Amino acid dipepetide frequency for Oikopleura dioica (Tunicate)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.513AlaAla: 5.513 ± 0.052	1.18AlaCys: 1.18 ± 0.019	3.641AlaAsp: 3.641 ± 0.029	4.893AlaGlu: 4.893 ± 0.036	2.703AlaPhe: 2.703 ± 0.023	3.806AlaGly: 3.806 ± 0.041	1.252AlaHis: 1.252 ± 0.014	3.687AlaIle: 3.687 ± 0.027	4.562AlaLys: 4.562 ± 0.034	5.571AlaLeu: 5.571 ± 0.039	1.434AlaMet: 1.434 ± 0.015	2.839AlaAsn: 2.839 ± 0.02	2.933AlaPro: 2.933 ± 0.03	2.707AlaGln: 2.707 ± 0.023	3.426AlaArg: 3.426 ± 0.025	4.849AlaSer: 4.849 ± 0.029	3.477AlaThr: 3.477 ± 0.024	3.727AlaVal: 3.727 ± 0.025	0.658AlaTrp: 0.658 ± 0.01	1.593AlaTyr: 1.593 ± 0.019	0.002AlaXaa: 0.002 ± 0.001
Cys	1.32CysAla: 1.32 ± 0.02	0.536CysCys: 0.536 ± 0.016	1.373CysAsp: 1.373 ± 0.026	1.519CysGlu: 1.519 ± 0.029	0.996CysPhe: 0.996 ± 0.013	1.405CysGly: 1.405 ± 0.023	0.489CysHis: 0.489 ± 0.01	1.052CysIle: 1.052 ± 0.015	1.232CysLys: 1.232 ± 0.02	1.698CysLeu: 1.698 ± 0.02	0.411CysMet: 0.411 ± 0.009	0.927CysAsn: 0.927 ± 0.017	1.278CysPro: 1.278 ± 0.061	0.836CysGln: 0.836 ± 0.014	1.055CysArg: 1.055 ± 0.014	1.74CysSer: 1.74 ± 0.026	1.062CysThr: 1.062 ± 0.019	1.195CysVal: 1.195 ± 0.02	0.277CysTrp: 0.277 ± 0.007	0.598CysTyr: 0.598 ± 0.011	0.001CysXaa: 0.001 ± 0.0
Asp	3.512AspAla: 3.512 ± 0.028	1.271AspCys: 1.271 ± 0.023	4.154AspAsp: 4.154 ± 0.033	5.286AspGlu: 5.286 ± 0.037	3.068AspPhe: 3.068 ± 0.024	3.669AspGly: 3.669 ± 0.032	1.141AspHis: 1.141 ± 0.013	3.295AspIle: 3.295 ± 0.027	3.327AspLys: 3.327 ± 0.024	5.061AspLeu: 5.061 ± 0.033	1.173AspMet: 1.173 ± 0.015	2.562AspAsn: 2.562 ± 0.022	2.558AspPro: 2.558 ± 0.022	2.126AspGln: 2.126 ± 0.02	2.756AspArg: 2.756 ± 0.027	4.408AspSer: 4.408 ± 0.03	2.327AspThr: 2.327 ± 0.021	3.223AspVal: 3.223 ± 0.026	0.81AspTrp: 0.81 ± 0.013	1.923AspTyr: 1.923 ± 0.022	0.001AspXaa: 0.001 ± 0.0
Glu	4.504GluAla: 4.504 ± 0.041	1.5GluCys: 1.5 ± 0.033	4.52GluAsp: 4.52 ± 0.03	6.888GluGlu: 6.888 ± 0.055	2.968GluPhe: 2.968 ± 0.02	3.004GluGly: 3.004 ± 0.027	1.409GluHis: 1.409 ± 0.016	5.146GluIle: 5.146 ± 0.03	6.341GluLys: 6.341 ± 0.044	6.387GluLeu: 6.387 ± 0.045	1.814GluMet: 1.814 ± 0.018	4.807GluAsn: 4.807 ± 0.035	2.583GluPro: 2.583 ± 0.026	2.832GluGln: 2.832 ± 0.026	3.902GluArg: 3.902 ± 0.029	5.046GluSer: 5.046 ± 0.033	3.869GluThr: 3.869 ± 0.029	3.621GluVal: 3.621 ± 0.028	0.811GluTrp: 0.811 ± 0.014	2.006GluTyr: 2.006 ± 0.016	0.001GluXaa: 0.001 ± 0.0
Phe	2.803PheAla: 2.803 ± 0.023	1.115PheCys: 1.115 ± 0.019	2.898PheAsp: 2.898 ± 0.023	3.068PheGlu: 3.068 ± 0.023	2.236PhePhe: 2.236 ± 0.022	2.992PheGly: 2.992 ± 0.026	1.023PheHis: 1.023 ± 0.013	2.55PheIle: 2.55 ± 0.026	2.58PheLys: 2.58 ± 0.022	3.886PheLeu: 3.886 ± 0.029	1.047PheMet: 1.047 ± 0.012	2.157PheAsn: 2.157 ± 0.019	1.748PhePro: 1.748 ± 0.018	1.73PheGln: 1.73 ± 0.017	2.06PheArg: 2.06 ± 0.023	3.663PheSer: 3.663 ± 0.028	2.283PheThr: 2.283 ± 0.021	2.59PheVal: 2.59 ± 0.021	0.616PheTrp: 0.616 ± 0.009	1.558PheTyr: 1.558 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	3.473GlyAla: 3.473 ± 0.032	1.169GlyCys: 1.169 ± 0.019	3.158GlyAsp: 3.158 ± 0.028	3.577GlyGlu: 3.577 ± 0.034	2.778GlyPhe: 2.778 ± 0.028	3.805GlyGly: 3.805 ± 0.049	1.251GlyHis: 1.251 ± 0.016	3.157GlyIle: 3.157 ± 0.026	3.657GlyLys: 3.657 ± 0.03	4.325GlyLeu: 4.325 ± 0.032	1.237GlyMet: 1.237 ± 0.016	2.725GlyAsn: 2.725 ± 0.022	2.016GlyPro: 2.016 ± 0.031	2.125GlyGln: 2.125 ± 0.02	2.975GlyArg: 2.975 ± 0.028	4.356GlySer: 4.356 ± 0.041	2.935GlyThr: 2.935 ± 0.035	2.997GlyVal: 2.997 ± 0.026	0.822GlyTrp: 0.822 ± 0.012	1.903GlyTyr: 1.903 ± 0.029	0.002GlyXaa: 0.002 ± 0.001
His	1.219HisAla: 1.219 ± 0.015	0.499HisCys: 0.499 ± 0.01	1.071HisAsp: 1.071 ± 0.014	1.384HisGlu: 1.384 ± 0.015	1.107HisPhe: 1.107 ± 0.013	1.199HisGly: 1.199 ± 0.014	0.614HisHis: 0.614 ± 0.011	1.066HisIle: 1.066 ± 0.012	1.196HisLys: 1.196 ± 0.014	1.857HisLeu: 1.857 ± 0.019	0.461HisMet: 0.461 ± 0.009	0.892HisAsn: 0.892 ± 0.012	1.062HisPro: 1.062 ± 0.015	0.967HisGln: 0.967 ± 0.014	1.179HisArg: 1.179 ± 0.014	1.537HisSer: 1.537 ± 0.016	0.881HisThr: 0.881 ± 0.013	1.173HisVal: 1.173 ± 0.013	0.265HisTrp: 0.265 ± 0.007	0.693HisTyr: 0.693 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.754IleAla: 3.754 ± 0.028	1.414IleCys: 1.414 ± 0.017	3.65IleAsp: 3.65 ± 0.025	4.367IleGlu: 4.367 ± 0.034	2.894IlePhe: 2.894 ± 0.029	3.139IleGly: 3.139 ± 0.024	1.209IleHis: 1.209 ± 0.014	3.397IleIle: 3.397 ± 0.024	3.82IleLys: 3.82 ± 0.029	4.98IleLeu: 4.98 ± 0.036	1.255IleMet: 1.255 ± 0.013	2.784IleAsn: 2.784 ± 0.021	2.717IlePro: 2.717 ± 0.022	2.328IleGln: 2.328 ± 0.023	2.962IleArg: 2.962 ± 0.022	4.846IleSer: 4.846 ± 0.03	3.008IleThr: 3.008 ± 0.029	3.308IleVal: 3.308 ± 0.026	0.705IleTrp: 0.705 ± 0.011	1.754IleTyr: 1.754 ± 0.018	0.001IleXaa: 0.001 ± 0.0
Lys	4.165LysAla: 4.165 ± 0.034	1.429LysCys: 1.429 ± 0.021	3.491LysAsp: 3.491 ± 0.025	5.201LysGlu: 5.201 ± 0.039	2.768LysPhe: 2.768 ± 0.019	2.786LysGly: 2.786 ± 0.026	1.27LysHis: 1.27 ± 0.013	4.916LysIle: 4.916 ± 0.032	6.441LysLys: 6.441 ± 0.048	6.035LysLeu: 6.035 ± 0.046	1.851LysMet: 1.851 ± 0.018	4.083LysAsn: 4.083 ± 0.031	2.715LysPro: 2.715 ± 0.026	2.568LysGln: 2.568 ± 0.022	4.075LysArg: 4.075 ± 0.031	5.248LysSer: 5.248 ± 0.035	4.155LysThr: 4.155 ± 0.031	3.478LysVal: 3.478 ± 0.023	0.826LysTrp: 0.826 ± 0.011	2.029LysTyr: 2.029 ± 0.019	0.001LysXaa: 0.001 ± 0.0
Leu	5.717LeuAla: 5.717 ± 0.039	1.535LeuCys: 1.535 ± 0.02	4.825LeuAsp: 4.825 ± 0.033	6.36LeuGlu: 6.36 ± 0.054	3.604LeuPhe: 3.604 ± 0.03	4.221LeuGly: 4.221 ± 0.029	1.749LeuHis: 1.749 ± 0.018	4.586LeuIle: 4.586 ± 0.032	6.07LeuLys: 6.07 ± 0.039	7.308LeuLeu: 7.308 ± 0.057	1.929LeuMet: 1.929 ± 0.019	4.092LeuAsn: 4.092 ± 0.028	4.08LeuPro: 4.08 ± 0.028	3.643LeuGln: 3.643 ± 0.032	4.546LeuArg: 4.546 ± 0.034	6.718LeuSer: 6.718 ± 0.044	4.673LeuThr: 4.673 ± 0.027	4.643LeuVal: 4.643 ± 0.035	0.831LeuTrp: 0.831 ± 0.011	2.201LeuTyr: 2.201 ± 0.019	0.002LeuXaa: 0.002 ± 0.001
Met	1.549MetAla: 1.549 ± 0.017	0.365MetCys: 0.365 ± 0.008	1.266MetAsp: 1.266 ± 0.015	1.637MetGlu: 1.637 ± 0.017	0.853MetPhe: 0.853 ± 0.013	1.132MetGly: 1.132 ± 0.012	0.424MetHis: 0.424 ± 0.008	1.393MetIle: 1.393 ± 0.015	1.842MetLys: 1.842 ± 0.018	1.852MetLeu: 1.852 ± 0.021	0.712MetMet: 0.712 ± 0.013	1.243MetAsn: 1.243 ± 0.013	1.059MetPro: 1.059 ± 0.014	0.842MetGln: 0.842 ± 0.012	1.263MetArg: 1.263 ± 0.014	1.909MetSer: 1.909 ± 0.018	1.399MetThr: 1.399 ± 0.014	1.176MetVal: 1.176 ± 0.013	0.218MetTrp: 0.218 ± 0.006	0.516MetTyr: 0.516 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	3.088AsnAla: 3.088 ± 0.027	1.123AsnCys: 1.123 ± 0.017	2.845AsnAsp: 2.845 ± 0.023	3.762AsnGlu: 3.762 ± 0.024	2.498AsnPhe: 2.498 ± 0.023	3.166AsnGly: 3.166 ± 0.032	1.039AsnHis: 1.039 ± 0.013	2.871AsnIle: 2.871 ± 0.023	2.953AsnLys: 2.953 ± 0.023	4.34AsnLeu: 4.34 ± 0.032	1.193AsnMet: 1.193 ± 0.013	2.498AsnAsn: 2.498 ± 0.024	2.373AsnPro: 2.373 ± 0.022	2.086AsnGln: 2.086 ± 0.018	2.519AsnArg: 2.519 ± 0.023	4.041AsnSer: 4.041 ± 0.027	2.362AsnThr: 2.362 ± 0.023	2.605AsnVal: 2.605 ± 0.022	0.71AsnTrp: 0.71 ± 0.01	1.657AsnTyr: 1.657 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	3.198ProAla: 3.198 ± 0.03	0.812ProCys: 0.812 ± 0.027	2.593ProAsp: 2.593 ± 0.022	3.594ProGlu: 3.594 ± 0.031	1.728ProPhe: 1.728 ± 0.018	2.585ProGly: 2.585 ± 0.047	0.831ProHis: 0.831 ± 0.013	2.393ProIle: 2.393 ± 0.022	3.148ProLys: 3.148 ± 0.028	3.233ProLeu: 3.233 ± 0.025	0.876ProMet: 0.876 ± 0.011	2.074ProAsn: 2.074 ± 0.022	2.748ProPro: 2.748 ± 0.038	1.747ProGln: 1.747 ± 0.02	2.215ProArg: 2.215 ± 0.022	3.701ProSer: 3.701 ± 0.032	2.591ProThr: 2.591 ± 0.028	2.868ProVal: 2.868 ± 0.025	0.475ProTrp: 0.475 ± 0.008	1.133ProTyr: 1.133 ± 0.014	0.001ProXaa: 0.001 ± 0.0
Gln	2.55GlnAla: 2.55 ± 0.022	0.747GlnCys: 0.747 ± 0.013	1.736GlnAsp: 1.736 ± 0.016	2.755GlnGlu: 2.755 ± 0.026	1.698GlnPhe: 1.698 ± 0.016	1.695GlnGly: 1.695 ± 0.02	0.842GlnHis: 0.842 ± 0.013	2.654GlnIle: 2.654 ± 0.018	3.224GlnLys: 3.224 ± 0.024	3.624GlnLeu: 3.624 ± 0.029	1.12GlnMet: 1.12 ± 0.014	2.452GlnAsn: 2.452 ± 0.02	1.721GlnPro: 1.721 ± 0.021	2.095GlnGln: 2.095 ± 0.028	2.3GlnArg: 2.3 ± 0.022	2.867GlnSer: 2.867 ± 0.025	2.121GlnThr: 2.121 ± 0.02	2.109GlnVal: 2.109 ± 0.02	0.431GlnTrp: 0.431 ± 0.008	1.076GlnTyr: 1.076 ± 0.013	0.001GlnXaa: 0.001 ± 0.0
Arg	3.327ArgAla: 3.327 ± 0.025	0.994ArgCys: 0.994 ± 0.015	2.79ArgAsp: 2.79 ± 0.025	3.651ArgGlu: 3.651 ± 0.029	2.218ArgPhe: 2.218 ± 0.02	2.651ArgGly: 2.651 ± 0.03	1.178ArgHis: 1.178 ± 0.014	3.122ArgIle: 3.122 ± 0.025	4.299ArgLys: 4.299 ± 0.032	4.517ArgLeu: 4.517 ± 0.031	1.219ArgMet: 1.219 ± 0.014	2.791ArgAsn: 2.791 ± 0.023	2.376ArgPro: 2.376 ± 0.026	2.302ArgGln: 2.302 ± 0.019	3.954ArgArg: 3.954 ± 0.039	4.173ArgSer: 4.173 ± 0.035	2.644ArgThr: 2.644 ± 0.021	2.83ArgVal: 2.83 ± 0.023	0.612ArgTrp: 0.612 ± 0.011	1.45ArgTyr: 1.45 ± 0.015	0.002ArgXaa: 0.002 ± 0.0
Ser	5.114SerAla: 5.114 ± 0.033	1.789SerCys: 1.789 ± 0.025	4.687SerAsp: 4.687 ± 0.03	5.479SerGlu: 5.479 ± 0.035	3.591SerPhe: 3.591 ± 0.025	4.681SerGly: 4.681 ± 0.035	1.479SerHis: 1.479 ± 0.017	4.409SerIle: 4.409 ± 0.031	5.006SerLys: 5.006 ± 0.034	6.305SerLeu: 6.305 ± 0.037	1.702SerMet: 1.702 ± 0.017	3.546SerAsn: 3.546 ± 0.025	3.663SerPro: 3.663 ± 0.04	3.099SerGln: 3.099 ± 0.028	4.263SerArg: 4.263 ± 0.032	8.285SerSer: 8.285 ± 0.059	4.759SerThr: 4.759 ± 0.037	4.28SerVal: 4.28 ± 0.031	0.978SerTrp: 0.978 ± 0.014	2.124SerTyr: 2.124 ± 0.02	0.001SerXaa: 0.001 ± 0.0
Thr	3.588ThrAla: 3.588 ± 0.031	1.356ThrCys: 1.356 ± 0.026	2.965ThrAsp: 2.965 ± 0.024	3.664ThrGlu: 3.664 ± 0.033	2.314ThrPhe: 2.314 ± 0.023	3.209ThrGly: 3.209 ± 0.028	1.015ThrHis: 1.015 ± 0.014	3.246ThrIle: 3.246 ± 0.028	3.47ThrLys: 3.47 ± 0.027	4.323ThrLeu: 4.323 ± 0.031	1.147ThrMet: 1.147 ± 0.014	2.364ThrAsn: 2.364 ± 0.023	2.727ThrPro: 2.727 ± 0.027	1.897ThrGln: 1.897 ± 0.016	2.676ThrArg: 2.676 ± 0.022	4.474ThrSer: 4.474 ± 0.036	4.276ThrThr: 4.276 ± 0.114	3.326ThrVal: 3.326 ± 0.033	0.621ThrTrp: 0.621 ± 0.01	1.367ThrTyr: 1.367 ± 0.018	0.002ThrXaa: 0.002 ± 0.001
Val	3.645ValAla: 3.645 ± 0.03	1.183ValCys: 1.183 ± 0.02	3.511ValAsp: 3.511 ± 0.026	4.062ValGlu: 4.062 ± 0.03	2.614ValPhe: 2.614 ± 0.022	2.879ValGly: 2.879 ± 0.03	1.157ValHis: 1.157 ± 0.014	3.153ValIle: 3.153 ± 0.023	3.734ValLys: 3.734 ± 0.03	4.565ValLeu: 4.565 ± 0.029	1.165ValMet: 1.165 ± 0.012	2.699ValAsn: 2.699 ± 0.023	2.557ValPro: 2.557 ± 0.024	2.206ValGln: 2.206 ± 0.022	2.735ValArg: 2.735 ± 0.023	4.108ValSer: 4.108 ± 0.026	3.072ValThr: 3.072 ± 0.029	3.233ValVal: 3.233 ± 0.028	0.608ValTrp: 0.608 ± 0.01	1.526ValTyr: 1.526 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.724TrpAla: 0.724 ± 0.012	0.224TrpCys: 0.224 ± 0.007	0.672TrpAsp: 0.672 ± 0.012	0.751TrpGlu: 0.751 ± 0.013	0.494TrpPhe: 0.494 ± 0.009	0.641TrpGly: 0.641 ± 0.013	0.25TrpHis: 0.25 ± 0.007	0.757TrpIle: 0.757 ± 0.012	0.88TrpLys: 0.88 ± 0.013	0.978TrpLeu: 0.978 ± 0.013	0.293TrpMet: 0.293 ± 0.007	0.742TrpAsn: 0.742 ± 0.011	0.417TrpPro: 0.417 ± 0.009	0.479TrpGln: 0.479 ± 0.009	0.689TrpArg: 0.689 ± 0.01	0.99TrpSer: 0.99 ± 0.019	0.776TrpThr: 0.776 ± 0.013	0.558TrpVal: 0.558 ± 0.01	0.178TrpTrp: 0.178 ± 0.005	0.326TrpTyr: 0.326 ± 0.006	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.693TyrAla: 1.693 ± 0.019	0.758TyrCys: 0.758 ± 0.028	1.803TyrAsp: 1.803 ± 0.017	2.057TyrGlu: 2.057 ± 0.017	1.473TyrPhe: 1.473 ± 0.018	1.776TyrGly: 1.776 ± 0.024	0.702TyrHis: 0.702 ± 0.01	1.439TyrIle: 1.439 ± 0.015	1.747TyrLys: 1.747 ± 0.018	2.49TyrLeu: 2.49 ± 0.024	0.613TyrMet: 0.613 ± 0.01	1.449TyrAsn: 1.449 ± 0.018	1.21TyrPro: 1.21 ± 0.015	1.203TyrGln: 1.203 ± 0.014	1.55TyrArg: 1.55 ± 0.015	2.278TyrSer: 2.278 ± 0.021	1.355TyrThr: 1.355 ± 0.017	1.473TyrVal: 1.473 ± 0.016	0.367TyrTrp: 0.367 ± 0.008	1.03TyrTyr: 1.03 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.002XaaPhe: 0.002 ± 0.001	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.002XaaLeu: 0.002 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.002XaaThr: 0.002 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	14.589XaaXaa: 14.589 ± 1.752

Statistics based on 17050 proteins (6837326 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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