Ralstonia phage Heva

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Cimandefvirus; Ralstonia virus Heva

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5BAV8|A0A7G5BAV8_9CAUD Peptidase S74 domain-containing protein OS=Ralstonia phage Heva OX=2759730 GN=F1_00068 PE=4 SV=1
MM1 pKa = 7.53LGIVQKK7 pKa = 9.44TATEE11 pKa = 3.89QLDD14 pKa = 3.56YY15 pKa = 11.26DD16 pKa = 3.64IDD18 pKa = 3.85FARR21 pKa = 11.84WMPDD25 pKa = 2.52GDD27 pKa = 4.01VLQGAGVAITPDD39 pKa = 4.4DD40 pKa = 3.84GTLTSPAYY48 pKa = 9.76EE49 pKa = 3.73IDD51 pKa = 3.6GTVVKK56 pKa = 10.55VWLAGGTAGASYY68 pKa = 11.0NVDD71 pKa = 3.27VTVATAAGRR80 pKa = 11.84IKK82 pKa = 9.03EE83 pKa = 4.4TCFKK87 pKa = 10.58IRR89 pKa = 11.84VRR91 pKa = 11.84SCC93 pKa = 2.82

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5BAR1|A0A7G5BAR1_9CAUD Uncharacterized protein OS=Ralstonia phage Heva OX=2759730 GN=F1_00020 PE=4 SV=1
MM1 pKa = 7.6PCSEE5 pKa = 3.6QRR7 pKa = 11.84ARR9 pKa = 11.84KK10 pKa = 9.03LLSAGRR16 pKa = 11.84ARR18 pKa = 11.84VHH20 pKa = 6.32RR21 pKa = 11.84LYY23 pKa = 10.32PFTIRR28 pKa = 11.84LIDD31 pKa = 3.61RR32 pKa = 11.84QQANSVLQPLEE43 pKa = 4.54LKK45 pKa = 10.42LDD47 pKa = 4.35PGSKK51 pKa = 6.87TTGLAVCRR59 pKa = 11.84VEE61 pKa = 4.85EE62 pKa = 4.68RR63 pKa = 11.84IDD65 pKa = 3.29VDD67 pKa = 3.97GVVEE71 pKa = 4.1RR72 pKa = 11.84VMHH75 pKa = 6.39IRR77 pKa = 11.84FLMDD81 pKa = 3.92LVHH84 pKa = 6.97RR85 pKa = 11.84GHH87 pKa = 7.53AIKK90 pKa = 10.34EE91 pKa = 4.13ALHH94 pKa = 6.32ARR96 pKa = 11.84AAMRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84GNLRR108 pKa = 11.84YY109 pKa = 9.46RR110 pKa = 11.84APRR113 pKa = 11.84FDD115 pKa = 2.98NRR117 pKa = 11.84TRR119 pKa = 11.84KK120 pKa = 9.91EE121 pKa = 3.62GWLPPSLQHH130 pKa = 7.23RR131 pKa = 11.84VDD133 pKa = 3.81TTVSWVKK140 pKa = 10.15RR141 pKa = 11.84LCRR144 pKa = 11.84LAPITHH150 pKa = 7.53LAQEE154 pKa = 4.45LVRR157 pKa = 11.84FDD159 pKa = 3.56MQLMQNAEE167 pKa = 3.87ISGVEE172 pKa = 4.02YY173 pKa = 10.62QQGEE177 pKa = 4.04LAGYY181 pKa = 7.23EE182 pKa = 3.88VRR184 pKa = 11.84EE185 pKa = 3.92YY186 pKa = 11.21LLEE189 pKa = 4.35KK190 pKa = 10.41FGRR193 pKa = 11.84TCQYY197 pKa = 10.63CDD199 pKa = 3.25EE200 pKa = 4.8TGVPLQVEE208 pKa = 4.92HH209 pKa = 6.68IHH211 pKa = 6.08PKK213 pKa = 10.4ARR215 pKa = 11.84GGSNRR220 pKa = 11.84VSNLTLACQPCNEE233 pKa = 4.9KK234 pKa = 10.41KK235 pKa = 10.31DD236 pKa = 3.8SQDD239 pKa = 2.61IRR241 pKa = 11.84VFLAKK246 pKa = 10.57DD247 pKa = 3.18PMRR250 pKa = 11.84LEE252 pKa = 5.13RR253 pKa = 11.84ILARR257 pKa = 11.84AKK259 pKa = 10.84APLRR263 pKa = 11.84DD264 pKa = 3.54AAAVNATRR272 pKa = 11.84WALLNALKK280 pKa = 9.42GTGLPVTTGSGGQTKK295 pKa = 9.43WNRR298 pKa = 11.84TRR300 pKa = 11.84LGLPKK305 pKa = 9.65THH307 pKa = 6.85SLDD310 pKa = 3.5AACVGRR316 pKa = 11.84ADD318 pKa = 3.88RR319 pKa = 11.84VTGSGAPVLVVKK331 pKa = 9.21CTGRR335 pKa = 11.84GSRR338 pKa = 11.84SRR340 pKa = 11.84TRR342 pKa = 11.84LNQYY346 pKa = 8.61GFPRR350 pKa = 11.84AYY352 pKa = 10.63LARR355 pKa = 11.84NKK357 pKa = 9.22TAFGFRR363 pKa = 11.84TGDD366 pKa = 3.32MVIASVPSGKK376 pKa = 10.13KK377 pKa = 9.39AGTYY381 pKa = 9.16KK382 pKa = 10.63GRR384 pKa = 11.84VAIRR388 pKa = 11.84QTGSFNIQPGKK399 pKa = 9.8PGLPTVQGISHH410 pKa = 6.39KK411 pKa = 9.73HH412 pKa = 5.4CSVAQRR418 pKa = 11.84GDD420 pKa = 3.17GYY422 pKa = 11.29GYY424 pKa = 11.51ALASPVNSQPGKK436 pKa = 8.23ATRR439 pKa = 11.84PAASALYY446 pKa = 10.63LLGLKK451 pKa = 10.52AEE453 pKa = 4.39VSRR456 pKa = 11.84ADD458 pKa = 3.14

Molecular weight: 50.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

18022

31

4545

265.0

28.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.212 ± 0.496

0.816 ± 0.234

6.131 ± 0.237

6.32 ± 0.477

2.963 ± 0.135

8.628 ± 0.346

1.948 ± 0.226

4.35 ± 0.232

4.9 ± 0.386

6.964 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.124

3.523 ± 0.242

4.683 ± 0.257

4.278 ± 0.239

7.441 ± 0.386

5.521 ± 0.296

5.205 ± 0.26

6.642 ± 0.292

1.31 ± 0.166

2.552 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski