Megasphaera paucivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9Y117|A0A1G9Y117_9FIRM Lon protease OS=Megasphaera paucivorans OX=349095 GN=lon PE=2 SV=1
MM1 pKa = 8.03AEE3 pKa = 3.78EE4 pKa = 4.12MMEE7 pKa = 4.29EE8 pKa = 4.19VPVIVIEE15 pKa = 4.3DD16 pKa = 3.65EE17 pKa = 4.26EE18 pKa = 4.93GNEE21 pKa = 4.12VYY23 pKa = 10.77YY24 pKa = 10.91HH25 pKa = 6.99EE26 pKa = 4.73EE27 pKa = 3.57TSLEE31 pKa = 3.9YY32 pKa = 10.43DD33 pKa = 3.39GKK35 pKa = 10.86KK36 pKa = 10.31FSILVRR42 pKa = 11.84YY43 pKa = 7.95TEE45 pKa = 4.52DD46 pKa = 3.41EE47 pKa = 4.23NGEE50 pKa = 3.95EE51 pKa = 4.97AIPEE55 pKa = 4.12DD56 pKa = 4.34AEE58 pKa = 4.35TIIARR63 pKa = 11.84VEE65 pKa = 4.13MEE67 pKa = 4.95DD68 pKa = 4.39GEE70 pKa = 4.49PLYY73 pKa = 10.56VAPTDD78 pKa = 3.72EE79 pKa = 4.33EE80 pKa = 4.59FEE82 pKa = 4.55AVLQEE87 pKa = 4.03YY88 pKa = 10.42EE89 pKa = 4.27KK90 pKa = 10.7MFNEE94 pKa = 4.55EE95 pKa = 3.61

Molecular weight: 11.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9WPE4|A0A1G9WPE4_9FIRM Ion-translocating oxidoreductase complex subunit A OS=Megasphaera paucivorans OX=349095 GN=rnfA PE=3 SV=1
MM1 pKa = 7.4VIQMILFVSITTGVAVFLLIFMLFVRR27 pKa = 11.84RR28 pKa = 11.84SSTRR32 pKa = 11.84YY33 pKa = 7.02QVRR36 pKa = 11.84NRR38 pKa = 11.84VQGLQAVDD46 pKa = 3.08IMQTGKK52 pKa = 9.64MYY54 pKa = 10.55QEE56 pKa = 4.11QRR58 pKa = 11.84PHH60 pKa = 6.22RR61 pKa = 11.84SLRR64 pKa = 11.84ISLLRR69 pKa = 11.84IWDD72 pKa = 3.96WIMFIVGNRR81 pKa = 11.84VKK83 pKa = 11.16AMTPQALYY91 pKa = 10.24RR92 pKa = 11.84ALDD95 pKa = 3.69QRR97 pKa = 11.84ITWAGKK103 pKa = 8.68QYY105 pKa = 10.28IWNAGTFFFVMVLCGTGMVVLSLGFIFTDD134 pKa = 3.5VLPLMQRR141 pKa = 11.84LMIVLVMFFCGMFLPWFCLSVMITKK166 pKa = 10.21RR167 pKa = 11.84RR168 pKa = 11.84QGINAQLSDD177 pKa = 3.97MMDD180 pKa = 3.96LVCVSVQAGLSFDD193 pKa = 3.65AALTKK198 pKa = 10.6VGEE201 pKa = 4.18RR202 pKa = 11.84MKK204 pKa = 11.24GPMADD209 pKa = 3.23EE210 pKa = 4.39TNRR213 pKa = 11.84MLRR216 pKa = 11.84DD217 pKa = 3.08VRR219 pKa = 11.84MGMTRR224 pKa = 11.84RR225 pKa = 11.84QSLTAMANRR234 pKa = 11.84CHH236 pKa = 6.84VDD238 pKa = 3.05SVYY241 pKa = 11.11LFVAAVIQTEE251 pKa = 4.27SLGVSLANMLKK262 pKa = 9.72IQADD266 pKa = 3.59NMRR269 pKa = 11.84DD270 pKa = 3.02RR271 pKa = 11.84HH272 pKa = 5.08RR273 pKa = 11.84QMIRR277 pKa = 11.84EE278 pKa = 4.11KK279 pKa = 11.18AMKK282 pKa = 10.36LPVKK286 pKa = 10.33LVFPLAFFIFPTIFIVVLGPLVLTVLTQLKK316 pKa = 10.94GNFF319 pKa = 3.48

Molecular weight: 36.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2723

0

2723

847563

11

4052

311.3

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.164 ± 0.052

1.463 ± 0.023

5.269 ± 0.038

6.101 ± 0.047

4.073 ± 0.038

7.222 ± 0.05

2.265 ± 0.024

7.981 ± 0.042

6.258 ± 0.047

8.948 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.173 ± 0.025

4.241 ± 0.041

3.715 ± 0.026

3.71 ± 0.033

4.354 ± 0.034

5.653 ± 0.036

5.653 ± 0.041

6.977 ± 0.041

0.999 ± 0.018

3.78 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski