Paludisphaera borealis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Isosphaerales; Isosphaeraceae; Paludisphaera

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7CPW8|A0A1U7CPW8_9BACT Acetolactate synthase OS=Paludisphaera borealis OX=1387353 GN=ilvB PE=3 SV=1
MM1 pKa = 7.46PRR3 pKa = 11.84WSDD6 pKa = 3.48DD7 pKa = 4.91DD8 pKa = 4.06EE9 pKa = 5.96GGWDD13 pKa = 3.41PADD16 pKa = 3.64EE17 pKa = 4.34VEE19 pKa = 4.62EE20 pKa = 4.53APGDD24 pKa = 4.07DD25 pKa = 3.89EE26 pKa = 6.66DD27 pKa = 4.16VTTPCPYY34 pKa = 9.85CRR36 pKa = 11.84RR37 pKa = 11.84PIYY40 pKa = 10.08DD41 pKa = 3.52DD42 pKa = 4.35SEE44 pKa = 4.29RR45 pKa = 11.84CPACAAFLSRR55 pKa = 11.84EE56 pKa = 4.1DD57 pKa = 3.54EE58 pKa = 4.64PYY60 pKa = 10.67RR61 pKa = 11.84KK62 pKa = 9.11PWWLLVGVAVCLYY75 pKa = 10.51AVYY78 pKa = 10.03RR79 pKa = 11.84WVVRR83 pKa = 11.84FF84 pKa = 3.66

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7CPU7|A0A1U7CPU7_9BACT Carboxymethylenebutenolidase OS=Paludisphaera borealis OX=1387353 GN=clcD PE=4 SV=1
MM1 pKa = 7.13TSAVLVVALSVSGQLFHH18 pKa = 7.61KK19 pKa = 7.67STPVATGKK27 pKa = 9.95VLPAAQAPSKK37 pKa = 8.86ITPYY41 pKa = 10.38VVAPAPQAPAKK52 pKa = 9.96IAPAPQAPAKK62 pKa = 9.96IAPAPQAPAKK72 pKa = 9.96IAPAPQAPAKK82 pKa = 9.83IAPAPRR88 pKa = 11.84LRR90 pKa = 11.84PRR92 pKa = 11.84SPRR95 pKa = 11.84PLRR98 pKa = 11.84LRR100 pKa = 11.84PRR102 pKa = 11.84SPRR105 pKa = 11.84PLRR108 pKa = 11.84LRR110 pKa = 11.84PRR112 pKa = 11.84SPRR115 pKa = 11.84PPRR118 pKa = 11.84LRR120 pKa = 11.84PRR122 pKa = 11.84SPRR125 pKa = 11.84PPRR128 pKa = 11.84LRR130 pKa = 11.84PRR132 pKa = 11.84SPRR135 pKa = 11.84PPRR138 pKa = 11.84LRR140 pKa = 11.84PRR142 pKa = 11.84SPRR145 pKa = 11.84PPRR148 pKa = 11.84LRR150 pKa = 11.84PRR152 pKa = 11.84SPRR155 pKa = 11.84PLRR158 pKa = 11.84LRR160 pKa = 11.84PRR162 pKa = 11.84SPRR165 pKa = 11.84PLRR168 pKa = 11.84LRR170 pKa = 11.84PRR172 pKa = 11.84SPRR175 pKa = 11.84PLRR178 pKa = 11.84LRR180 pKa = 11.84PRR182 pKa = 11.84SPRR185 pKa = 11.84PPRR188 pKa = 11.84LRR190 pKa = 11.84PRR192 pKa = 11.84SPRR195 pKa = 11.84PLRR198 pKa = 11.84LRR200 pKa = 11.84PRR202 pKa = 11.84SPRR205 pKa = 11.84PLRR208 pKa = 11.84LRR210 pKa = 11.84PRR212 pKa = 11.84SPRR215 pKa = 11.84PLRR218 pKa = 11.84LRR220 pKa = 11.84PRR222 pKa = 11.84SPRR225 pKa = 11.84PPRR228 pKa = 11.84LRR230 pKa = 11.84PRR232 pKa = 11.84SPRR235 pKa = 11.84PLRR238 pKa = 11.84LRR240 pKa = 11.84PRR242 pKa = 11.84SPRR245 pKa = 11.84PPSTPRR251 pKa = 11.84LLRR254 pKa = 11.84LRR256 pKa = 11.84SKK258 pKa = 11.09LLLNIDD264 pKa = 3.62

Molecular weight: 30.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5693

0

5693

2148798

30

3784

377.4

41.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.516 ± 0.043

0.899 ± 0.01

6.001 ± 0.028

5.67 ± 0.034

3.53 ± 0.018

8.523 ± 0.055

2.039 ± 0.016

4.361 ± 0.022

3.436 ± 0.028

10.053 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.014

2.623 ± 0.03

6.096 ± 0.028

3.07 ± 0.02

7.641 ± 0.046

5.871 ± 0.028

5.274 ± 0.036

7.641 ± 0.03

1.541 ± 0.016

2.311 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski