Orf virus (ORFV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Parapoxvirus

Average proteome isoelectric point is 7.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1AXF0|F1AXF0_ORFV PP22 OS=Orf virus OX=10258 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84VGVRR6 pKa = 11.84HH7 pKa = 6.09SPLYY11 pKa = 10.02TPSNMCMMDD20 pKa = 3.55IEE22 pKa = 5.4DD23 pKa = 3.94STDD26 pKa = 3.62TEE28 pKa = 4.46DD29 pKa = 3.32STMEE33 pKa = 3.86KK34 pKa = 9.89EE35 pKa = 4.46YY36 pKa = 10.81TSTATGDD43 pKa = 3.53ADD45 pKa = 3.65GLNVSVALIGEE56 pKa = 4.68GVSIPLSYY64 pKa = 10.29IGLGFNPSLTDD75 pKa = 3.71GYY77 pKa = 11.07LYY79 pKa = 11.37VNVSSRR85 pKa = 11.84APWDD89 pKa = 3.45QQTLDD94 pKa = 3.94LSANDD99 pKa = 3.32GWGIKK104 pKa = 9.29QVLEE108 pKa = 4.07KK109 pKa = 10.42EE110 pKa = 3.89ILAIQIGCDD119 pKa = 3.07NQKK122 pKa = 10.15FPEE125 pKa = 4.71EE126 pKa = 4.17PTTTQPPSPVTTTLSSTTLDD146 pKa = 3.98PNDD149 pKa = 4.4KK150 pKa = 9.4NTDD153 pKa = 3.51TTPTTTGASVDD164 pKa = 3.8GKK166 pKa = 10.85RR167 pKa = 11.84NPDD170 pKa = 3.66DD171 pKa = 4.45FDD173 pKa = 4.67FSLIVDD179 pKa = 4.24PRR181 pKa = 11.84CVTSVNLHH189 pKa = 5.75FEE191 pKa = 4.09IKK193 pKa = 9.72DD194 pKa = 3.02ACMDD198 pKa = 3.79HH199 pKa = 6.93KK200 pKa = 10.83EE201 pKa = 4.09SSPLSLL207 pKa = 4.73

Molecular weight: 22.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1AXC8|F1AXC8_ORFV PP37 OS=Orf virus OX=10258 PE=4 SV=1
MM1 pKa = 7.13LHH3 pKa = 6.37ARR5 pKa = 11.84APRR8 pKa = 11.84GARR11 pKa = 11.84ARR13 pKa = 11.84GRR15 pKa = 11.84TAPLPGADD23 pKa = 3.44LRR25 pKa = 11.84ARR27 pKa = 11.84RR28 pKa = 11.84PGALPRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84APRR39 pKa = 11.84GRR41 pKa = 11.84ARR43 pKa = 11.84ARR45 pKa = 11.84GRR47 pKa = 11.84LRR49 pKa = 11.84GRR51 pKa = 11.84AQVPGVSGALRR62 pKa = 11.84RR63 pKa = 11.84GGRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84GGVLARR74 pKa = 11.84GGAAAAGAPLRR85 pKa = 11.84ARR87 pKa = 11.84LRR89 pKa = 11.84RR90 pKa = 11.84QRR92 pKa = 11.84GRR94 pKa = 11.84LRR96 pKa = 11.84LPRR99 pKa = 11.84AGHH102 pKa = 5.46GPARR106 pKa = 11.84RR107 pKa = 11.84VRR109 pKa = 11.84ATGARR114 pKa = 11.84ALRR117 pKa = 11.84EE118 pKa = 4.1PGGAAPSGGARR129 pKa = 11.84PRR131 pKa = 11.84ADD133 pKa = 2.8AAAARR138 pKa = 11.84GGRR141 pKa = 11.84RR142 pKa = 11.84APRR145 pKa = 11.84VRR147 pKa = 11.84VRR149 pKa = 11.84GIPAPEE155 pKa = 3.72RR156 pKa = 11.84GGAHH160 pKa = 7.16KK161 pKa = 10.66

Molecular weight: 16.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

259

0

259

70216

53

1161

271.1

29.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.275 ± 0.264

2.37 ± 0.085

4.721 ± 0.151

5.324 ± 0.158

3.703 ± 0.174

6.113 ± 0.178

2.833 ± 0.199

3.056 ± 0.166

3.206 ± 0.157

8.803 ± 0.164

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.114

2.719 ± 0.145

5.234 ± 0.156

2.44 ± 0.107

10.23 ± 0.32

8.521 ± 0.305

5.442 ± 0.162

8.198 ± 0.208

0.782 ± 0.054

2.324 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski