Enterobacteria phage RB51 (Bacteriophage RB51)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus; unclassified Tequatrovirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C3V2M6|C3V2M6_BPR51 Gp32 OS=Enterobacteria phage RB51 OX=10693 GN=32 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.03VDD5 pKa = 3.23IDD7 pKa = 3.63IVDD10 pKa = 4.09FEE12 pKa = 4.8YY13 pKa = 10.57IEE15 pKa = 4.24EE16 pKa = 4.61IIRR19 pKa = 11.84NRR21 pKa = 11.84YY22 pKa = 8.16PEE24 pKa = 4.11LSITSIHH31 pKa = 7.78DD32 pKa = 3.56DD33 pKa = 3.46PNYY36 pKa = 10.52CNFSIVIEE44 pKa = 4.78GPLEE48 pKa = 4.19DD49 pKa = 4.3LTRR52 pKa = 11.84FMANEE57 pKa = 4.09YY58 pKa = 10.0CDD60 pKa = 4.77GMDD63 pKa = 3.94SEE65 pKa = 5.3DD66 pKa = 3.5AEE68 pKa = 4.76FYY70 pKa = 10.28MGLIEE75 pKa = 4.4QQ76 pKa = 4.14

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3V2Q0|C3V2Q0_BPR51 Nuclear disruption protein OS=Enterobacteria phage RB51 OX=10693 GN=ndd PE=4 SV=1
MM1 pKa = 7.56SNFHH5 pKa = 6.65NEE7 pKa = 3.52HH8 pKa = 5.36VMQFYY13 pKa = 11.04RR14 pKa = 11.84NNFKK18 pKa = 10.36TKK20 pKa = 10.22GVFGRR25 pKa = 4.12

Molecular weight: 3.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

0

273

52876

25

1289

193.7

22.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.292 ± 0.185

1.093 ± 0.076

6.271 ± 0.111

7.258 ± 0.15

4.473 ± 0.097

6.029 ± 0.203

1.715 ± 0.078

7.639 ± 0.146

7.907 ± 0.219

7.389 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.097

5.713 ± 0.14

3.389 ± 0.104

3.389 ± 0.088

4.166 ± 0.085

6.65 ± 0.167

5.713 ± 0.211

6.44 ± 0.114

1.42 ± 0.058

4.325 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski