Massilia albidiflava

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411WZ87|A0A411WZ87_9BURK MarR family transcriptional regulator OS=Massilia albidiflava OX=321983 GN=EYF70_15085 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.32NLEE5 pKa = 4.6NILYY9 pKa = 9.67DD10 pKa = 3.48YY11 pKa = 10.88LAAIEE16 pKa = 4.39AAGSLIPKK24 pKa = 9.87ALGPAPDD31 pKa = 4.82AGWIDD36 pKa = 3.57AMNALEE42 pKa = 4.7QITVPDD48 pKa = 3.89DD49 pKa = 3.41LKK51 pKa = 10.58TYY53 pKa = 7.42FTKK56 pKa = 10.62INGWDD61 pKa = 3.55ADD63 pKa = 3.61SDD65 pKa = 4.1LDD67 pKa = 4.5LFEE70 pKa = 6.06PEE72 pKa = 4.78LAWGMFPLSTDD83 pKa = 3.18RR84 pKa = 11.84SIEE87 pKa = 4.12DD88 pKa = 3.47YY89 pKa = 11.6VEE91 pKa = 3.82IADD94 pKa = 4.39IVGDD98 pKa = 4.25EE99 pKa = 4.58NPDD102 pKa = 3.21YY103 pKa = 10.42WPLGFVPILADD114 pKa = 3.94GAGSWVLVNCIAASPTYY131 pKa = 9.73GAVYY135 pKa = 10.6EE136 pKa = 4.24MTHH139 pKa = 5.77CVGINRR145 pKa = 11.84IAGSLADD152 pKa = 3.97YY153 pKa = 9.83FAGLTQVVKK162 pKa = 10.96AGWKK166 pKa = 10.2VFDD169 pKa = 5.07GDD171 pKa = 4.04SSDD174 pKa = 3.28ITVDD178 pKa = 2.97GDD180 pKa = 3.65EE181 pKa = 4.51YY182 pKa = 11.78ADD184 pKa = 3.69LQAGVFGHH192 pKa = 6.13TPYY195 pKa = 9.74FTTRR199 pKa = 11.84GSDD202 pKa = 3.37QVVDD206 pKa = 3.49WRR208 pKa = 4.24

Molecular weight: 22.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411WUD8|A0A411WUD8_9BURK Chemotaxis protein OS=Massilia albidiflava OX=321983 GN=EYF70_04780 PE=4 SV=1
MM1 pKa = 7.7PRR3 pKa = 11.84GQSRR7 pKa = 11.84RR8 pKa = 11.84SRR10 pKa = 11.84PRR12 pKa = 11.84LRR14 pKa = 11.84KK15 pKa = 8.9IPRR18 pKa = 11.84TRR20 pKa = 11.84RR21 pKa = 11.84MQTSRR26 pKa = 11.84ARR28 pKa = 11.84RR29 pKa = 11.84TAPPVPVKK37 pKa = 10.26HH38 pKa = 6.33LPPMPRR44 pKa = 11.84RR45 pKa = 11.84QRR47 pKa = 11.84PRR49 pKa = 11.84HH50 pKa = 4.53GHH52 pKa = 4.88PVKK55 pKa = 10.54ASKK58 pKa = 8.6PTRR61 pKa = 11.84LPWRR65 pKa = 11.84IRR67 pKa = 11.84LPACWPCWQPTASS80 pKa = 3.63

Molecular weight: 9.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5987

0

5987

2173394

24

3625

363.0

39.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.262 ± 0.053

0.843 ± 0.009

5.555 ± 0.026

5.105 ± 0.028

3.537 ± 0.014

8.625 ± 0.044

2.235 ± 0.019

4.334 ± 0.022

3.183 ± 0.03

10.307 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.015

2.894 ± 0.027

5.227 ± 0.028

3.699 ± 0.021

6.912 ± 0.035

5.167 ± 0.029

5.425 ± 0.032

7.397 ± 0.022

1.41 ± 0.013

2.508 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski