Micromonospora maris (strain DSM 45365 / JCM 31040 / NBRC 109089 / NRRL B-24793 / AB-18-032) (Verrucosispora maris)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; Micromonospora maris

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4F7N6|F4F7N6_MICM1 tRNA (Adenine-n(1)-)-methyltransferase OS=Micromonospora maris (strain DSM 45365 / JCM 31040 / NBRC 109089 / NRRL B-24793 / AB-18-032) OX=263358 GN=VAB18032_22865 PE=4 SV=1
MM1 pKa = 7.6AEE3 pKa = 3.89VATDD7 pKa = 3.89EE8 pKa = 4.37VQVWVDD14 pKa = 3.26QDD16 pKa = 3.76LCTGDD21 pKa = 3.99GLCVQYY27 pKa = 10.98APEE30 pKa = 4.07VFEE33 pKa = 4.79FDD35 pKa = 3.39VDD37 pKa = 3.3GLAYY41 pKa = 10.67VKK43 pKa = 10.93GPDD46 pKa = 4.74GEE48 pKa = 4.55LQQAPGARR56 pKa = 11.84VGVPEE61 pKa = 4.06HH62 pKa = 6.51LRR64 pKa = 11.84LEE66 pKa = 4.8VIDD69 pKa = 4.35SARR72 pKa = 11.84EE73 pKa = 3.9CPGEE77 pKa = 4.39CIHH80 pKa = 6.63VVRR83 pKa = 11.84ASDD86 pKa = 3.74GVEE89 pKa = 3.97VAGPDD94 pKa = 4.15AEE96 pKa = 4.47DD97 pKa = 3.09

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4F2I8|F4F2I8_MICM1 RelA/SpoT family protein OS=Micromonospora maris (strain DSM 45365 / JCM 31040 / NBRC 109089 / NRRL B-24793 / AB-18-032) OX=263358 GN=VAB18032_14740 PE=3 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84RR3 pKa = 11.84LARR6 pKa = 11.84LLVRR10 pKa = 11.84RR11 pKa = 11.84LPVRR15 pKa = 11.84RR16 pKa = 11.84LTRR19 pKa = 11.84VRR21 pKa = 11.84LAGLSVLRR29 pKa = 11.84LPGLAGWPVLRR40 pKa = 11.84LPVRR44 pKa = 3.73

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5998

0

5998

1968047

30

5781

328.1

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.753 ± 0.051

0.745 ± 0.01

6.099 ± 0.029

5.047 ± 0.033

2.652 ± 0.017

9.119 ± 0.03

2.201 ± 0.017

3.353 ± 0.022

1.565 ± 0.024

10.626 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.639 ± 0.013

1.858 ± 0.023

6.296 ± 0.029

2.982 ± 0.018

8.362 ± 0.036

4.726 ± 0.024

6.323 ± 0.032

8.971 ± 0.029

1.597 ± 0.014

2.085 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski