archaeon HR05

Taxonomy: cellular organisms; Archaea; unclassified Archaea

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5VBX8|A0A2H5VBX8_9ARCH Proteasome subunit beta OS=archaeon HR05 OX=2035441 GN=prcB_1 PE=3 SV=1
MM1 pKa = 7.85AGRR4 pKa = 11.84ASRR7 pKa = 11.84VRR9 pKa = 11.84GTTITVIATFLLLIIVLSTTITDD32 pKa = 3.62SLMTVYY38 pKa = 10.44SQEE41 pKa = 4.05QEE43 pKa = 3.68QTSAVEE49 pKa = 4.34CNGHH53 pKa = 6.5IIEE56 pKa = 5.84RR57 pKa = 11.84GDD59 pKa = 3.47FDD61 pKa = 4.28NDD63 pKa = 3.0EE64 pKa = 4.05VDD66 pKa = 3.43EE67 pKa = 4.6VKK69 pKa = 10.78VDD71 pKa = 3.37GTVYY75 pKa = 10.85EE76 pKa = 5.14DD77 pKa = 3.83GDD79 pKa = 5.09SIILDD84 pKa = 3.46GNTYY88 pKa = 5.77TTRR91 pKa = 11.84IVTVYY96 pKa = 10.44LFNGTNDD103 pKa = 2.94NDD105 pKa = 4.44FIVGRR110 pKa = 11.84NVSDD114 pKa = 4.23IIRR117 pKa = 11.84GKK119 pKa = 10.58EE120 pKa = 3.65GDD122 pKa = 3.75DD123 pKa = 4.04FIVGFGDD130 pKa = 3.38NDD132 pKa = 3.8SLYY135 pKa = 11.13GDD137 pKa = 4.57DD138 pKa = 6.25LFGEE142 pKa = 4.93DD143 pKa = 4.76GNDD146 pKa = 3.7TITGGNDD153 pKa = 3.11ILCGNAGYY161 pKa = 10.61DD162 pKa = 3.48HH163 pKa = 7.61LYY165 pKa = 10.98GDD167 pKa = 4.86MIGGGDD173 pKa = 3.94GNDD176 pKa = 4.03TISGGDD182 pKa = 3.67DD183 pKa = 2.84ILYY186 pKa = 10.64GGEE189 pKa = 4.53DD190 pKa = 3.57NDD192 pKa = 4.17EE193 pKa = 4.2LVGDD197 pKa = 5.1DD198 pKa = 4.26LSGWDD203 pKa = 3.97GDD205 pKa = 5.35DD206 pKa = 5.15NITGNNDD213 pKa = 2.91VLEE216 pKa = 4.94GGTGDD221 pKa = 3.32DD222 pKa = 3.31WLYY225 pKa = 11.76GDD227 pKa = 4.67MLHH230 pKa = 7.15GGVGSDD236 pKa = 4.09NITGGKK242 pKa = 7.28DD243 pKa = 3.01TLYY246 pKa = 10.93GGEE249 pKa = 4.24GNDD252 pKa = 3.57RR253 pKa = 11.84LFGDD257 pKa = 4.43SIGGGVGNNTITGGNDD273 pKa = 3.07TLDD276 pKa = 3.9GGDD279 pKa = 4.81GNDD282 pKa = 3.42EE283 pKa = 4.39LYY285 pKa = 11.14GDD287 pKa = 4.21GMEE290 pKa = 5.23GGDD293 pKa = 4.51GNDD296 pKa = 4.0TIKK299 pKa = 11.11GGADD303 pKa = 3.18TLDD306 pKa = 3.96GGDD309 pKa = 3.87GNDD312 pKa = 3.25WLIGDD317 pKa = 5.62RR318 pKa = 11.84IWGWDD323 pKa = 3.35GDD325 pKa = 4.16DD326 pKa = 4.5TISGGDD332 pKa = 3.33DD333 pKa = 3.15TLYY336 pKa = 11.22GGIGNDD342 pKa = 3.36HH343 pKa = 7.3LYY345 pKa = 11.32GDD347 pKa = 4.64MIGGEE352 pKa = 4.29DD353 pKa = 4.45GYY355 pKa = 10.34NTITGGKK362 pKa = 7.04DD363 pKa = 2.87TLYY366 pKa = 10.93GGEE369 pKa = 4.24GNDD372 pKa = 3.57RR373 pKa = 11.84LFGDD377 pKa = 4.43SIGGGVGNNTITGGNDD393 pKa = 3.07TLDD396 pKa = 3.9GGDD399 pKa = 4.81GNDD402 pKa = 3.42EE403 pKa = 4.39LYY405 pKa = 11.14GDD407 pKa = 4.21GMEE410 pKa = 5.23GGDD413 pKa = 4.51GNDD416 pKa = 4.0TIKK419 pKa = 11.11GGADD423 pKa = 3.18TLDD426 pKa = 3.96GGDD429 pKa = 3.87GNDD432 pKa = 3.25WLIGDD437 pKa = 5.62RR438 pKa = 11.84IWGWDD443 pKa = 3.35GDD445 pKa = 4.16DD446 pKa = 4.5TISGGDD452 pKa = 3.33DD453 pKa = 3.15TLYY456 pKa = 11.22GGIGNDD462 pKa = 3.36HH463 pKa = 7.3LYY465 pKa = 11.32GDD467 pKa = 4.64MIGGEE472 pKa = 4.29DD473 pKa = 4.45GYY475 pKa = 10.34NTITGGKK482 pKa = 7.04DD483 pKa = 2.87TLYY486 pKa = 10.93GGEE489 pKa = 4.24GNDD492 pKa = 3.57RR493 pKa = 11.84LFGDD497 pKa = 4.43SIGGGVGNNTITGGNDD513 pKa = 3.07TLDD516 pKa = 3.9GGDD519 pKa = 4.81GNDD522 pKa = 3.4EE523 pKa = 4.51LYY525 pKa = 11.25GDD527 pKa = 4.95GLFGWGGDD535 pKa = 3.38DD536 pKa = 3.25TAYY539 pKa = 10.78GGNDD543 pKa = 3.64TINAKK548 pKa = 10.67DD549 pKa = 3.57NADD552 pKa = 3.65NNDD555 pKa = 4.39NIDD558 pKa = 2.88GDD560 pKa = 4.48YY561 pKa = 10.86IVNAEE566 pKa = 4.32IATLDD571 pKa = 3.67NQDD574 pKa = 3.35ICASDD579 pKa = 4.18PDD581 pKa = 4.19PEE583 pKa = 4.66VNCEE587 pKa = 3.63YY588 pKa = 11.38DD589 pKa = 4.83DD590 pKa = 4.9IYY592 pKa = 11.61TLATLSTGTDD602 pKa = 3.5PVQLGEE608 pKa = 4.08PVQITFSSTVDD619 pKa = 3.37NPVKK623 pKa = 9.79ITSLTVTTPTDD634 pKa = 3.73SICTYY639 pKa = 10.12NSLPIIVPEE648 pKa = 4.35NGTFTATYY656 pKa = 8.44PADD659 pKa = 4.97FIGIDD664 pKa = 4.33CNTDD668 pKa = 2.83TAGTYY673 pKa = 7.39TVEE676 pKa = 5.16AITEE680 pKa = 4.12VGDD683 pKa = 4.6PITTNFTISFQVVPEE698 pKa = 5.0AIAGALGIVGTGFISMLLYY717 pKa = 10.58RR718 pKa = 11.84RR719 pKa = 11.84VSKK722 pKa = 11.07GKK724 pKa = 10.79GKK726 pKa = 10.34DD727 pKa = 3.08KK728 pKa = 11.38GKK730 pKa = 10.82DD731 pKa = 3.27NVASSS736 pKa = 3.46

Molecular weight: 76.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5VCK6|A0A2H5VCK6_9ARCH Hypoxic response protein 1 OS=archaeon HR05 OX=2035441 GN=hrp1 PE=4 SV=1
MM1 pKa = 7.81GSRR4 pKa = 11.84KK5 pKa = 7.97TKK7 pKa = 8.99PRR9 pKa = 11.84KK10 pKa = 8.81NRR12 pKa = 11.84LMKK15 pKa = 9.39KK16 pKa = 7.91TKK18 pKa = 10.09QNSPVPLWVILKK30 pKa = 7.0TNRR33 pKa = 11.84RR34 pKa = 11.84VRR36 pKa = 11.84RR37 pKa = 11.84NPQQRR42 pKa = 11.84HH43 pKa = 3.83WRR45 pKa = 11.84RR46 pKa = 11.84SSVDD50 pKa = 3.09VGG52 pKa = 3.49

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

721

0

721

177987

29

1023

246.9

27.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.739 ± 0.097

1.087 ± 0.039

6.01 ± 0.089

6.504 ± 0.092

3.1 ± 0.065

7.024 ± 0.1

1.965 ± 0.042

8.352 ± 0.088

5.748 ± 0.082

9.257 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.488 ± 0.049

3.999 ± 0.068

3.381 ± 0.057

1.868 ± 0.042

6.553 ± 0.084

7.09 ± 0.091

4.21 ± 0.062

7.907 ± 0.078

0.827 ± 0.032

3.89 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski