Acidiphilium rubrum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidiphilium

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3677 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6Z510|A0A1N6Z510_ACIRU tRNA-dihydrouridine synthase OS=Acidiphilium rubrum OX=526 GN=SAMN05421828_1202 PE=3 SV=1
MM1 pKa = 7.59SIFGALDD8 pKa = 3.41TSVSGMQAQSSAFTNISDD26 pKa = 5.06NIANSQTTGYY36 pKa = 10.38KK37 pKa = 10.56GVDD40 pKa = 3.34TNFINYY46 pKa = 8.68LVQSSPSSNGADD58 pKa = 3.1SVIARR63 pKa = 11.84PSYY66 pKa = 9.57TNTVQGTVTQSTNPLALAISGQGFFNVSEE95 pKa = 4.26PAASATAGGTTQSFQSQQYY114 pKa = 6.0YY115 pKa = 8.8TRR117 pKa = 11.84AGDD120 pKa = 3.5FTLNKK125 pKa = 9.68QGYY128 pKa = 8.56LVNSAGAYY136 pKa = 9.54LNAWPIDD143 pKa = 3.66TATGVVNTSSLAPIQISQATTPPVATSSVSLSASLPTTPATSPVNTQVDD192 pKa = 3.86IYY194 pKa = 11.36DD195 pKa = 3.66SLGNLQQLNLSWTQTGTNQWNLAVYY220 pKa = 10.45APGDD224 pKa = 3.9PGGSTAKK231 pKa = 10.74APIANASMTFGSDD244 pKa = 3.02GTLSALSTTTTNAAASANTAGQPATLTIPANFGNGTQNIALDD286 pKa = 3.98FGRR289 pKa = 11.84FGGTAGLTQYY299 pKa = 11.54AGTSLNLQGASQNGTPPGNFSNLSIDD325 pKa = 3.53TQGNITVNYY334 pKa = 10.37NNGFSQAVAQIPIATFDD351 pKa = 5.06APDD354 pKa = 3.73ALQNQSGQIYY364 pKa = 7.79TASQGSGSATINPVGSNGNALVTGSTEE391 pKa = 3.89SSNVDD396 pKa = 2.62IATQFTNLITAQQAYY411 pKa = 5.86TANSKK416 pKa = 10.9VITTAQQLLTTVVNMVQQ433 pKa = 2.91

Molecular weight: 44.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7BBQ8|A0A1N7BBQ8_ACIRU Peptide/nickel transport system ATP-binding protein OS=Acidiphilium rubrum OX=526 GN=SAMN05421828_13815 PE=4 SV=1
FF1 pKa = 6.72YY2 pKa = 10.34RR3 pKa = 11.84ISAKK7 pKa = 10.27RR8 pKa = 11.84GSLPEE13 pKa = 4.06NLTGIVVHH21 pKa = 6.31DD22 pKa = 3.67HH23 pKa = 6.04WKK25 pKa = 10.09PYY27 pKa = 8.06YY28 pKa = 8.73TLAGVLHH35 pKa = 6.76ALCNAHH41 pKa = 7.15HH42 pKa = 6.41LRR44 pKa = 11.84EE45 pKa = 4.15LQALIEE51 pKa = 4.4IEE53 pKa = 4.1HH54 pKa = 6.7EE55 pKa = 3.73DD56 pKa = 2.98WARR59 pKa = 11.84RR60 pKa = 11.84MQTLLRR66 pKa = 11.84RR67 pKa = 11.84ACHH70 pKa = 5.87AANLSRR76 pKa = 11.84MQGKK80 pKa = 8.44PLPPRR85 pKa = 11.84LIALIEE91 pKa = 3.96RR92 pKa = 11.84CYY94 pKa = 11.02DD95 pKa = 4.07AIVTDD100 pKa = 4.18GLLFHH105 pKa = 7.15TDD107 pKa = 3.48QSPLTQADD115 pKa = 3.38VQARR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84GRR123 pKa = 11.84VPRR126 pKa = 11.84RR127 pKa = 11.84VGHH130 pKa = 6.25NLLLRR135 pKa = 11.84LSTRR139 pKa = 11.84KK140 pKa = 9.49PDD142 pKa = 3.5VLRR145 pKa = 11.84FLSNPAVPFTNNLAEE160 pKa = 4.46RR161 pKa = 11.84DD162 pKa = 3.6GRR164 pKa = 11.84MMKK167 pKa = 10.34LRR169 pKa = 11.84QKK171 pKa = 10.45ISGGFRR177 pKa = 11.84AVTGAEE183 pKa = 3.93DD184 pKa = 3.6FAVIRR189 pKa = 11.84SFFSTAKK196 pKa = 9.12KK197 pKa = 9.16QQWDD201 pKa = 3.5VLNAINAHH209 pKa = 6.0PNVLIAQLKK218 pKa = 8.76LAA220 pKa = 4.7

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3677

0

3677

1184703

26

2478

322.2

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.203 ± 0.066

0.842 ± 0.011

5.429 ± 0.029

4.389 ± 0.042

3.588 ± 0.025

8.715 ± 0.044

2.349 ± 0.021

5.778 ± 0.026

2.529 ± 0.031

10.355 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.017

2.513 ± 0.025

5.712 ± 0.04

3.028 ± 0.025

7.128 ± 0.048

4.933 ± 0.029

5.753 ± 0.033

6.943 ± 0.031

1.278 ± 0.018

2.09 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski