Pacificibacter marinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pacificibacter

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3808 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y5RG73|A0A1Y5RG73_9RHOB Uncharacterized protein OS=Pacificibacter marinus OX=658057 GN=PAM7971_00376 PE=4 SV=1
MM1 pKa = 7.35GAQRR5 pKa = 11.84VLPAVVKK12 pKa = 10.23RR13 pKa = 11.84VSKK16 pKa = 10.69FSLAVMCAGALIATMGTSASDD37 pKa = 3.21AGDD40 pKa = 3.87YY41 pKa = 11.02SIDD44 pKa = 2.88WSAFDD49 pKa = 4.01WPDD52 pKa = 3.44GEE54 pKa = 4.45TGPLVRR60 pKa = 11.84TLRR63 pKa = 11.84DD64 pKa = 3.25QYY66 pKa = 11.46GFEE69 pKa = 3.9VDD71 pKa = 3.15ATVEE75 pKa = 3.96HH76 pKa = 6.59SGDD79 pKa = 3.58FISYY83 pKa = 8.95RR84 pKa = 11.84NSSGVITPTPDD95 pKa = 2.85DD96 pKa = 3.53TTIFGGNIEE105 pKa = 4.18SLILVSDD112 pKa = 4.34APPNQGAIGDD122 pKa = 3.82ARR124 pKa = 11.84IISSVSASSGGVAIRR139 pKa = 11.84VDD141 pKa = 3.52NLTIDD146 pKa = 4.22VLDD149 pKa = 4.49IDD151 pKa = 5.7ANDD154 pKa = 3.85NNSTADD160 pKa = 3.27RR161 pKa = 11.84CDD163 pKa = 4.36FITAFGDD170 pKa = 3.38NGNPTLSPVSATPTLVTGPGTGSGLTGALTANQAQCIYY208 pKa = 10.41NDD210 pKa = 4.2GPAGSPTSPNDD221 pKa = 3.18DD222 pKa = 3.61TGTVRR227 pKa = 11.84ASFPDD232 pKa = 3.35ATSSVTFWYY241 pKa = 10.15DD242 pKa = 3.06EE243 pKa = 4.41SIQNVRR249 pKa = 11.84NYY251 pKa = 11.06NVFTNYY257 pKa = 10.61DD258 pKa = 3.35PGARR262 pKa = 11.84GIGMFGNANFTVDD275 pKa = 3.7QSISLDD281 pKa = 3.32RR282 pKa = 11.84SATPTSALAGDD293 pKa = 4.03NVTYY297 pKa = 9.78TYY299 pKa = 10.94TVTNNGALPFNPNQDD314 pKa = 3.71VIIEE318 pKa = 4.24DD319 pKa = 3.89SLLGTVTCPAIAAPIAPGGTVVCSASYY346 pKa = 9.82TLTPADD352 pKa = 5.55LITGSVDD359 pKa = 3.06STATAGIGAIGQAFPVRR376 pKa = 11.84LQSNPQSLSVASAVNGPISGPQSCSPVSIFDD407 pKa = 3.63SPRR410 pKa = 11.84TQLAGSGSAGALTLSDD426 pKa = 3.84VFLFDD431 pKa = 6.19DD432 pKa = 3.64VTTDD436 pKa = 3.62VNGNPIDD443 pKa = 3.69VVFQIDD449 pKa = 3.85QISDD453 pKa = 3.15ATAVNVSTGLEE464 pKa = 3.86ARR466 pKa = 11.84MTPADD471 pKa = 3.58DD472 pKa = 4.37AFVSFHH478 pKa = 6.42LRR480 pKa = 11.84LVQDD484 pKa = 4.25GTATTANPQGTAIDD498 pKa = 3.51QSRR501 pKa = 11.84INGILVQQTDD511 pKa = 3.0VDD513 pKa = 4.1SRR515 pKa = 11.84GTSDD519 pKa = 4.44DD520 pKa = 3.54SSDD523 pKa = 3.82VVGTLDD529 pKa = 3.56PVTNIYY535 pKa = 10.3HH536 pKa = 6.76FNTAPLASFPVGNPAIAMDD555 pKa = 4.21PAKK558 pKa = 10.34TGDD561 pKa = 3.49PTNWLDD567 pKa = 4.66EE568 pKa = 4.63PNEE571 pKa = 4.4TNFDD575 pKa = 3.65NYY577 pKa = 9.01VTMEE581 pKa = 4.02FDD583 pKa = 3.46TFVEE587 pKa = 4.02ARR589 pKa = 11.84FIHH592 pKa = 7.09GYY594 pKa = 8.47TGTSTNDD601 pKa = 2.89ATRR604 pKa = 11.84GTTILLCAISNTSPDD619 pKa = 3.94VIAEE623 pKa = 4.28DD624 pKa = 4.41DD625 pKa = 5.07DD626 pKa = 4.43YY627 pKa = 11.58TASPINALAGGTAGEE642 pKa = 4.44VMANDD647 pKa = 5.04TINGLPAAFPTATLEE662 pKa = 4.28VLVEE666 pKa = 4.01ATPQNTGDD674 pKa = 3.89PVPTLEE680 pKa = 4.57VSGAAAGSVLVPAGVPAGVYY700 pKa = 8.89TIEE703 pKa = 4.14YY704 pKa = 8.38QLCDD708 pKa = 3.38AVDD711 pKa = 3.62PTDD714 pKa = 3.79CDD716 pKa = 3.45RR717 pKa = 11.84AKK719 pKa = 10.16VTVAVFDD726 pKa = 4.38GNGLDD731 pKa = 4.89FGDD734 pKa = 4.58APVSYY739 pKa = 8.88LTASHH744 pKa = 7.17GISLTPTIYY753 pKa = 10.86LGDD756 pKa = 3.96TPPDD760 pKa = 3.73SEE762 pKa = 5.39IVPQSDD768 pKa = 2.99ATATADD774 pKa = 5.27DD775 pKa = 4.61LLEE778 pKa = 4.5TDD780 pKa = 5.56DD781 pKa = 4.24EE782 pKa = 4.72DD783 pKa = 5.36AVIFPILTQGVNVTLDD799 pKa = 3.06IDD801 pKa = 4.21VTGLGYY807 pKa = 10.25LQAWIDD813 pKa = 3.85FNGDD817 pKa = 3.29GVFEE821 pKa = 4.77DD822 pKa = 4.26ALSEE826 pKa = 4.89RR827 pKa = 11.84IATDD831 pKa = 3.21LRR833 pKa = 11.84DD834 pKa = 3.67NGTGADD840 pKa = 3.59VVAGDD845 pKa = 4.4GVIQVNVTVPTDD857 pKa = 3.29ATTSTTFARR866 pKa = 11.84FRR868 pKa = 11.84YY869 pKa = 9.31GSEE872 pKa = 3.87NGLSMVSFAVDD883 pKa = 3.67GEE885 pKa = 4.5VEE887 pKa = 4.59DD888 pKa = 4.35YY889 pKa = 11.45SLLIAAADD897 pKa = 3.7LVDD900 pKa = 5.34RR901 pKa = 11.84GDD903 pKa = 3.89APASYY908 pKa = 10.48GDD910 pKa = 3.73PRR912 pKa = 11.84HH913 pKa = 6.15AVVPSIYY920 pKa = 10.32LGAGLPDD927 pKa = 3.81TEE929 pKa = 4.44VTPQNSPLADD939 pKa = 4.35ADD941 pKa = 4.49DD942 pKa = 4.31LAGDD946 pKa = 4.56DD947 pKa = 5.24DD948 pKa = 4.74EE949 pKa = 7.45DD950 pKa = 5.77SIAGFPLLVAGSTVPLTVQTNEE972 pKa = 3.98TLSIQLALGIPVSEE986 pKa = 5.38GITNLQLWIDD996 pKa = 4.32FDD998 pKa = 3.83QSGTFEE1004 pKa = 4.28PTEE1007 pKa = 4.07QIAVDD1012 pKa = 3.75YY1013 pKa = 10.62RR1014 pKa = 11.84DD1015 pKa = 3.83GGFGDD1020 pKa = 3.7TDD1022 pKa = 3.5GTFNNQISLNIAVPSDD1038 pKa = 3.03IGNGTSFARR1047 pKa = 11.84LRR1049 pKa = 11.84WSTSSAVAADD1059 pKa = 3.83PFDD1062 pKa = 3.9GFNADD1067 pKa = 5.02GEE1069 pKa = 4.66VEE1071 pKa = 4.55DD1072 pKa = 4.82YY1073 pKa = 11.21LVTLSNPNGPLVCDD1087 pKa = 3.14TGFFMVYY1094 pKa = 10.22ASNGKK1099 pKa = 9.04PILEE1103 pKa = 4.27KK1104 pKa = 10.9LQITGSPGSYY1114 pKa = 8.46TSQRR1118 pKa = 11.84TTYY1121 pKa = 10.36PPDD1124 pKa = 3.11FAGNYY1129 pKa = 9.72GITGWGWSEE1138 pKa = 3.82VDD1140 pKa = 3.17NYY1142 pKa = 10.6IYY1144 pKa = 10.94GILRR1148 pKa = 11.84NTFQLHH1154 pKa = 5.16RR1155 pKa = 11.84VTYY1158 pKa = 9.16SGAVQPVIDD1167 pKa = 4.19MAGLGLGTVGSTLEE1181 pKa = 3.96ILPNGIMIYY1190 pKa = 10.51NILGQSGRR1198 pKa = 11.84YY1199 pKa = 7.8QLLDD1203 pKa = 3.03ISDD1206 pKa = 4.24PLSPVNLGVLDD1217 pKa = 4.45AGGGAPNGLDD1227 pKa = 2.97MSYY1230 pKa = 10.89NIRR1233 pKa = 11.84DD1234 pKa = 3.53GLIYY1238 pKa = 9.83MISNGNSIYY1247 pKa = 11.34AMDD1250 pKa = 4.71PKK1252 pKa = 10.77GGTSGATTTTLVAANVPLPAGFGSSQLDD1280 pKa = 3.87SVWMDD1285 pKa = 3.23EE1286 pKa = 4.3NGFLYY1291 pKa = 10.23AYY1293 pKa = 9.73DD1294 pKa = 3.66NNSRR1298 pKa = 11.84QVFGLEE1304 pKa = 3.63VGAAGARR1311 pKa = 11.84PASYY1315 pKa = 10.7QFFAIDD1321 pKa = 3.83GTGSAPIGNDD1331 pKa = 3.1GASCRR1336 pKa = 11.84KK1337 pKa = 9.04GSLYY1341 pKa = 9.72ATSVFAEE1348 pKa = 4.62GTISGTLFEE1357 pKa = 5.07DD1358 pKa = 3.82TDD1360 pKa = 4.03NSGTLNGGEE1369 pKa = 4.33TGLSALITVALYY1381 pKa = 10.89NDD1383 pKa = 3.93NGTPTDD1389 pKa = 3.32IADD1392 pKa = 3.97DD1393 pKa = 3.92VLVQTGEE1400 pKa = 4.1TLNDD1404 pKa = 3.44GTYY1407 pKa = 11.02SFAAVDD1413 pKa = 3.27ATLTYY1418 pKa = 10.2RR1419 pKa = 11.84IEE1421 pKa = 4.14VDD1423 pKa = 3.3EE1424 pKa = 4.75TDD1426 pKa = 3.64PDD1428 pKa = 3.47IPAALSLSTTNPLTGVTVTTGADD1451 pKa = 2.99TSGQDD1456 pKa = 3.16FGFATVAAAADD1467 pKa = 3.9LSLSKK1472 pKa = 10.53LAQDD1476 pKa = 3.64TSGVPLTQADD1486 pKa = 3.6AGTAFDD1492 pKa = 4.99FVLTVTNDD1500 pKa = 3.43GPGAASDD1507 pKa = 3.59VTVIDD1512 pKa = 4.57RR1513 pKa = 11.84LPDD1516 pKa = 3.84GYY1518 pKa = 11.42SYY1520 pKa = 11.74VSDD1523 pKa = 4.12DD1524 pKa = 3.5AAATGGSYY1532 pKa = 11.04DD1533 pKa = 3.96EE1534 pKa = 5.04GTGLWTLGNIANGATASLTIRR1555 pKa = 11.84VTMNATGEE1563 pKa = 4.27HH1564 pKa = 5.73TNVAEE1569 pKa = 4.35ITGSSLPDD1577 pKa = 4.27PDD1579 pKa = 4.34SDD1581 pKa = 4.06PAVGPLTDD1589 pKa = 4.74DD1590 pKa = 4.43LSDD1593 pKa = 4.72EE1594 pKa = 4.43IADD1597 pKa = 4.34DD1598 pKa = 5.08DD1599 pKa = 4.18EE1600 pKa = 5.42AQATVTLTGAGAVISGTVFIDD1621 pKa = 3.13NGNGGGTSFDD1631 pKa = 3.49GAQNGTEE1638 pKa = 3.99IGSPAATVSVFDD1650 pKa = 4.1STNTLIDD1657 pKa = 3.89TPEE1660 pKa = 3.91VAADD1664 pKa = 4.09GAWSLVLPAGYY1675 pKa = 9.97SDD1677 pKa = 3.7VVTVVVTPDD1686 pKa = 3.17AAHH1689 pKa = 6.38LVISEE1694 pKa = 4.43TPTAFPSQTNTDD1706 pKa = 3.29TRR1708 pKa = 11.84DD1709 pKa = 3.15GRR1711 pKa = 11.84LSFTAAANTDD1721 pKa = 3.67YY1722 pKa = 11.59AGLDD1726 pKa = 3.34VGLLQSAKK1734 pKa = 10.62LNEE1737 pKa = 4.31SQDD1740 pKa = 3.37AAIAAGQVALLRR1752 pKa = 11.84HH1753 pKa = 6.47EE1754 pKa = 4.76YY1755 pKa = 10.56YY1756 pKa = 11.1ADD1758 pKa = 3.81ADD1760 pKa = 3.79GTVIFDD1766 pKa = 3.71TTVVSQAPAGAFTVALFEE1784 pKa = 6.29DD1785 pKa = 4.21NGCDD1789 pKa = 3.24GTADD1793 pKa = 3.73VVISGPLTIAPDD1805 pKa = 3.59TLICVIARR1813 pKa = 11.84VSASSAVGPNSSYY1826 pKa = 11.08IFDD1829 pKa = 4.02LTADD1833 pKa = 3.69TTYY1836 pKa = 9.34GTTGITEE1843 pKa = 3.9QDD1845 pKa = 3.33RR1846 pKa = 11.84NTDD1849 pKa = 3.34RR1850 pKa = 11.84LTADD1854 pKa = 3.5AQQGSLKK1861 pKa = 10.47LSKK1864 pKa = 9.18TVRR1867 pKa = 11.84NVTQNSVEE1875 pKa = 3.87AVANGGTLGDD1885 pKa = 3.58VLEE1888 pKa = 4.14YY1889 pKa = 10.49KK1890 pKa = 10.12IYY1892 pKa = 10.96LEE1894 pKa = 4.8NPTSLPATDD1903 pKa = 3.16VTIYY1907 pKa = 10.99DD1908 pKa = 3.59KK1909 pKa = 10.96TPPYY1913 pKa = 9.77TVLDD1917 pKa = 4.32GPIPSPITVGGSLTCTVGLPVANVAGYY1944 pKa = 10.53AGNLRR1949 pKa = 11.84WDD1951 pKa = 3.74CTGTYY1956 pKa = 10.57LPGSQGAVSFEE1967 pKa = 4.38VKK1969 pKa = 10.51ISPP1972 pKa = 4.25

Molecular weight: 203.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y5S7Q8|A0A1Y5S7Q8_9RHOB Inner membrane ABC transporter permease protein YejB OS=Pacificibacter marinus OX=658057 GN=yejB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.82IINSRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.35SLSAA44 pKa = 3.83

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3808

0

3808

1158095

29

6832

304.1

33.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.348 ± 0.055

0.907 ± 0.014

6.184 ± 0.04

5.394 ± 0.043

3.982 ± 0.028

8.201 ± 0.039

2.079 ± 0.023

5.842 ± 0.028

3.967 ± 0.036

9.814 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.024

3.064 ± 0.028

4.625 ± 0.029

3.369 ± 0.024

5.612 ± 0.04

5.864 ± 0.041

5.989 ± 0.044

7.301 ± 0.034

1.267 ± 0.016

2.281 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski