beta proteobacterium MWH-P2sevCIIIb

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0XHM2|A0A2T0XHM2_9PROT Phosphonate degradation associated HDIG domain protein OS=beta proteobacterium MWH-P2sevCIIIb OX=323426 GN=BCM14_1249 PE=4 SV=1
MM1 pKa = 7.32SAITGLSSLLDD12 pKa = 3.37QSVSSVQAQIATTQNQLATGIKK34 pKa = 7.76QTNPAIQGIVTRR46 pKa = 11.84LSANISQYY54 pKa = 11.53SSIGTNITAAQNVVAVGQTSLTTISSLISQMQQLATQAASGGLSTTDD101 pKa = 3.36LASLNTTFTALASQISAAVSNASINSTTVLSTGGGLTVQIGLTTAASSTAAFGTLGLDD159 pKa = 3.31TSVVSVATALSISTGTAASASITSLTALLTTVSTGQSNLNAYY201 pKa = 9.35SVSLAADD208 pKa = 3.58QSAVTSLGTNLQSTINSLQAVDD230 pKa = 3.85QTALQAQLQQLNNQQSVDD248 pKa = 3.95YY249 pKa = 10.51YY250 pKa = 11.2LVSQMNTAAQAVLSIFRR267 pKa = 4.24

Molecular weight: 26.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0XHT5|A0A2T0XHT5_9PROT G3E family GTPase OS=beta proteobacterium MWH-P2sevCIIIb OX=323426 GN=BCM14_1295 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2981

0

2981

983088

30

6419

329.8

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.668 ± 0.047

0.978 ± 0.014

5.266 ± 0.039

5.066 ± 0.049

3.848 ± 0.028

7.797 ± 0.07

2.169 ± 0.027

5.809 ± 0.03

4.368 ± 0.039

10.541 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.027

3.329 ± 0.036

4.669 ± 0.033

4.129 ± 0.027

5.463 ± 0.048

6.258 ± 0.075

5.611 ± 0.063

7.493 ± 0.033

1.371 ± 0.018

2.52 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski