = 3.7ALLSLTVDD542 pKa = 3.42ADD544 pKa = 3.81SGDD547 pKa = 3.99VLSEE551 pKa = 3.94TYY553 pKa = 10.97YY554 pKa = 10.93SFKK557 pKa = 10.21PGPGAYY563 pKa = 9.42GQLSVKK569 pKa = 8.74PTGLIVPEE577 pKa = 4.06VLSVLEE583 pKa = 5.34DD584 pKa = 3.67GTEE587 pKa = 3.75QWVAPTGRR595 pKa = 11.84GVYY598 pKa = 10.22AKK600 pKa = 10.48LADD603 pKa = 3.96LGYY606 pKa = 10.88DD607 pKa = 3.92FVPLASTTKK616 pKa = 10.55LVIEE620 pKa = 4.33LWVVDD625 pKa = 4.1FAGNSDD631 pKa = 3.58MVSAQVEE638 pKa = 4.77VPP640 pKa = 3.34

Molecular weight: 66.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A120I1A9|A0A120I1A9_9MICO Uncharacterized protein OS=Microterricola viridarii OX=412690 GN=AWU67_15615 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILGARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.21GRR40 pKa = 11.84VEE42 pKa = 4.17LSAA45 pKa = 5.19

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3119

0

3119

1022284

38

3052

327.8

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.975 ± 0.062

0.496 ± 0.011

5.59 ± 0.034

5.565 ± 0.045

3.277 ± 0.026

9.097 ± 0.042

1.918 ± 0.02

4.739 ± 0.033

2.117 ± 0.034

10.523 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.868 ± 0.019

2.265 ± 0.025

5.26 ± 0.033

2.973 ± 0.022

6.463 ± 0.044

5.916 ± 0.031

5.953 ± 0.047

8.61 ± 0.041

1.437 ± 0.02

1.961 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski