Flavobacterium sp. AG291

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370EB97|A0A370EB97_9FLAO Alanine dehydrogenase OS=Flavobacterium sp. AG291 OX=2184000 GN=DEU42_12017 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.46RR3 pKa = 11.84LLPVLLILLGVYY15 pKa = 7.59TANAQVGIGTTTPNPSAQLQIVSGDD40 pKa = 3.38KK41 pKa = 10.88GVLIPQVQLTSTTDD55 pKa = 2.86TTTITNGNVQSLLVYY70 pKa = 10.68NITSNSQIKK79 pKa = 8.82PGYY82 pKa = 8.67YY83 pKa = 8.62YY84 pKa = 9.93WYY86 pKa = 7.96VNKK89 pKa = 9.74WYY91 pKa = 10.56RR92 pKa = 11.84LLSDD96 pKa = 4.02GDD98 pKa = 3.8AGQGDD103 pKa = 4.49SAQPIIDD110 pKa = 4.43NGNGTYY116 pKa = 9.96TYY118 pKa = 8.93TNPDD122 pKa = 3.06GSVTTIDD129 pKa = 3.45VPGNQTLTFLVYY141 pKa = 10.88NPTAKK146 pKa = 9.89TLTYY150 pKa = 9.41TAEE153 pKa = 3.96NGFNTVIDD161 pKa = 4.24LDD163 pKa = 4.23AVVKK167 pKa = 10.79ASEE170 pKa = 4.37TVTTLVDD177 pKa = 3.48NGNGTVTFTNEE188 pKa = 3.45AGVATTINLAGGTQGVGIASTVDD211 pKa = 3.11NGDD214 pKa = 3.19GTFTINYY221 pKa = 7.68TDD223 pKa = 3.52GTSFTTSNLTGPQGPQGQAGPAAAPGVGIASTVDD257 pKa = 3.11NGDD260 pKa = 3.19GTFTINYY267 pKa = 7.15TNGTSFTTTSLLGPQGPVGATGAQGVAGATGADD300 pKa = 3.4GKK302 pKa = 11.16GITSTTDD309 pKa = 2.77NGNGTFTINYY319 pKa = 7.65TDD321 pKa = 3.52GTSFTTSNLTGATGAQGATGADD343 pKa = 3.15GKK345 pKa = 11.19GIVSTTDD352 pKa = 2.83NGNGTFTINFTDD364 pKa = 3.97GTSFTTSNLTGPQGATGTQGIQGPQGATGNDD395 pKa = 3.83GVAGPQGATGADD407 pKa = 3.26GKK409 pKa = 11.34GIATTVDD416 pKa = 3.09NGNGTFTINYY426 pKa = 7.65TDD428 pKa = 3.52GTSFTTSNLTGPQGATGTQGIQGPQGATGNDD459 pKa = 3.83GVAGPQGATGADD471 pKa = 3.29GKK473 pKa = 11.14GIASTTDD480 pKa = 2.91NGNGTFTINYY490 pKa = 7.65TDD492 pKa = 3.52GTSFTTSNLTGPQGATGTQGIQGPQGATGNDD523 pKa = 3.83GVAGPQGATGADD535 pKa = 3.29GKK537 pKa = 11.19GIASTVDD544 pKa = 3.01NGNGTFTINYY554 pKa = 7.65TDD556 pKa = 3.52GTSFTTSNLTGPQGATGTQGIQGPQGATGNDD587 pKa = 3.83GVAGPQGATGADD599 pKa = 3.29GKK601 pKa = 11.14GIASTTDD608 pKa = 2.91NGNGTFTT615 pKa = 4.11

Molecular weight: 60.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370EM15|A0A370EM15_9FLAO Uncharacterized protein OS=Flavobacterium sp. AG291 OX=2184000 GN=DEU42_10668 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.53HH17 pKa = 4.24GFMEE21 pKa = 4.81RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3455

0

3455

1150073

26

5333

332.9

37.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.17 ± 0.054

0.816 ± 0.018

5.501 ± 0.034

6.238 ± 0.053

4.981 ± 0.041

6.724 ± 0.047

1.694 ± 0.023

7.441 ± 0.05

7.243 ± 0.078

8.809 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.025

6.06 ± 0.039

3.647 ± 0.033

3.336 ± 0.024

3.32 ± 0.035

6.309 ± 0.04

6.549 ± 0.106

6.524 ± 0.043

1.053 ± 0.016

4.265 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski