Desulfobotulus alkaliphilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfobotulus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562RIN1|A0A562RIN1_9DELT Uncharacterized protein OS=Desulfobotulus alkaliphilus OX=622671 GN=LZ24_02601 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 10.19RR3 pKa = 11.84IVAAMVLSFFLVSSVFAATVWHH25 pKa = 6.15QNFFSTIKK33 pKa = 10.49SDD35 pKa = 3.55GYY37 pKa = 9.21EE38 pKa = 4.05QAVKK42 pKa = 10.1EE43 pKa = 4.04ALTAGVSIEE52 pKa = 4.74DD53 pKa = 3.62IVDD56 pKa = 3.3ISSKK60 pKa = 10.65LGYY63 pKa = 9.79QGLFIFASIYY73 pKa = 9.59NASGNSGDD81 pKa = 4.02VLDD84 pKa = 5.04AALEE88 pKa = 4.23TSLGQDD94 pKa = 3.73FEE96 pKa = 5.0RR97 pKa = 11.84EE98 pKa = 4.16SVLALSSADD107 pKa = 3.54LAFLVASGDD116 pKa = 3.39IYY118 pKa = 11.52VPSLSEE124 pKa = 4.66LEE126 pKa = 4.53LNYY129 pKa = 9.54LTYY132 pKa = 10.94YY133 pKa = 11.05GLALQAGDD141 pKa = 4.93EE142 pKa = 4.31EE143 pKa = 4.84VQGQGLGFGDD153 pKa = 4.49DD154 pKa = 3.51PVLAVGDD161 pKa = 3.66ITMGDD166 pKa = 3.36VGFGGMNNSSVSPAAPP182 pKa = 3.17

Molecular weight: 19.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562S646|A0A562S646_9DELT Uncharacterized protein OS=Desulfobotulus alkaliphilus OX=622671 GN=LZ24_00453 PE=3 SV=1
MM1 pKa = 7.05PTGIIEE7 pKa = 4.15QLQVLYY13 pKa = 10.67GEE15 pKa = 5.07RR16 pKa = 11.84FRR18 pKa = 11.84SQPRR22 pKa = 11.84LEE24 pKa = 4.0RR25 pKa = 11.84LILATAATEE34 pKa = 3.88QVISHH39 pKa = 6.09KK40 pKa = 10.2RR41 pKa = 11.84ISDD44 pKa = 3.14ISIDD48 pKa = 3.5HH49 pKa = 6.48SRR51 pKa = 11.84DD52 pKa = 2.83ITIALQALVKK62 pKa = 10.62QGLQMINSTTAMAVVLFIICRR83 pKa = 11.84GRR85 pKa = 11.84IYY87 pKa = 10.29QPRR90 pKa = 11.84IRR92 pKa = 11.84SSVV95 pKa = 2.93

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3369

0

3369

1138264

26

3762

337.9

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.892 ± 0.041

1.208 ± 0.018

5.398 ± 0.031

7.092 ± 0.039

4.487 ± 0.029

7.689 ± 0.044

2.262 ± 0.017

6.069 ± 0.035

5.17 ± 0.035

10.529 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.146 ± 0.024

3.129 ± 0.026

4.646 ± 0.034

3.167 ± 0.027

6.325 ± 0.039

6.004 ± 0.03

4.837 ± 0.035

6.274 ± 0.041

1.109 ± 0.014

2.568 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski