Microviridae sp. ctQch27

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 9.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8W3|A0A5Q2W8W3_9VIRU Major capsid protein OS=Microviridae sp. ctQch27 OX=2656699 PE=3 SV=1
MM1 pKa = 7.08KK2 pKa = 10.24RR3 pKa = 11.84SVFSLSHH10 pKa = 6.36HH11 pKa = 6.51KK12 pKa = 10.53LLSFDD17 pKa = 3.38MGEE20 pKa = 4.12LVPIGMTEE28 pKa = 4.11VLPGDD33 pKa = 4.0TMQQATTMLVRR44 pKa = 11.84ASPLLAPVMHH54 pKa = 6.74PVDD57 pKa = 3.67VRR59 pKa = 11.84IHH61 pKa = 5.6HH62 pKa = 6.51WFVPHH67 pKa = 6.45RR68 pKa = 11.84LVWADD73 pKa = 3.33FEE75 pKa = 4.65KK76 pKa = 10.68FITGGPDD83 pKa = 3.06GLDD86 pKa = 2.67ASVFPTITFGGAIPAGTVMDD106 pKa = 4.4YY107 pKa = 11.41LGMPLGVAGLTVSALPFRR125 pKa = 11.84AYY127 pKa = 11.13NLIWNNWYY135 pKa = 10.0RR136 pKa = 11.84DD137 pKa = 3.45QDD139 pKa = 4.16LEE141 pKa = 4.17TALAVSTASGNDD153 pKa = 2.95VTTNTNLKK161 pKa = 8.83SCSWEE166 pKa = 3.51KK167 pKa = 11.19DD168 pKa = 3.38YY169 pKa = 9.31FTSARR174 pKa = 11.84PWEE177 pKa = 4.43QKK179 pKa = 10.73GPTVTVPLGTTAPVMGLGFANTPSQMAASTVMRR212 pKa = 11.84QTTPVANEE220 pKa = 4.22TVNNPPWWMITNAANTMGVKK240 pKa = 10.03ADD242 pKa = 3.7SAASGTTKK250 pKa = 10.39PSVFADD256 pKa = 3.94LTNASAVTVNTLRR269 pKa = 11.84QALAFQRR276 pKa = 11.84YY277 pKa = 7.43EE278 pKa = 3.67EE279 pKa = 3.99ARR281 pKa = 11.84ARR283 pKa = 11.84FGSRR287 pKa = 11.84YY288 pKa = 9.26IEE290 pKa = 3.9YY291 pKa = 10.5LRR293 pKa = 11.84YY294 pKa = 10.12LGVKK298 pKa = 10.15SSDD301 pKa = 3.04GRR303 pKa = 11.84LQVPEE308 pKa = 4.01YY309 pKa = 10.71LGGGRR314 pKa = 11.84DD315 pKa = 3.59TIQFSEE321 pKa = 4.24ILQTAADD328 pKa = 3.88GTNPVGTMRR337 pKa = 11.84GHH339 pKa = 7.34GIAGMRR345 pKa = 11.84SNRR348 pKa = 11.84YY349 pKa = 8.12RR350 pKa = 11.84RR351 pKa = 11.84FFEE354 pKa = 3.53EE355 pKa = 3.29HH356 pKa = 6.73GYY358 pKa = 10.71VMSLASVRR366 pKa = 11.84PKK368 pKa = 9.96TMYY371 pKa = 9.4MQGLARR377 pKa = 11.84TWNRR381 pKa = 11.84RR382 pKa = 11.84VKK384 pKa = 10.55EE385 pKa = 4.12DD386 pKa = 3.37FWQKK390 pKa = 9.59EE391 pKa = 4.02LQFIGQQAIKK401 pKa = 10.73NKK403 pKa = 9.39EE404 pKa = 3.82LYY406 pKa = 9.63AAHH409 pKa = 7.36ASPDD413 pKa = 3.51GTFGYY418 pKa = 9.77QDD420 pKa = 3.56RR421 pKa = 11.84YY422 pKa = 10.84DD423 pKa = 3.41EE424 pKa = 4.2YY425 pKa = 11.03RR426 pKa = 11.84RR427 pKa = 11.84SEE429 pKa = 4.09SSVSGEE435 pKa = 3.49FRR437 pKa = 11.84TVLNFWHH444 pKa = 7.24FARR447 pKa = 11.84EE448 pKa = 4.28FGSSPALNGTFVSCVPTEE466 pKa = 3.8RR467 pKa = 11.84TFAVSTNDD475 pKa = 3.5VLWVMAKK482 pKa = 10.28HH483 pKa = 6.63SIQARR488 pKa = 11.84RR489 pKa = 11.84LVAKK493 pKa = 9.13TGRR496 pKa = 11.84SMTFF500 pKa = 2.75

Molecular weight: 55.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W671|A0A5Q2W671_9VIRU Putative minor capsid protein OS=Microviridae sp. ctQch27 OX=2656699 PE=4 SV=1
MM1 pKa = 7.18SLKK4 pKa = 10.38RR5 pKa = 11.84LRR7 pKa = 11.84KK8 pKa = 8.93RR9 pKa = 11.84RR10 pKa = 11.84ISRR13 pKa = 11.84LVRR16 pKa = 11.84TRR18 pKa = 11.84VWRR21 pKa = 11.84IGSLTSILLRR31 pKa = 11.84LRR33 pKa = 11.84STFAGRR39 pKa = 11.84KK40 pKa = 7.14PASLCMRR47 pKa = 11.84KK48 pKa = 9.66SSLMKK53 pKa = 10.01KK54 pKa = 10.12VSWFLLKK61 pKa = 10.86GILKK65 pKa = 9.68AFPRR69 pKa = 11.84SRR71 pKa = 11.84PRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84LLDD78 pKa = 3.63GPRR81 pKa = 11.84SPSRR85 pKa = 11.84AAVGRR90 pKa = 11.84SSRR93 pKa = 11.84PLTGFRR99 pKa = 11.84ARR101 pKa = 11.84LFLRR105 pKa = 11.84LLGGVVGNAVHH116 pKa = 7.05SPGEE120 pKa = 3.96LKK122 pKa = 10.81ARR124 pKa = 3.95

Molecular weight: 14.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1303

107

500

260.6

29.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.44 ± 1.1

1.074 ± 0.538

3.147 ± 0.536

4.988 ± 0.854

4.068 ± 0.601

8.289 ± 0.609

1.765 ± 0.301

2.993 ± 0.269

4.451 ± 0.62

8.365 ± 0.934

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.3 ± 0.31

2.916 ± 0.791

5.602 ± 0.655

3.761 ± 0.707

10.207 ± 2.234

7.368 ± 0.57

5.526 ± 1.542

7.368 ± 1.085

2.686 ± 0.45

2.686 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski