Pseudomonas phage Andromeda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bifseptvirus; Pseudomonas virus Andromeda

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1SEQ2|A0A1B1SEQ2_9CAUD DNA-directed DNA polymerase OS=Pseudomonas phage Andromeda OX=1873949 GN=Andromeda_17 PE=3 SV=1
MM1 pKa = 8.33DD2 pKa = 3.45ITYY5 pKa = 10.68EE6 pKa = 4.3RR7 pKa = 11.84ITSFDD12 pKa = 3.76CDD14 pKa = 3.38STAMAEE20 pKa = 4.08ASAYY24 pKa = 10.54ACDD27 pKa = 4.05ALAHH31 pKa = 6.39AEE33 pKa = 3.95PLQVEE38 pKa = 4.87LDD40 pKa = 3.98PVPFDD45 pKa = 3.69YY46 pKa = 11.27LEE48 pKa = 4.5SEE50 pKa = 4.5GFDD53 pKa = 3.6AYY55 pKa = 9.96TGLDD59 pKa = 3.18TALSGKK65 pKa = 9.7HH66 pKa = 5.67DD67 pKa = 3.87AAMLNYY73 pKa = 9.79GFALLGDD80 pKa = 4.46DD81 pKa = 4.24EE82 pKa = 4.59

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1SEG4|A0A1B1SEG4_9CAUD Uncharacterized protein OS=Pseudomonas phage Andromeda OX=1873949 GN=Andromeda_05 PE=4 SV=1
MM1 pKa = 6.97SQEE4 pKa = 3.97KK5 pKa = 9.45PALLTRR11 pKa = 11.84GLALTGTVTGRR22 pKa = 11.84MTYY25 pKa = 9.31PQHH28 pKa = 5.48QQIPKK33 pKa = 9.81PKK35 pKa = 8.84FADD38 pKa = 4.05GALVRR43 pKa = 11.84ADD45 pKa = 3.25KK46 pKa = 10.9AKK48 pKa = 10.29CRR50 pKa = 11.84HH51 pKa = 5.97HH52 pKa = 6.42FAGGIPFGMVFAITSDD68 pKa = 2.9AFRR71 pKa = 11.84SHH73 pKa = 7.29RR74 pKa = 11.84NPEE77 pKa = 3.1PWYY80 pKa = 10.16RR81 pKa = 11.84ITGSPDD87 pKa = 2.36IPAYY91 pKa = 9.64ILPEE95 pKa = 4.28SALKK99 pKa = 9.5PAKK102 pKa = 9.74QAEE105 pKa = 4.67KK106 pKa = 10.57KK107 pKa = 9.58RR108 pKa = 11.84AAYY111 pKa = 8.4SQRR114 pKa = 11.84RR115 pKa = 11.84GG116 pKa = 3.21

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12541

31

1240

272.6

30.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.331 ± 0.601

0.925 ± 0.155

6.347 ± 0.26

5.653 ± 0.262

3.62 ± 0.163

7.79 ± 0.263

2.177 ± 0.192

4.681 ± 0.213

5.39 ± 0.323

8.109 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.248

4.099 ± 0.275

4.425 ± 0.268

4.848 ± 0.306

5.653 ± 0.333

5.382 ± 0.185

5.725 ± 0.386

6.594 ± 0.26

1.427 ± 0.109

2.775 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski